Thermal stability for the effective use of commercial catalase

Miłek, Justyna; Wójcik, Marek J.; Verschelde, W

doi:10.2478/pjct-2014-0073

Cited by 16 publications

(8 citation statements)

References 33 publications

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“…Anti-wrinkle formation, and as a modifier of skin smoothness [68] Vitamins As a food preservative and bread improver, protective activity against heart diseases, reduced the risk of colorectal adenomas and prostate cancer, reduction of thyroid hormone levels [69][70][71][72][73] Flavonoids As cancer preventive agents, protection against type 2 diabetes Functional food additive [74][75][76][77] Carotenoid Anticancer agents, additive to cosmetics and multivitamin preparation Food colouring agent, pro-vitamin A in food and animal feed [29,38,78] PUFA Prevention of heart and inflammatory diseases [79,80] Synthetic Antioxidants…”

Section: Glutathionementioning

confidence: 99%

Antioxidant Production in Dunaliella

2021

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Microalgae have become an attractive natural source of a diverse range of biomolecules, including enzymatic and non-enzymatic antioxidants; nevertheless, economically sustainable production of such compounds from microalgae biomass is still challenging. The main hurdles are: (a) increasing microalgae yield; (b) achieving optimal cultivation conditions; (c) energy-efficient and cost-effective downstream processing (extraction and purification); (d) optimal storage of post-processed antioxidant molecules. This review provides a detailed overview of enzymatic and non-enzymatic antioxidants in the cellular metabolism of the commercially important microalgae Dunaliella, industrial applications of antioxidant enzymes, strategies to enhanced antioxidant accumulation in cells, and the opportunities and limitations of current technologies for antioxidant enzymes production from microalgae biomass as an alternative to common microbial sources.

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Section: Glutathionementioning

confidence: 99%

Antioxidant Production in Dunaliella

2021

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“…Shrimp shell chitosan powder with an average molecular weight of 10 7 g mol −1 was obtained from Primex (Iceland), and further deacetylated using hot sodium hydroxide [ 67 ] to a degree of deacetylation (DD) of 89%, measured by titration. Terminox Ultra 50L catalase (TU‐CAT), a liquid product containing fungal Scytalidium thermophilum catalase [ 47,68 ] was received from Novozymes A/S (Bagsvaerd, Denmark) and used as supplied. Glacial acetic acid, sodium acetate, and 35% hydrogen peroxide solution were purchased from Fisher Scientific (Hampton, NH, USA).…”

Section: Methodsmentioning

confidence: 99%

“…The present work introduces a straightforward approach for enzyme immobilization by coating chitosan entrapped catalase onto cellulosic yarn resulting in a sustainable biocatalytic textile that is capable of simultaneous liquid transport and catalytic reaction. As a readily available, ultra‐fast enzyme (turnover frequency of 1 to 4 × 10 7 s −1 )[ 46 ] that catalyzes the decomposition of hydrogen peroxide to form water and oxygen (2H 2 O 2 ↔ H 2 O + O 2 ) at moderate temperatures, [ 47 ] catalase (E.C. 1.11.1.6) is a useful model for exploring the performance of controlled liquid flow biocatalytic yarn systems in which mass transfer occurs among three reaction phases: immobilized enzyme (solid), dissolved hydrogen peroxide substrate (liquid), and evolved oxygen product (gas).…”

Section: Introductionmentioning

confidence: 99%

Biocatalytic Yarn for Peroxide Decomposition with Controlled Liquid Transport

Yuan

Zhang

Bilheux

et al. 2021

Adv Materials Inter

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A robust biocatalytic yarn with controllable liquid transport properties is created by coating thin layers of chitosan containing catalase onto a cellulosic yarn. The resulting material integrates enzyme catalytic functionality with protective coating properties of chitosan and structural functionality of the textile. Mild immobilization conditions and good affinity between the two polysaccharides minimize enzyme inactivation during the preparation steps and prevent enzyme from leaching during peroxide decomposition testing and washing, providing a novel and versatile enzyme immobilization strategy. The catalytic efficiency of enzymes in a reaction containing solid, liquid, and gas phases is facilitated when dissolved enzyme substrate is transported by liquid flowing through the coated textile structure. The flow‐through configuration decomposes at least two times more peroxide in a twenty‐times smaller reaction zone volume compared to a stirred tank configuration. Liquid transport through the yarn and liquid spatial distribution within the yarn are investigated by in situ neutron radiography and neutron computed tomography, revealing a constrained wicking mechanism that benefits biocatalytic yarn performance. This new class of sustainable and flexible biocatalytic textile matrices has beneficial multifunctional properties, not previously described, that are applicable for numerous small‐ and large‐scale applications including controlled flow reactors and reactive filtration.

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“…Therefore, the estimated E r and E d parameters can be used in the design and modeling of the biotransformation process when using an enzyme membrane with horseradish peroxidase. In the literature, fixed bed flow bioreactors were analyzed [32], in which the value of deactivation energy E d obtained in a classical reactor study [33] was used.…”

Section: The Values Of the Activation Energy E R And The Deactivation...mentioning

confidence: 99%

The Energies of Activation and Deactivation of 2,4-Dichlorophenol Degradation by Horseradish Peroxidase Immobilized on the Modified Nanofibrous Membrane

Miłek

2024

Applied Sciences

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Chlorophenol compounds pose a health risk to many organisms due to their toxicity. The present paper presents the estimation of the activation and deactivation energies and the optimum temperatures of 2,4-dichlorophenol degradation by horseradish peroxidase (HRP). The activities of horseradish peroxidase depending on temperature were analyzed. In a mathematical model, describing 2,4-dichlorophenol degradation by HRP was assumed that both the 2,4-dichlorophenol degradation and the deactivation of HRP were first-order reactions by the enzyme concentration. The parameters of the optimum temperatures Topt, the activation energies Er, and the deactivation energies Ed in the process of 2,4-dichlorophenol degradation by HRP immobilized on a modified nanofibrous membrane were determined kd t1/2were determined for HRP ∆H# = ∆S#, the change in ∆G#∆Hd#∆Sd#, and the change in ∆Gd# for deactivated HRP were determined at 25 °C.

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Thermal stability for the effective use of commercial catalase

Cited by 16 publications

References 33 publications

Antioxidant Production in Dunaliella

Antioxidant Production in Dunaliella

Biocatalytic Yarn for Peroxide Decomposition with Controlled Liquid Transport

The Energies of Activation and Deactivation of 2,4-Dichlorophenol Degradation by Horseradish Peroxidase Immobilized on the Modified Nanofibrous Membrane

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