Thermic phosphorylations. V. Fractionation of products of thermic reaction of uridylic acid with uridine using gel filtration and ion-exchange chromatography

Alternating co-synthesis of glycol nucleic acid (GNA) monomers with dicarboxylic acids via drying

“…However, phosphodiester linkage formation is thermodynamically unfavorable without chemical activation, 9 i.e., the most feasible way to synthesize RNA without chemical activation is under dry-down conditions, which are often invoked in prebiotic chemical syntheses. [10][11][12][13] RNA polymerization by dry-down under neat circumstances is generally inefficient 14 and requires specialized conditions, e.g., salt-promoted 15 or lipidassisted synthesis, 16 or templating by minerals. 17 However, in realistic prebiotic scenarios, drying environments likely contained more than just pure ribonucleotides and lipids.…”

“…, the most feasible way to synthesize RNA without chemical activation is under dry-down conditions, which are often invoked in prebiotic chemical syntheses. 10–13 RNA polymerization by dry-down under neat circumstances is generally inefficient 14 and requires specialized conditions, e.g. , salt-promoted 15 or lipid-assisted synthesis, 16 or templating by minerals.…”