Thermal stability of the multiple charge isoforms of inulase fromAspergillus niger

Fermentation of Inulin by Clostridium thermosuccinogenes sp. nov., a Thermophilic Anaerobic Bacterium Isolated from Various Habitats

“…The temperature optimum of 58°C and the thermostability at up to 60°C for the inulinases produced by C. thermosuccinogenes are among the highest reported thus far (49). The enzymes of strain IC are more thermostable than those of the biotechnologically important yeast Kluyveromyces marxianus (37) and are comparable to the inulinases which have been isolated from Aspergillus niger (48). Unfortunately, the total activity of the inulinases present at the end of growth in a batch culture of strain IC is very low (6.9 U g of inulin degraded-1 liter-') compared with that of yeasts (21,200 U g of inulin degraded-' liter-' [33]).…”

Thermal stability of an acidic inulinase from Scytalidium acidophilum

“…It is interesting that S. acidopbilum, which was isolated from very low acidic media (Starkey and Waksman, 1943), can produce a thermostable exoinulinase. Among the inulinases, only a thermostable inulinase from A. niger (Uhm et al, 1987) could maintain 42 ~ of its original activity after 6 h of incubation at 65 * C. So far, in view of thermal stability, inulinase from S. acidopbilum seems to be the first candidate which has the industrial potential for inulin hydrolysis. Further investigations are in process to elucidate the properties of this enzyme.…”

Fermentation of inulin by a new strain of Clostridium thermoautotrophicum isolated from dahlia tubers

“…The thermostability and temperature optimum of the inulinases produced by strain I1 belong to the highest described in the literature so far [2]. The enzymes of strain 11 are more thermostable than the biotechnologically important yeast Kluyveromyces marxianus [4], and comparable to the inulinases, which have been obtained from Aspergillus niger [6]. At 60°C the inulinase activity of K. marxianus was reduced by 50% after 30 min of incubation, whereas no loss of inulinase-activity of strain I1 and Aspergillus niger could be detected after three h of incubation.…”

“…Therefore we have chosen this relatively abundant, biotechnologically promising polysaccharide as a model substrate to study polymer hydrolysing fermentative bacteria both in pure and in mixed culture. Especially thermostable inulinases could find application in the production of high fructose syrups because the poor solubility of inulin at low temperature is the limiting factor in obtaining high conversion ratios [6].…”