Thermo‐Responsive Polymer Brushes as Intelligent Biointerfaces: Preparation via ATRP and Characterization

Nagase, Kenichi; Watanabe, Minami; Kikuchi, Akihiko; Yamato, Masayuki; Okano, Teruo

doi:10.1002/mabi.201000312

Cited by 90 publications

(43 citation statements)

References 41 publications

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“…Especially, the ATRP technique has been developed rapidly due to the advantages of being simple, inexpensive, and more general for controlled radical polymerization when it was firstly proposed by Wang and Matyjaszewsi [17]. There are a greater number of researchers using ATRP to functionalize SNPs [18-22]. …”

Section: Introductionmentioning

confidence: 99%

Silica nanoparticles functionalized via click chemistry and ATRP for enrichment of Pb(II) ion

Zhou

et al. 2012

Nanoscale Res Lett

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Silica nanoparticles have been functionalized by click chemistry and atom transfer radical polymerization (ATRP) simultaneously. First, the silanized silica nanoparticles were modified with bromine end group, and then the azide group was grafted onto the surface via covalent coupling. 3-Bromopropyl propiolate was synthesized, and then the synthesized materials were used to react with azide-modified silica nanoparticles via copper-mediated click chemistry and bromine surface-initiated ATRP. Transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis were performed to characterize the functionalized silica nanoparticles. We investigated the enrichment efficiency of bare silica and poly(ethylene glycol) methacrylate (PEGMA)-functionalized silica nanoparticles in Pb(II) aqueous solution. The results demonstrated that PEGMA-functionalized silica nanoparticles can enrich Pb(II) more quickly than pristine silica nanoparticles within 1 h.

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Section: Introductionmentioning

confidence: 99%

Silica nanoparticles functionalized via click chemistry and ATRP for enrichment of Pb(II) ion

Zhou

et al. 2012

Nanoscale Res Lett

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“…This PIPAAm brush has also been used as cell culture substrate. PIPAAm brushes with adequate chain lengths on glass or polystyrene substrates have exhibited effective thermally controlled on–off adhesion/detachment behavior of endothelial cells 28, 29…”

Section: Introductionmentioning

confidence: 99%

Thermally Modulated Retention of Lymphoctytes on Polymer‐Brush‐Grafted Glass Beads

Nagase

Mukae

Kikuchi

et al. 2011

Macromolecular Bioscience

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Thermoresponsive PIPAAm-brush-grafted glass beads are prepared through siATRP and their physicochemical properties are characterized by micro-nitrogen analysis, XPS, and contact angle measurements. The amount of grafted PIPAAm on glass bead surfaces can be controlled by varying the ATRP reaction time, leading to a modulation of the temperature-dependent wettability of the prepared surfaces. To evaluate a possible use of the beads as cell separation matrices, loading with rat lymphocytes from mesenteric lymph nodes is studied. The results show that the interaction between PIPAAm brushes and lymphocytes can be controlled by modulating PIPAAm brush length and temperature. The PIPAAm-brush-grafted beads might therefore be useful as effective cell separation matrices.

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“…Other groups reported that plasma activation and plasma polymerization were also available for the surface modification with PIPPAm [41]. Recently, to precisely control chain length and density of grafted PIPAAm on the surfaces, atom transfer radical polymerization (ATRP) or reversible addition-fragmentation chain transfer (RAFT) polymerization are also used, which contributes to enhanced handling of cell attachment or detachment [42][43][44][45][46]. Furthermore, we have also developed the method to fabricate a cell sheet using the thermoresponsive surfaces that are prepared via a spin-coating method with a block copolymer consisting of PIPAAm and poly(butyl methacrylate) (PBMA) on polystyrene surfaces [47].…”

Section: Cell Sheet Engineering Based On Temperatureresponsive Culturmentioning

confidence: 99%

Regenerative medicine of cornea by cell sheet engineering using temperature-responsive culture surfaces

et al. 2013

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Recently, regenerative medicine has been focused on as next-generation definitive therapies for several diseases or injuries for which there are currently no effective treatments. These therapies have been rapidly developed through the effective fusion between different fields such as stem cell biology and biomaterials. So far, we have proposed "cell sheet engineering" through our core technology that simply applies alterations of the temperature which allows regulation of the attachment or detachment of living cells on the culture surfaces grafted with the temperature-responsive polymer "poly(N-isoproplyacrylamide)". This technology enables us to construct bioengineered sheet-like tissues, termed "cell sheets", without the need of using biodegradable scaffolds and protease treatments that are traditionally used. Therefore, our carrier-free cell sheets not only are independent of the issues observed in conventional methods, but also showed further advantages in the reconstruction of the corneal epithelium or endothelium (e.g. improvement of optical transparency and cell-cell interactions to host stroma in reconstructed tissues). Moreover, our approach does not have issues such as immune rejection or limited number of donors, due to the use of cell sheets derived from autologous limbal (in patients with unilateral disease) or oral mucosal epithelial cells (in patients with bilateral disorders). Indeed, we have successfully performed the clinical application for the reconstruction of ocular surfaces through the transplantation of our carrier-free corneal epithelial cell sheets, as evidenced by improved visual acuity as well as long-term maintenance of postoperative health conditions on ocular surfaces in all patients. We have also proposed a novel approach for the reconstruction of the corneal endothelium using corneal endothelial cell sheets by demonstrating its successful application. Thus, our cell sheet engineering technique provides a breakthrough in the field of regenerative medicine applied to the cornea. cell sheet, temperature-responsive, carrier-free Citation:Umemoto T, Yamato M, Nishida K, et al. Regenerative medicine of cornea by cell sheet engineering using temperature-responsive culture surfaces. Chin Sci Bull, 2013, 58: 43494356,

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Thermo‐Responsive Polymer Brushes as Intelligent Biointerfaces: Preparation via ATRP and Characterization

Cited by 90 publications

References 41 publications

Silica nanoparticles functionalized via click chemistry and ATRP for enrichment of Pb(II) ion

Silica nanoparticles functionalized via click chemistry and ATRP for enrichment of Pb(II) ion

Thermally Modulated Retention of Lymphoctytes on Polymer‐Brush‐Grafted Glass Beads

Regenerative medicine of cornea by cell sheet engineering using temperature-responsive culture surfaces

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