Thermo‐responsive polymeric surfaces; control of attachment and detachment of cultured cells

Yamada, Noriko; Okano, Teruo; Sakai, Hideaki; Karikusa, Fumiko; Sawasaki, Yoshio; Sakurai, Yasuhisa

doi:10.1002/marc.1990.030111109

Cited by 943 publications

(394 citation statements)

References 9 publications

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“…Using electron irradiation, uniformly spread IPAAm monomers were polymerized and covalently immobilized onto the surface of the tissue culture polystyrene dish (TCPS) [21][22][23]. Although electron irradiation requires expensive equipment, this method facilitates large-scale production of thermoresponsive TCPS.…”

Section: Preparation and Characterization Of Pipaam-grafted Surfacesmentioning

confidence: 99%

“…Since 1990, our group has been developing the thermoresponsive cell culture dish grafted with PIPAAm on the surface, allowing cultured cells to be recovered by simply lowering the temperature below LCST (figure 2(a)) [21][22][23]. When the temperature is lowered, morphological changes to cells and the detachment of cells are suppressed by an ATP synthesis inhibitor, sodium azide, and a tyrosine kinase inhibitor, genistein [23,34].…”

Section: Thermoresponsive Cell Culture Dishmentioning

confidence: 99%

“…The 'deadhesion' process is inherent on the surface of the thermoresponsive cell culture dish. The cells recovered from the thermoresponsive cell culture dish retain their cellular structure and functions, while trypsinization causes damage to the cell membrane and ECM [21][22][23]. Thus, the cell culture method using the thermoresponsive cell culture dish is considered a powerful tool for investigating the molecular machinery involved in cell-surface detachment.…”

Section: Thermoresponsive Cell Culture Dishmentioning

confidence: 99%

“…This alteration of the PIPAAm-modified intelligent surface can be used to modulate its interactions with solutes in the chromatographic separation [15][16][17][18][19][20] and with cultured cells [21][22][23]. PIPAAm-grafted chromatographic matrices allow us to regulate the retention of steroids [15,16], polypeptides and proteins [17,18], and nucleotides [19,20] by changing the temperature under the aqueous condition.…”

Section: Introductionmentioning

confidence: 99%

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Fabrication of a thermoresponsive cell culture dish: a key technology for cell sheet tissue engineering

Kobayashi¹,

Okano²

2010

Science and Technology of Advanced Materials

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This article reviews the properties and characterization of an intelligent thermoresponsive surface, which is a key technology for cell sheet-based tissue engineering. Intelligent thermoresponsive surfaces grafted with poly(N-isopropylacrylamide) exhibit hydrophilic/hydrophobic alteration in response to temperature change. Cultured cells are harvested on thermoresponsive cell culture dishes by decreasing the temperature without the use of digestive enzymes or chelating agents. Our group has developed cell sheet-based tissue engineering for therapeutic uses with single layer or multilayered cell sheets, which were recovered from the thermoresponsive cell culture dish. Using surface derivation techniques, we developed a new generation of thermoresponsive cell culture dishes to improve culture conditions. We also designed a new methodology for constructing well-defined organs using microfabrication techniques.

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Section: Preparation and Characterization Of Pipaam-grafted Surfacesmentioning

confidence: 99%

Section: Thermoresponsive Cell Culture Dishmentioning

confidence: 99%

Section: Thermoresponsive Cell Culture Dishmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Fabrication of a thermoresponsive cell culture dish: a key technology for cell sheet tissue engineering

Kobayashi¹,

Okano²

2010

Science and Technology of Advanced Materials

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“…33 On the other hand, there is a major technical barrier to the use of micropatterned cells for future transplantation, because of difficulties in dissociating cells from substrates, such as glass, silicon, gold, metal oxides and polymeric materials, without a loss of the constructed structures. Creating cell micropatterns on biocompatible or biodegradable biomaterials 34,35 or poly(Nisopropylacrylamide), [36][37][38] a smart material that allows dissociation of a contiguous cell sheet accompanying extracellular matrices, overcomes this issue. In addition to these micropatterned tissue mimics, cultured non-differentiated pluripotent stem cells are an important source for regenerative medicine.…”

Section: ·3 Tissue Engineeringmentioning

confidence: 99%

Recent Advances in Cell Micropatterning Techniques for Bioanalytical and Biomedical Sciences

et al. 2008

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Cell micropatterning is a method for controlling the placement of living cells on a substrate surface. [1][2][3][4][5][6][7][8] It is important for a wide range of applications, such as tissue engineering, cellbased drug screening, and fundamental cell biology studies. Most cell micropatterning methods fall into three categories based on strategy: (1) seeding cells on a chemically patterned surface of different cell adhesiveness, (2) seeding cells on a topographically patterned surface, or (3) directed delivery of cells onto discrete regions of a substrate. Although the latter two categories are also important, this review mainly focuses on the first category. This is because it is the most common strategy and it provides tools to study fundamental aspects of cell adhesion at the single protein/receptor molecule level. 9There are excellent reviews that also cover the latter two categories. 3,8,10 We first introduce some of the important applications of cell micropatterning in general. We then describe methods for micropatterning the cell adhesiveness, referring to materials that promote or inhibit cell adhesion. We then move on to much more sophisticated substrates, so-called "dynamic substrates", whose cell adhesiveness can be changed by an external stimulus, such as heat, voltage and light. As an example of dynamic substrates, we describe a caged culture substrate developed by our group. This type of functional substrate opens up new possibilities in bioanalytical and biomedical sciences. Cell micropatterning is an important technique for a wide range of applications, such as tissue engineering, cell-based drug screening, and fundamental cell biology studies. This paper overviews cell patterning techniques based on chemically modified substrates with different degrees of cell adhesiveness. In particular, the focus is on dynamic substrates that change their cell adhesiveness in response to external stimuli, such as heat, voltage, and light. Such substrates allow researchers to achieve an in situ alteration of patterns of cell adhesiveness, which is useful for coculturing multiple cell types and analyzing dynamic cellular activities. As an example of dynamic substrates, we introduce a dynamic substrate based on a caged compound, where we accomplished a light-driven alteration of cell adhesiveness and the analysis of a single cell's motility.

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Bioengineered Human Corneal Endothelium for Transplantation

Lai¹

2006

Arch Ophthalmol

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Thermo‐responsive polymeric surfaces; control of attachment and detachment of cultured cells

Cited by 943 publications

References 9 publications

Fabrication of a thermoresponsive cell culture dish: a key technology for cell sheet tissue engineering

Fabrication of a thermoresponsive cell culture dish: a key technology for cell sheet tissue engineering

Recent Advances in Cell Micropatterning Techniques for Bioanalytical and Biomedical Sciences

Bioengineered Human Corneal Endothelium for Transplantation

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