Thermally stable improved first-generation glucose biosensors based on Nafion/glucose-oxidase modified heated electrodes

Tseng, Ta-Feng; Yang, Yang-Li; Chuang, Min‐Chieh; Lou, Shyh-Liang; Galík, Michal; Flechsig, Gerd‐Uwe; Wang, Joseph

doi:10.1016/j.elecom.2009.07.030

Cited by 29 publications

(15 citation statements)

References 15 publications

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“…The main advantages of first-generation amperometric biosensors are their high sensitivity and the low response time (around one second). However, drawbacks such as the requirement of coenzymes and the high potential for operation, fouling transducers surface due to prolonged use, and an error that relies on electron acceptor concentration (such as dissolved molecular oxygen concentration) may limit its usage in biological systems [18,19,20]. In order to eliminate oxygen dependency, second-generation biosensors use a mediator (electron acceptor), instead of oxygen, to transport the electrons to the electrode.…”

Section: Enzyme-based Biosensorsmentioning

confidence: 99%

Immobilized Enzymes in Biosensor Applications

et al. 2019

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Enzyme-based biosensing devices have been extensively developed over the last few decades, and have proven to be innovative techniques in the qualitative and quantitative analysis of a variety of target substrates over a wide range of applications. Distinct advantages that enzyme-based biosensors provide, such as high sensitivity and specificity, portability, cost-effectiveness, and the possibilities for miniaturization and point-of-care diagnostic testing make them more and more attractive for research focused on clinical analysis, food safety control, or disease monitoring purposes. Therefore, this review article investigates the operating principle of enzymatic biosensors utilizing electrochemical, optical, thermistor, and piezoelectric measurement techniques and their applications in the literature, as well as approaches in improving the use of enzymes for biosensors.

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Section: Enzyme-based Biosensorsmentioning

confidence: 99%

Immobilized Enzymes in Biosensor Applications

et al. 2019

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“…The electrodes are modified with the necessary enzymatic machinery for the specificd etection cascades to retain enzymatic activity,i ncrease the electroactive area and diminish undesireda dsorption. [57,58] This concept utilizes the detection of H 2 O 2 by biocatalytic action of HRP directly on the electrode surface, allowing for quantitative detection of H 2 O 2 .…”

Section: Discussion and Summarymentioning

confidence: 99%

Promises and Challenges in Continuous Tracking Utilizing Amino Acids in Skin Secretions for Active Multi‐Factor Biometric Authentication for Cybersecurity

2017

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We consider a new concept of biometric‐based cybersecurity systems for active authentication by continuous tracking, which utilizes biochemical processing of metabolites present in skin secretions. Skin secretions contain a large number of metabolites and small molecules that can be targeted for analysis. Here we argue that amino acids found in sweat can be exploited for the establishment of an amino acid profile capable of identifying an individual user of a mobile or wearable device. Individual and combinations of amino acids processed by biocatalytic cascades yield physical (optical or electronic) signals, providing a time‐series of several outputs that, in their entirety, should suffice to authenticate a specific user based on standard statistical criteria. Initial results, motivated by biometrics, indicate that single amino acid levels can provide analog signals that vary according to the individual donor, albeit with limited resolution versus noise. However, some such assays offer digital separation (into well‐defined ranges of values) according to groups such as age, biological sex, race, and physiological state of the individual. Multi‐input biocatalytic cascades that handle several amino acid signals to yield a single digital‐type output, as well as continuous‐tracking time‐series data rather than a single‐instance sample, should enable active authentication at the level of an individual.

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“…Practical limitations, however, prevent measurements at the critical state of the used solution [40,41]. In a simplified version called "temperature pulse amperometry" (TPA), we proposed application of heat pulses to modulate amperometric signals in order to obtain peak-shaped signals, which can also compensate a shifting background [34,42,43].…”

Section: Introductionmentioning

confidence: 99%

Directly Heated Bismuth Film Electrodes Based on Gold Microwires

et al. 2010

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As a nontoxic substitute for mercury electrodes, bismuth electrodes attained a lot of attention during the last years. In this report we describe for the first time the preparation of two different directly heatable bismuth-modified microwire electrodes. We characterized the electrochemical behaviour using cyclic voltammetry in acetate buffer and alkaline tartrate solution. The bismuth electrodes show a significantly wider potential window compared with bare gold wires. In the presence of picric acid as one example for the detection of explosives, the bismuth electrodes deliver higher signals. By applying heat during the measurements, the signals can be enhanced further. We used the temperature pulse amperometry (TPA) technique to improve the electrochemical response at the different types of electrodes. In this preliminary study, we were able to detect 3 ppm traces of picric acid.

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Thermally stable improved first-generation glucose biosensors based on Nafion/glucose-oxidase modified heated electrodes

Cited by 29 publications

References 15 publications

Immobilized Enzymes in Biosensor Applications

Immobilized Enzymes in Biosensor Applications

Promises and Challenges in Continuous Tracking Utilizing Amino Acids in Skin Secretions for Active Multi‐Factor Biometric Authentication for Cybersecurity

Directly Heated Bismuth Film Electrodes Based on Gold Microwires

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