Thermo-responsive imprinted hydrogel with switchable sialic acid recognition for selective cancer cell isolation from blood

Yin, Yimei; Ni, Li; Miao, Haohan; Wang, Yingjia; Pan, Cheng; Tian, Xiaohua; Pan, Jianming; You, Tianyan; Li, Bin; Pan, Guoqing

doi:10.1016/j.bioactmat.2020.10.008

Cited by 46 publications

(25 citation statements)

References 45 publications

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“…The thermo-responsive hydrogel (NIPAAm) allowed to tune the capture and release of cells; at 37 °C, the binding sites are all exposed and cancer cells can bind to them, while at temperatures lower than 25 °C the sites change in shape and functionality, so that the cells may be quickly released. Results showed the selective capture of cancer cells (HepG-2) and release by changing the temperature [83]. The SA epitope is imprinted over the surface of the PNIPAAm hydrogel; at 37 °C, the epitope is exposed on the surface, so that cancer cells can bind to it.…”

Section: Epitope Imprintingmentioning

confidence: 99%

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Molecular Imprinting Strategies for Tissue Engineering Applications: A Review

2021

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Tissue Engineering (TE) represents a promising solution to fabricate engineered constructs able to restore tissue damage after implantation. In the classic TE approach, biomaterials are used alongside growth factors to create a scaffolding structure that supports cells during the construct maturation. A current challenge in TE is the creation of engineered constructs able to mimic the complex microenvironment found in the natural tissue, so as to promote and guide cell migration, proliferation, and differentiation. In this context, the introduction inside the scaffold of molecularly imprinted polymers (MIPs)—synthetic receptors able to reversibly bind to biomolecules—holds great promise to enhance the scaffold-cell interaction. In this review, we analyze the main strategies that have been used for MIP design and fabrication with a particular focus on biomedical research. Furthermore, to highlight the potential of MIPs for scaffold-based TE, we present recent examples on how MIPs have been used in TE to introduce biophysical cues as well as for drug delivery and sequestering.

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Section: Epitope Imprintingmentioning

confidence: 99%

“…Finally, in ( d ) the staining of the cell on the hydrogel surface at 37 °C (captured cells) and 25 °C (released cells). Figure modified with permission from [ 83 ].…”

Section: Figurementioning

confidence: 99%

Molecular Imprinting Strategies for Tissue Engineering Applications: A Review

2021

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“…F I G U R E 3 Schematic illustration for the synthesis of molecularly imprinted polymer nanoparticles that specifically target a cell membrane protein [44] F I G U R E 4 (A) Schematic illustration for the imaging of sialic acid (SA) terminated glycan motifs using MIPs. (B) The surface grafting of an SA-imprinted shell on silica core particles mediated by reversible addition fragmentation chain transfer polymerization using a mixed covalent and noncovalent approach based on reversible amine catalyzed boronate esterification (1,3), hydrogen bond stabilization through a guest-responsive fluorescent reporter group (2), and electrostatic stabilization (3) [56] F I G U R E 5 Schematic illustration of targeting and imaging of cancer cells using monosaccharide-imprinted nanoparticles [62] Later, Pan and co-workers constructed an SA-imprinted thermo-responsive hydrogel layer to attain the selective capture and release of cancer cells triggered by changing temperature [57]. The generated SA-imprinted hydrogel was able to effectively capture cancer cells from the red blood samples.…”

Section: Polysaccharidementioning

confidence: 99%

Selective recognition of tumor cells by molecularly imprinted polymers

Gao

et al. 2021

J of Separation Science

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Molecularly imprinted polymers, developed 50 years ago, have garnered enormous attention as receptor-like materials. Lately, molecularly imprinted polymers have been employed as a specific target tool in favor of cancer diagnosis and therapy by the selective recognition of tumor cells. Although the molecular imprinting technology has been well-innovated recently, the cell still remains the most challenging target for imprinting. In this review, we summarize the advances in the synthesis of molecularly imprinted polymers suitable for the selective recognition of tumor cells. Through a sustained effort, three strategies have been developed including peptide-imprinting, polysaccharide-imprinting, and whole-cell imprinting, which have resulted in inspiring applications in effective cancer diagnosis and therapy. The major challenges and perspectives on the further directions related to the synthesis of molecularly imprinted polymers were also outlined.

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“…Due to the tight correlation between human health and environmental pollution that results in various diseases, environmental monitoring of pollutants and biomedical diagnosis of biomarkers are global concerns [ 1 , 2 ]. Hence, there is an increasing demand for developing sensors for the selective detection of various targets, such as abused pesticides and overproduced mycotoxins in food samples [ 3 , 4 , 5 , 6 ], as well as biomarkers and tumor cells that represent related diseases [ 7 , 8 , 9 , 10 ]. Moreover, the obtained sensors should be fabricated cost-effectively and capable of rapid, sensitive, portable applications.…”

Section: Introductionmentioning

confidence: 99%

Typical Fluorescent Sensors Exploiting Molecularly Imprinted Hydrogels for Environmentally and Medicinally Important Analytes Detection

Zou

Ding

et al. 2021

Gels

Self Cite

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In this work, two typical fluorescent sensors were generated by exploiting molecularly imprinted polymeric hydrogels (MIPGs) for zearalenone (ZON) and glucuronic acid (GA) detection, via the analyte’s self-fluorescence property and receptor’s fluorescence effect, respectively. Though significant advances have been achieved on MIPG-fluorescent sensors endowed with superior stability over natural receptor-sensors, there is an increasing demand for developing sensing devices with cost-effective, easy-to-use, portable advantages in terms of commercialization. Zooming in on the commercial potential of MIPG-fluorescent sensors, the MIPG_ZON is synthesized using zearalanone (an analogue of ZON) as template, which exhibits good detection performance even in corn samples with a limit of detection of 1.6 μM. In parallel, fluorescein-incorporated MIPG_GA is obtained and directly used for cancer cell imaging, with significant specificity and selectivity. Last but not least, our consolidated application results unfold new opportunities for MIPG-fluorescent sensors for environmentally and medicinally important analytes detection.

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Thermo-responsive imprinted hydrogel with switchable sialic acid recognition for selective cancer cell isolation from blood

Cited by 46 publications

References 45 publications

Molecular Imprinting Strategies for Tissue Engineering Applications: A Review

Molecular Imprinting Strategies for Tissue Engineering Applications: A Review

Selective recognition of tumor cells by molecularly imprinted polymers

Typical Fluorescent Sensors Exploiting Molecularly Imprinted Hydrogels for Environmentally and Medicinally Important Analytes Detection

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