Thermal Stability of Hepatitis E Virus

Emerson, Suzanne U.; Arankalle, Vidya A.; Purcell, Robert H.

doi:10.1086/432488

Cited by 192 publications

(152 citation statements)

References 11 publications

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“…The meat samples evaluated in this study were often destined for commercial ends, primarily for the production of sausages or processed meats (Williams et al, 2001). HEV particles remain infectious and can resist external and internal temperatures of under or badly cooked meat (Emerson et al, 2005;Feagins et al, 2008). Additionally, after slaughter, animal parts are kept in cooling chambers that may favor HEV viability in the organs and tissues of animals that are infected during slaughter.…”

Section: Discussionmentioning

confidence: 99%

Detection of hepatitis E virus genome in pig livers in Antioquia, Colombia

Gutiérrez-Vergara¹,

Quintero²,

Duarte³

et al. 2015

Genet. Mol. Res.

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Section: Discussionmentioning

confidence: 99%

Detection of hepatitis E virus genome in pig livers in Antioquia, Colombia

Gutiérrez-Vergara¹,

Quintero²,

Duarte³

et al. 2015

Genet. Mol. Res.

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“…Additional strong evidence for foodborne transmission is available from Japan where sequence identity between deer meat [104,105] and hepatitis E patients who consumed that meat, and HEV genetic similarity between boar meat and patient samples [106], have been found. HEV has been shown to be viable and infectious in pigs' liver in the USA [107] and resistant to temperatures up to 60 xC [108]. Consequently, the role of foodborne transmission in European countries other than Germany should be assessed through further analytical studies.…”

Section: Discussionmentioning

confidence: 99%

Transmission routes and risk factors for autochthonous hepatitis E virus infection in Europe: a systematic review

2009

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SUMMARYIncreasing numbers of non-travel-associated hepatitis E virus (HEV) infections have been reported in Europe in recent years. Our objective was to review the evidence on risk factors and transmission routes of autochthonous HEV infection and hepatitis E in Europe in order to develop recommendations for future research, prevention and control. A systematic literature review was performed to identify all primary reports and studies published during 1998-2008 on hepatitis E in humans and animals in Europe by searching Pubmed, reference lists of major articles and international conference proceedings. Each of the 106 included studies was categorized into one of three evidence levels (EL) based on study design and diagnostic methodology. The evidence was generally weak (73 were assigned to EL1, two to both EL1 and EL2, and 30 to EL2), further compounded by the use of poorly validated serological assays in some studies. Only one casecontrol study was assigned to EL3. Persons with autochthonous hepatitis E infection were on average older than the general population and predominantly male. There was no evidence for one main transmission route of HEV infection or risk factor for hepatitis E. However, zoonotic transmission seemed likely and person-to-person transmission too inefficient to cause clinical disease. Multiple routes of transmission probably exist and should be further investigated through analytical studies and reliable diagnostic kits. Based on current evidence that points to zoonotic transmission from pigs, thorough cooking of all porcine products, prevention of crosscontamination in the kitchen and improved education for occupationally exposed people (e.g. pig farmers, veterinarians and sewage workers) may help prevent HEV infection. Although evidence for parenteral transmission is limited, it is recommended that a risk assessment is undertaken.

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“…Previous findings indicate that HEV has a limited ability to infect and replicate in HepG2 cells (Emerson et al, 2005He et al, 2008). In blocking experiments, HepG2 cells were initially treated with HEV for 1 h at 4 uC.…”

Section: Methodsmentioning

confidence: 99%

“…He et al (2008) used p239 to simulate native HEV to investigate virus attachment on hepatocytes. It has also been shown that p239 attached and entered the cells of four susceptible cell lines, HepG2 (Emerson et al, 2004(Emerson et al, , 2005Graff et al, 2008), Huh7 (Emerson et al, 2004(Emerson et al, , 2005Graff et al, 2008), PLC/PRF5 (Meng et al, 1997;Tanaka et al, 2007) and A549 (Huang et al, 1995;Tanaka et al, 2007), and binding was blocked by neutralizing monoclonal antibodies (mAbs). Moreover, the interaction between p239 and the host cells was dependent on the dimeric conformation fit of p239 and the binding significantly inhibited HEV infectivity on HepG2 and Huh7 cells.…”

Section: Introductionmentioning

confidence: 99%

Role of heat-shock protein 90 in hepatitis E virus capsid trafficking

Zheng

Jiang

Zhao

et al. 2010

Journal of General Virology

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p239 is a virus-like particle constituted from hepatitis E virus (HEV) recombinant proteins. It can be used as a surrogate for HEV and as an investigative tool to study cellular interactions because of its ability to adsorb to and penetrate HepG2 cellular membranes. Our objective was to use p239 to define the role of HEV capsid proteins during the early stages of infection. Pull-down and MALDI-TOF MS experiments identified three host-cell proteins, Grp 78/Bip, a-tubulin and heatshock protein 90 (HSP90), and the latter was investigated further. Antibodies to p239 alone or HSP90 alone could detect p239 or HSP90, suggesting the formation of a complex between p239 and HSP90. In the HepG2 cell, geldanamycin (GA), an HSP90-specific inhibitor, blocked intracellular transportation of p239, but had no effect on the binding and cellular entry of p239, suggesting that HSP90 was important for HEV capsid intracellular transportation. RT-PCR results showed that the efficiency of wild-type HEV infection was inhibited significantly by GA treatment, suggesting the importance of HSP90 in virus infectivity. It was concluded that HSP90 plays a crucial role in the intracellular transportation of viral capsids in the early stage of HEV infection.

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Thermal Stability of Hepatitis E Virus

Cited by 192 publications

References 11 publications

Detection of hepatitis E virus genome in pig livers in Antioquia, Colombia

Detection of hepatitis E virus genome in pig livers in Antioquia, Colombia

Transmission routes and risk factors for autochthonous hepatitis E virus infection in Europe: a systematic review

Role of heat-shock protein 90 in hepatitis E virus capsid trafficking

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