Thermoactivation of Pseudomonas aeruginosa Pyocins

Balko, O.I.; Balko, O.B.; Avdeeva, L.V.

doi:10.15407/microbiolj81.05.085

Cited by 8 publications

(24 citation statements)

References 10 publications

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“…The objects of inves tigation were P. aeruginosa strains -UCM В-9, UCM В-330, UCM В-332, UCM В-333, UCM В-335, maintained in Ukrainian collection of microorganisms (UCM, Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine). These strains of microorganisms are producers of highly active bacteriocins, corresponding data were published previously [18].…”

Section: Methodsmentioning

confidence: 75%

“…The obtained supernatants were aseptically removed and stored in closed containers at 4-6 °C. Chloroform was used as a preserving agent [19].…”

Section: Methodsmentioning

confidence: 99%

“…Antimicrobial activity at all separation stages was tested by "two-layer agar" method [19]. Quantitative indices of lysate activities were determined by double serial dilutions method, substance activity was estimated according to the maximal dilution able to cause formation of lysis zone.…”

Section: Methodsmentioning

confidence: 99%

“…The obtained results were counted for 1 ml of studied lysate and expressed in activity units -AU/mL or for convenience in ×10 3 AU/mL. P. aeru ginosa UCM В-3 and UCM В-10 were used as indicator cultures [18].…”

Section: Methodsmentioning

confidence: 99%

“…They don't influence own producer strains, lose activity after trypsin treatment (1 mg/mL) and their absorption peaks amount to 205-210 nm, which is characteristic for peptide bond of proteins. We assumed that noted bacteriocins can be associated with S-type pyocins, produced by the same strains [15]. The relationship between different types of bacteriocins has been suggested by some authors [16], but not shown for pyocins.…”

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confidence: 94%

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Interaction between S-Type Pyocins and Microcin-II-Like Bacteriocins in Pseudomonas aeruginosa

Balko¹

2021

Mikrobiol. Z.

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According to our previous results, S-type bacteriocins of Pseudomonas aeruginosa are characterized by high activity against phytopathogenic Pseudomonas syringae strains. In addition to these pyocins producing strains are able to synthesize microcin-II-like bacteriocins. Presence of interaction between these two killer factors can determine methods of their use and activity increase of bacteriocins with antiphytopathogenic properties. The aim of the work was to test possibility of interaction between S-type pyocins and microcin-II-like bacteriocins of P. aeruginosa. Methods. The objects of the study were pyocins produced by 6 P. aeruginosa strains. Killer factors in composition of induced lysates were concentrated by 70% ammonium sulphate precipitation, dialyzed through dialysis membrane with molecular weight cut-off (MWCO) 3.5 kDa. Then ion-exchange chromatography with DEAE-cellulose, gel filtration with Sephadex G-75 and ultracentrifugation at 215.000 g for 1 and 4 hours were used for their separation. Protein concentration and antimicrobial activity were determined in obtained fractions. Visualization of proteins in active fraction composition was conducted by electrophoresis according to the Laemmli method. Results. Under ion-exchange chromatography with DEAE-cellulose application elution of bacteriocins available in lysate composition occurs simultaneously. The highest indices of activity and protein concentration were in the 4th fraction, containing two protein bands with molecular weight near 58 and 9 kDa, which are typical for S5 pyocin and microcin-II-like bacteriocins of P. aeruginosa. Further gel filtration of sampled fractions through Sephadex G-75 allowed to separate noted killer factors and obtaine purified fraction containing microcin-II-like pyocins only. Application of ultracentrifugation during 1 hour didn’t precipitate studied bacteriocins, whereas during 4 hours – lead to their separation. At the same time a twofold increase of activity indices for S-type pyocins in precipitates and for microcin-IIlike killer factors – in supernatants were observed. However achieved concentration was characterized by short-term effect, since in 14 days activity of supernatants decreased by 4–16 times, and for precipitates – by 80–640 times. Then revealed tendency for activity decrease continued. Conclusions. S-type pyocins and microcin-II-like bacteriocins of P. aeruginosa interact with each other, that ensures their stabilization and protects again destruction. Application of methods that cause separation of these killer factors is inexpedient, since it results into considerable decrease of bacteriocin activity indices.

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Section: Methodsmentioning

confidence: 75%

“…The obtained supernatants were aseptically removed and stored in closed containers at 4-6 °C. Chloroform was used as a preserving agent [19].…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 94%

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Interaction between S-Type Pyocins and Microcin-II-Like Bacteriocins in Pseudomonas aeruginosa

Balko¹

2021

Mikrobiol. Z.

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A superlinear lower bound on the number of 5-holes

Aichholzer¹,

Balko²,

Hackl³

et al. 2020

Journal of Combinatorial Theory, Series A

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Let P be a finite set of points in the plane in general position, that is, no three points of P are on a common line. We say that a set H of five points from P is a 5-hole in P if H is the vertex set of a convex 5-gon containing no other points of P . For a positive integer n, let h 5 (n) be the minimum number of 5-holes among all sets of n points in the plane in general position.Despite many efforts in the last 30 years, the best known asymptotic lower and upper bounds for h 5 (n) have been of order Ω(n) and O(n 2 ), respectively. We show that h 5 (n) = Ω(n log 4/5 n), obtaining the first superlinear lower bound on h 5 (n).The following structural result, which might be of independent interest, is a crucial step in the proof of this lower bound. If a finite set P of points in the plane in general position is partitioned by a line into two subsets, each of size at least 5 and not in convex position, then intersects the convex hull of some 5-hole in P . The proof of this result is computer-assisted.

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Low Molecular Weight Pseudomonas aeruginosa Bacteriocins

Balko¹

2019

Mikrobiol. Z.

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Thermoactivation of Pseudomonas aeruginosa Pyocins

Cited by 8 publications

References 10 publications

Interaction between S-Type Pyocins and Microcin-II-Like Bacteriocins in Pseudomonas aeruginosa

Interaction between S-Type Pyocins and Microcin-II-Like Bacteriocins in Pseudomonas aeruginosa

A superlinear lower bound on the number of 5-holes

Low Molecular Weight Pseudomonas aeruginosa Bacteriocins

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