Thermal sterilization of heat‐sensitive products using high‐temperature short‐time sterilization

Mann, A. C.; Kiefer, Markus; Leuenberger, Hans

doi:10.1002/1520-6017(200103)90:3<275::aid-jps3>3.0.co;2-i

Cited by 40 publications

(28 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Antibiotics, for example, have come to be commonly used to prevent diseases due to seafood in fishery and culture, but chemical disinfectants may be toxic, deteriorate with an abnormal smell, and cause increased resistance to antibiotics (8). Heat sterilization is not suitable for large-scale treatment and culturing of fish (12). Ozone and UV light are efficient and produce harmless derivatives but are more costly (3,5,19).…”

supporting

confidence: 81%

Inactivation of Bacteria in Seawater by Low-Amperage Electric Current

Park

Lee

et al. 2003

Appl Environ Microbiol

View full text Add to dashboard Cite

Seawater used in mariculture has been suspected of being a potential source of infection. In this study, the lethal effects of low-amperage electric treatment on microorganisms were examined in natural seawater and in seawater inoculated with Vibrio parahaemolyticus. In both cases, bacteria including V. parahaemolyticus in seawater were completely eliminated in 100 ms by a 0.5-A, 12-V direct current. Electron microscopic investigation of the electrically treated bacteria revealed substantial structural damage at the cellular level. In conclusion, our results indicate that low-amperage electric treatment is effective for rapid inactivation of microorganisms in seawater.The prevalence and distribution of bacteria such as Vibrio species in aquatic environments are of great public health concern because Vibrio spp. are pathogenic for both humans and animals (18). Recent outbreaks of Vibrio parahaemolyticus in the United States have been associated with eating behavior such as the consumption of raw or undercooked seafood, including fish and shellfish (1, 4, 9). Hence, seawater used for the cultivation and washing of fish and shellfish is another potential source of infection. However, conventional techniques of water sterilization are typically unsuitable for seawater. Antibiotics, for example, have come to be commonly used to prevent diseases due to seafood in fishery and culture, but chemical disinfectants may be toxic, deteriorate with an abnormal smell, and cause increased resistance to antibiotics (8). Heat sterilization is not suitable for large-scale treatment and culturing of fish (12). Ozone and UV light are efficient and produce harmless derivatives but are more costly (3,5,19). Recently, the use of pulsed electric fields, which are nonthermal and nonchemical, has been researched for sterilization of water (13,16,21,22). However, the industrial plant required for such treatment represents a major investment for manufacturers because of the limited application of high-voltage electric fields. We also have studied an electric sterilization method for seawater that is based on low-amperage direct-current transfer between bacterial cells and electrodes rather than on the generation of toxic substrates such as H 2 O 2 (10). In contrast to a pulsed electric field, the method described here uses a lowintensity electric current and is therefore potentially much simpler and cheaper to implement. In the present study, the sterilizing effect of low-amperage electric treatment for various times was studied with natural seawater and seawater inoculated with V. parahaemolyticus. Because V. parahaemolyticus is known to cause gastrointestinal illness in humans, it seemed to be suitable as our experimental model.The natural seawater used in this study was collected in the seashore of In-cheon in Korea. V. parahaemolyticus (ATCC 17802; Shirasu food-poisoning isolate) was grown in nutrient broth (Difco Laboratories, Detroit, Mich.) containing 3% (wt/ vol) NaCl at 37°C. At the end of the exponential growth phase, the cells we...

show abstract

supporting

confidence: 81%

Inactivation of Bacteria in Seawater by Low-Amperage Electric Current

Park

Lee

et al. 2003

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…Aseptic processing in a clean room can be used to manufacture germless terminal products but sterilization of final products is preferred due to aspect of economy and convenience. The common sterilization methods are ethylene oxide (EO) gas, 19 dry heat, 20 steam, 21 organic solvent (ethanol), 22 plasma treatment, 23 and gamma irradiation. 24 Among these, sterilization method using EO gas has the residual possibility of EO showing fatal toxicity.…”

Section: Introductionmentioning

confidence: 99%

The effect of gamma irradiation on PLGA and release behavior of BCNU from PLGA wafer

et al. 2003

View full text Add to dashboard Cite

The objectives of this study were to investigate the influence of gamma irradiation for sterilization on poly(D,L-lactide-co-glycolide) (PLGA) with different molecular weight and the effect of gamma irradiation on the release behavior of 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU, carmustine) from PLGA wafer with various irradiation doses. The effect of gamma irradiation on PLGA was evaluated by gel permeation chromatography (GPC), differential scanning calorimetry (DSC), and electron paramagnetic resonance (EPR). The weight average molecular weight (M w ) and glass transition temperature (T g ) of PLGA decreased after gamma irradiation. The extent of M w reduction was dependent on irradiation dose and PLGA molecular weight. Using EPR spectroscopy, we successfully detected gamma irradiation induced free radicals in PLGA. The gamma irradiation increased the release rate of BCNU from PLGA wafer at applied irradiation doses except 2.5 Mrad of irradiation dose in this study.

show abstract

“…Heat inactivation is not suitable for large-scale treatment and culturing of fish 22 . Numerous highpressure carbon dioxide or hydrostatic pressure inactivation of bacterial cells are potentially available 23 , but seems to be difficult to apply to food industry and have little inactivation effects on the bacterial cells 24 .…”

Section: Introductionmentioning

confidence: 99%

Comparison of the Efficacy of Selected Bacterins against Edwardsiella Tarda in Immunized Japanese eel (Anguilla japonica)

Hossain

Kawai

Duston

et al. 2016

Bangla. J. Microbiol.

View full text Add to dashboard Cite

An effective vaccine against Edwardsiella tarda has not been reported in substitution for high concentration of formalin for the prevention of edwardsiellosis disease. In this study, the efficacy of inactivated E. tarda was evaluated and compared by intraperitoneal (IP) injection-immunization or challenge against Japanese eel (Anguilla japonica). Formalin, formalin with heat, citric acid, pressure and electric current were used for inactivation of the bacteria, and the relative percent survival (RPS) values of pressure (600 psi for 5 min) killed cells was determined. PKCinactivated vaccine showed 89-93% protection that was higher than others. PKC-inactivated vaccine at a concentration of 10 6 cells/fish was sufficient to induce high protection (RPS>89). Protection of the different-inactivated vaccines was evaluated at different time post immunization, and the peak of protection was observed at 9 days post-challenge. Fish immunized with PKC showed significantly (P<0.05) higher serum and mucus antibody titers elicit both systemic and mucosal adaptive immune responses, and induce specific humoral immune responses in eel. Coincident with higher protection, sera of fish immunized with the PKC vaccine had higher agglutination titers than FKC, FHKC, CAKC and ECKC. All these data strongly suggested that PKC vaccine is an effective strategy to protect eel against edwardsiellosis.

show abstract

Thermal sterilization of heat‐sensitive products using high‐temperature short‐time sterilization

Cited by 40 publications

References 11 publications

Inactivation of Bacteria in Seawater by Low-Amperage Electric Current

Inactivation of Bacteria in Seawater by Low-Amperage Electric Current

The effect of gamma irradiation on PLGA and release behavior of BCNU from PLGA wafer

Comparison of the Efficacy of Selected Bacterins against Edwardsiella Tarda in Immunized Japanese eel (Anguilla japonica)

Contact Info

Product

Resources

About