Thermal stress responses in Antarctic yeast, Glaciozyma antarctica PI12, characterized by real-time quantitative PCR

Boo, Sook Yee; Wong, Clemente Michael Vui Ling; Rodrigues, Kenneth Francis; Najimudin, Nazalan; Murad, Abdul Munir Abdul; Mahadi, Nor Muhammad

doi:10.1007/s00300-012-1268-2

Cited by 51 publications

(38 citation statements)

References 66 publications

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“…7). Similar data were reported that cold exposure significantly increased activities of superoxide dismutase (SOD) in Antarctic plants (Arora et al 2002), algae (Collén and Davison 2001), and animals (Ansaldo et al 2000;Abele et al 2001;Heise et al 2006;Boo et al 2013). The major reasons hypothesized for this phenomenon were that plants subjected to temperature stress contribute to start their own antioxidant defense mechanism by adjusting the level of expression of the antioxidant enzyme gene.…”

Section: Discussionsupporting

confidence: 80%

cDNA cloning, characterization and expression analysis of manganese superoxide dismutase in Ulva prolifera

Fan

Sun

et al. 2015

J Appl Phycol

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Superoxide dismutase (SOD) is an important antioxidant protein that protects organisms against various oxidative stresses by eliminating hydrogen peroxide. In the present study, a full-length cDNA sequence of manganese superoxide dismutase (MnSOD) from the Ulva prolifera (denoted as UpMnSOD) was cloned by a reverse-transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA end method. The UpMnSOD cDNA sequence contained 955 bp with an open reading frame of 699 bp encoding 232 amino acid residues. The cDNA contained a 5′-untranslated region (UTR) of 29 bp nucleotides and a long 3′-UTR of 227 bp nucleotides. The calculated molecular mass was 25.7 kDa, and the estimated isoelectronic point (pI) of this protein was 6.83. Histidine (His) 59, His103, His194, and aspartate (Asp) 190 were found to be activated sites in UpMnSOD. Multiple alignment analysis revealed that the deduced amino acid sequence of MnSOD shared high identity (86 %) with Ulva fasciata. A phylogenetic tree construct indicated that UpMnSOD clustered into one subgroup with the MnSOD from U. fasciata. Quantitative real-time analysis revealed that UpMnSOD expression was induced at both 35 and 5°C. The result indicated that UpMnSOD expression levels reached a maximum point of 2.63-fold (P<0.05) and 1.28-fold compared to the control at 25°C after 6-h treatment at 35 and 5°C, respectively, and that long-time heat shock and cold treatment decreased the mRNA expression levels of MnSOD. Furthermore, UpMnSOD expression increased after 15°C treatment, a 2.4-fold increase after 12-h treatment. Treatment with 1 mmol·L −1 salicylic acid (SA) at 35°C also increased the gene expression of UpMnSOD and reached a maximum of 1.48-fold of the control after 6-h treatment, followed by a gradual decrease. However, the gene expression level of UpMnSOD increased rapidly within 3 h then decreased quickly under SA 5 mmol·L −1 + 35°C. These data indicate that MnSOD was perhaps involved in the acute response against temperature stress, and salicylic acid could alleviate the high temperature stress effect on U. prolifera.

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Section: Discussionsupporting

confidence: 80%

cDNA cloning, characterization and expression analysis of manganese superoxide dismutase in Ulva prolifera

Fan

Sun

et al. 2015

J Appl Phycol

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“…The culturing of Glyciozyma antarctica PI12 was conducted in collaboration with the Malaysia Genome Institute [11]. The psychrophilic yeast, Glyciozyma antarctica PI12 was originally isolated from Casey sea ice research station located in Antarctica.…”

Section: Culturing Materialsmentioning

confidence: 99%

NEW INDOLIZIDINE ALKALOIDS FROM PSYCHROPHILIC YEAST Glyciozyma antarctica PI12

Rosandy

Nadarajah

Bakar³

et al. 2016

MJAS

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“…Glaciozyma antarctica strain PI12 has an optimum growth temperature of 12°C [14] and can grow up to 18°C. The reclassification of this yeast from L. antarcticum to G. antarctica was proposed by Turchetti et al, 2011 [15].…”

Section: Introductionmentioning

confidence: 99%

Molecular Cloning and Optimization for High Level Expression of Cold-Adapted Serine Protease from Antarctic Yeast Glaciozyma antarctica PI12

et al. 2014

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Psychrophilic basidiomycete yeast, Glaciozyma antarctica strain PI12, was shown to be a protease-producer. Isolation of the PI12 protease gene from genomic and mRNA sequences allowed determination of 19 exons and 18 introns. Full-length cDNA of PI12 protease gene was amplified by rapid amplification of cDNA ends (RACE) strategy with an open reading frame (ORF) of 2892 bp, coded for 963 amino acids. PI12 protease showed low homology with the subtilisin-like protease from fungus Rhodosporidium toruloides (42% identity) and no homology to other psychrophilic proteases. The gene encoding mature PI12 protease was cloned into Pichia pastoris expression vector, pPIC9, and positioned under the induction of methanol-alcohol oxidase (AOX) promoter. The recombinant PI12 protease was efficiently secreted into the culture medium driven by the Saccharomyces cerevisiae α-factor signal sequence. The highest protease production (28.3 U/ml) was obtained from P. pastoris GS115 host (GpPro2) at 20°C after 72 hours of postinduction time with 0.5% (v/v) of methanol inducer. The expressed protein was detected by SDS-PAGE and activity staining with a molecular weight of 99 kDa.

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Thermal stress responses in Antarctic yeast, Glaciozyma antarctica PI12, characterized by real-time quantitative PCR

Cited by 51 publications

References 66 publications

cDNA cloning, characterization and expression analysis of manganese superoxide dismutase in Ulva prolifera

cDNA cloning, characterization and expression analysis of manganese superoxide dismutase in Ulva prolifera

NEW INDOLIZIDINE ALKALOIDS FROM PSYCHROPHILIC YEAST Glyciozyma antarctica PI12

Molecular Cloning and Optimization for High Level Expression of Cold-Adapted Serine Protease from Antarctic Yeast Glaciozyma antarctica PI12

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