Thermally Switchable Nanogate Based on Polymer Phase Transition

Abstract

Mimicking and extending the gating properties of biological pores is of paramount interest for the fabrication of membranes that could be used in filtration or drug processing. Here, we build a selective and switchable nanopore for macromolecular cargo transport. Our approach exploits polymer graftings within artificial nanopores to control the translocation of biomolecules. To measure transport at the scale of individual biomolecules, we use fluorescence microscopy with a zero-mode waveguide set up. We show that grafting polymers that exhibit a lower critical solution temperature creates a toggle switch between an open and closed state of the nanopore depending on the temperature. We demonstrate tight control over the transport of DNA and viral capsids with a sharp transition (∼1 °C) and present a simple physical model that predicts key features of this transition. Our approach provides the potential for controllable and responsive nanopores in a range of applications.

Optical single molecule characterisation of natural and synthetic polymers through nanopores

“…The zero-mode waveguide for the nanopore technique has been used previously to study the transport of biomolecules and viral particles. 7,11,14–17 In this work, we developed a series of tools to characterise the translocation of a polymer through a nanopore. In particular, we measure the characteristic time of a translocation event.…”