Thermo-/pH-responsive chitosan-cellulose nanocrystals based hydrogel with tunable mechanical properties for tissue regeneration applications

Maturavongsadit, Panita; Paravyan, Gayane; Shrivastava, Roopali; Benhabbour, S. Rahima

doi:10.1016/j.mtla.2020.100681

Cited by 38 publications

(55 citation statements)

References 81 publications

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“…This system is thermo-sensitive and can form hydrogels instantly within 7 s at 37 °C through a mechanism that involves hydrophobic interactions within the chitosan network and hydrogen bonding between chitosan and CNCs. We demonstrated that the CNCs-incorporated chitosan-based formulations were biocompatible, supported cell encapsulation and viability, and were injectable through a 20 G needle without compromising cell viability . Importantly, CNCs had a significant impact on the mechanical properties of the hydrogel and on the differentiation and behavior of pre-osteoblasts encapsulated within the hydrogel.…”

Section: Introductionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

“…We recently developed a tunable thermo-/pH-responsive chitosan hydrogel incorporated with cellulose nanocrystals (CNCs) as an injectable biomaterial for the encapsulation of pre-osteoblasts (MC3T3-E1 cells) for minor bone fracture repair . Chitosan has been widely used in bone tissue engineering applications. − In our previous injectable system, the formulation was prepared using chitosan, CNCs, β-glycerophosphate, and hydroxyethyl cellulose (HEC) . This system is thermo-sensitive and can form hydrogels instantly within 7 s at 37 °C through a mechanism that involves hydrophobic interactions within the chitosan network and hydrogen bonding between chitosan and CNCs.…”

Section: Introductionmentioning

confidence: 99%

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Cell-Laden Nanocellulose/Chitosan-Based Bioinks for 3D Bioprinting and Enhanced Osteogenic Cell Differentiation

Maturavongsadit

Narayanan

Chansoria

et al. 2021

ACS Appl. Bio Mater.

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3D bioprinting has recently emerged as a very useful tool in tissue engineering and regenerative medicine. However, developing suitable bioinks to fabricate specific tissue constructs remains a challenging task. Herein, we report on a nanocellulose/chitosan-based bioink, which is compatible with a 3D extrusion-based bioprinting technology, to design and engineer constructs for bone tissue engineering and regeneration applications. Bioinks were prepared using thermogelling chitosan, glycerophosphate, hydroxyethyl cellulose, and cellulose nanocrystals (CNCs). Formulations were optimized by varying the concentrations of glycerophosphate (80−300 mM), hydroxyethyl cellulose (0−0.5 mg/mL), and CNCs (0−2% w/v) to promote fast gelation kinetics (<7 s) at 37 °C and retain the shape integrity of constructs post 3D bioprinting. We investigated the effect of CNCs and pre-osteoblast cells (MC3T3-E1) on the rheological properties of bioinks, bioink printability, and mechanical properties of bioprinted scaffolds. We demonstrate that the addition of CNCs and cells (5 million cells/mL) significantly improved the viscosity of bioinks and the mechanical properties of chitosan scaffolds post-fabrication. The bioinks were biocompatible and printable at an optimized range of printing pressures (12−20 kPa) that did not compromise cell viability. The presence of CNCs promoted greater osteogenesis of MC3T3-E1 cells in chitosan scaffolds as shown by the upregulation of alkaline phosphatase activity, higher calcium mineralization, and extracellular matrix formation. The versatility of this CNCs-incorporated chitosan hydrogel makes it attractive as a bioink for 3D bioprinting to engineer scaffolds for bone tissue engineering and other therapeutic applications.

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Section: Introductionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cell-Laden Nanocellulose/Chitosan-Based Bioinks for 3D Bioprinting and Enhanced Osteogenic Cell Differentiation

Maturavongsadit

Narayanan

Chansoria

et al. 2021

ACS Appl. Bio Mater.

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“…With a safe and stable structural color, CNCs can be modified or combined with other compounds to prepare light, heat, pH, humidity, or gas sensitive films. [30][31][32][33][34][35] The selection of compounds is one of the determinants to prepare highquality CNC films. Polyethylene glycol has been proven to be a kind of effective humidity sensitive compound, while high molecular weight polymers like polyethylene glycol are prone to destroy the layered structure of CNC films.…”

Section: Introductionmentioning

confidence: 99%

“…Many studies have shown that by changing the magnetic field intensity, 20 the starting concentration, 21 the solvent, 22,23 the evaporation rate, 24 the evaporation temperature, 25 the particle size, 26,27 the surface charge, 28 and the ionic strength of the liquid, 29 the pitch can be manipulated to adjust the structure color of the film. With a safe and stable structural color, CNCs can be modified or combined with other compounds to prepare light, heat, pH, humidity, or gas sensitive films 30–35 . The selection of compounds is one of the determinants to prepare high‐quality CNC films.…”

Section: Introductionmentioning

confidence: 99%

The preparation of cellulose nanocrystal/1,3‐butylene glycol composite structural color films and humidity‐responsive

Wang

Meng

et al. 2022

J of Applied Polymer Sci

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Cellulose nanocrystals (CNCs) can self‐assemble in an aqueous solution to form a chiral nematic liquid crystal structure, which is maintained in the subsequent solid iridescent film and presents a unique structural color. In this study, in order to explore the effect of ultrasound on the optical properties of CNC, CNC films with a chiral nematic helix structure and different structural colors were prepared by ultrasonic‐assisted dispersion and natural static evaporation. Results showed that prolonging the ultrasonic duration significantly increased layer pitches and red‐shifted the color of CNC films, likely due to increased steric hindrance and a larger electric double layer. On this basis, CNC/1,3‐butylene glycol (BG) composite films were prepared with uniform and structural color. Results showed that BG was incorporated into CNCs most likely through hydrogen bonds. Moreover, the presence of BG increased the layer pitches and red‐shifted the structural color of CNC/BG composite films, which indicates that structural color of the film has a good stability response to humidity. On the whole, our work provides a new point of view in understanding the role of ultrasound and small‐molecule additives in the preparation of CNC‐based films.

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Rapid Synthesis of Functions‐Integrated Hydrogel as a Self‐Powered Wound Dressing for Real‐Time Drug Release and Health Monitoring

Li,

Zhang,

Peng

et al. 2024

Adv Healthcare Materials

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A bio‐hydrogel is prepared via a low‐cost and time‐saving strategy and is studied as a self‐powered wound dressing for precision medicine and health monitoring. Promoted by a dual self‐catalytic pair composed of Fe3+ and catechol, gelation time is dramatically accelerated to 15 s and the hydrogel can be freely modeled at −18 °C without losing flexibility. As smart wound dressing, the required properties such as self‐healing, self‐adhesion, antibacterial, and sensing stability, are integrated into one hydrogel. TA@CNC offers abundant hydrogen bond and metal‐ligand coordination which facilitate the hydrogel with a self‐healing efficiency of 91.6%. Owing to the catechol in TA@CNC, hydrogel can adhere to multiple substrates including skin, and show good antibacterial activity. Inspired by a fruit battery, a self‐powered wound dressing is fabricated, which exhibits excellent correlation and efficiency in real‐time monitoring of body activity and drug release. In vivo experiments prove that efficient drug release of hydrogel dressing significantly accelerate wound healing. Additionally, the dressing exhibits excellent biocompatibility and has no negative impacts on organs. Herein, a smart wound dressing that is different from the traditional way is proposed. As a self‐powered device, it can be integrated with wireless devices and is expected to participate in promising applications.

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Thermo-/pH-responsive chitosan-cellulose nanocrystals based hydrogel with tunable mechanical properties for tissue regeneration applications

Cited by 38 publications

References 81 publications

Cell-Laden Nanocellulose/Chitosan-Based Bioinks for 3D Bioprinting and Enhanced Osteogenic Cell Differentiation

Cell-Laden Nanocellulose/Chitosan-Based Bioinks for 3D Bioprinting and Enhanced Osteogenic Cell Differentiation

The preparation of cellulose nanocrystal/1,3‐butylene glycol composite structural color films and humidity‐responsive

Rapid Synthesis of Functions‐Integrated Hydrogel as a Self‐Powered Wound Dressing for Real‐Time Drug Release and Health Monitoring

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