Thermodynamic approach to estimating reactions and stoichiometric coefficients of anaerobic glucose and hydrogen utilization

Wresta, Arini; Widyarani, Rani; Boopathy, Ramaraj; Setiadi, Tjandra

doi:10.1002/eng2.12347

Cited by 6 publications

(3 citation statements)

References 32 publications

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“…+ 1.5𝐻 ! 𝑂 + 0.2𝐻 # for biomass production (21 mole-/molNbiomass) (Wresta et al, 2021) (2) 𝑁 ! + 3𝐻 !…”

Section: Design Of Microbial Electrochemical Systemmentioning

confidence: 99%

Comparison of enrichment methods for efficient nitrogen fixation on a biocathode

Rous,

Santa-Catalina,

Desmond-Le Quémener

et al. 2024

Peer Community Journal

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The production of nitrogen fertilizers in modern agriculture is mostly based on the Haber-Bosch process, representing nearly 2% of the total energy consumed in the world. Lowenergy bioelectrochemical fixation of N 2 to microbial biomass was previously observed but the mechanisms of microbial interactions in N 2 -fixing electroactive biofilms are still poorly understood. The present study aims to develop a new method of enrichment of autotrophic and diazotrophic bacteria from soil samples with a better electron source availability than using H 2 alone. The enrichment method was based on a multi-stage procedure. The first enrichment step was specifically designed for the selection of N 2 -fixing bacteria from soil samples with organic C as electron and carbon source. Then, a polarized cathode was used for the enrichment of autotrophic bacteria using H 2 (hydrogenotrophic) or the cathode as electron source. This enrichment was compared with an enrichment culture of pure diazotrophic hydrogenotrophic bacteria without the use of a microbial electrochemical system. Interestingly, both methods showed comparable results for N 2 fixation rates at day 340 of the enrichment with an estimated average of approximately 0.2 mgN fixed /L.d. Current densities up to -15 A/m² were observed in the polarized cathode enrichments and a significant increase of the microbial biomass on the cathode was shown between 132 and 214 days of enrichment.These results confirmed an enrichment in autotrophic and diazotrophic bacteria on the polarized cathode. It was hypothesied that autotrophic bacteria were able to use either the H 2 produced at the cathode or directly the cathode through direct electron transfer (DET) as more biomass was produced than with H 2 alone. Finally, the analysis of the enriched communities suggested that Desulforamulus ruminis mediated microbial interactions between autotrophic anaerobic and heterotrophic aerobic bacteria in polarized cathode enrichment. These interactions could play a key role in the development of biomass in these systems and on N 2 fixation. Based on these findings, a conceptual model on the functioning of mixed cultures N 2 -fixing electroactive biofilms was proposed.

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“…+ 1.5𝐻 ! 𝑂 + 0.2𝐻 # for biomass production (21 mole-/molNbiomass) (Wresta et al, 2021) (2) 𝑁 ! + 3𝐻 !…”

Section: Design Of Microbial Electrochemical Systemmentioning

confidence: 99%

Comparison of enrichment methods for efficient nitrogen fixation on a biocathode

Rous,

Santa-Catalina,

Desmond-Le Quémener

et al. 2024

Peer Community Journal

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show abstract

“…Eq. 1 2.1 ‫ܪ‬ ଶ ‫ܱܥ‬ ଶ ‫ܪܰ2.0‬ ସ ା ՜ ‫ܪܥ‬ ଵ.଼ ܱ .ହ ܰ .ଶ ‫ܪ5.1‬ ଶ ܱ ‫ܪ2.0‬ ା for biomass production (21 mol e-/mol Nbiomass ) (Wresta et al, 2021) Eq. 2 ܰ ଶ ‫ܪ3‬ ଶ ՜ ‫ܪܰ2‬ ଷ for nitrogen fixation (3 mol e-/mol Nfixé ) (L. Eq.…”

Section: Culture Mediamentioning

confidence: 99%

“…2 ܰ ଶ ‫ܪ3‬ ଶ ՜ ‫ܪܰ2‬ ଷ for nitrogen fixation (3 mol e-/mol Nfixé ) (L. Eq. 3 ‫ܱܥ2‬ ଶ ‫ܪ4‬ ଶ ՜ ‫ܪܥ‬ ଷ ‫ܱܱܥ‬ ି ‫ܪ‬ ା ‫ܪ2‬ ଶ ܱ for acetate production (8 mol e-/mol CH3COO-) (Wresta et al, 2021) Eq. 4…”

Section: Culture Mediamentioning

confidence: 99%

Comparison of enrichment methods for efficient nitrogen fixation on a biocathode

Rous

Santa-Catalina

Quéméner

et al. 2023

Preprint

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The production of nitrogen fertilizers in modern agriculture is mostly based on the Haber-Bosch process, representing nearly 2% of the total energy consumed in the world. Low-energy bioelectrochemical fixation of N2to microbial biomass was previously observed on a biocathode but the microbial interactions in N2-fixing electroactive biofilms are still poorly understood. The present study aims to develop a method of enrichment of autotrophic and diazotrophic bacteria from soil samples for further characterization. The enrichment method was based on a first classical step of selection of N2-fixing bacteria from soil samples. Then, a polarized cathode was used for the enrichment of autotrophic bacteria using H2(hydrogenotrophic) or the cathode as energy sources. This enrichment was compared with an enrichment of diazotrophic hydrogenotrophic bacteria without the use of the microbial electrochemical system. Both methods showed comparable results for N2fixation rates at day 340 of the enrichment, with an estimated average of approximately 0.2 mgNfixed/L.d. Current densities up to -15 A/m² were observed in the polarized cathode enrichments and a significant increase of the microbial biomass on the cathode was shown between 132 and 214 days of enrichment. These results confirm the enrichment of autotrophic, electrotrophic and diazotrophic bacteria in the polarized cathode enrichments. Finally, the analysis of the enriched communities suggest thatDesulforamulus ruminismediated microbial interactions between autotrophic anaerobic and heterotrophic aerobic bacteria in polarized cathode enrichment. These interactions could play a key role in the development of biomass in these systems and on N2fixation. Based on these findings, a conceptual model on the functioning of mixed cultures N2-fixing electroactive biofilms was proposed.

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Central and peripheral regulations mediated by short-chain fatty acids on energy homeostasis

Luo¹,

Lednovich²,

Xu³

et al. 2022

Translational Research

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Thermodynamic approach to estimating reactions and stoichiometric coefficients of anaerobic glucose and hydrogen utilization

Cited by 6 publications

References 32 publications

Comparison of enrichment methods for efficient nitrogen fixation on a biocathode

Comparison of enrichment methods for efficient nitrogen fixation on a biocathode

Comparison of enrichment methods for efficient nitrogen fixation on a biocathode

Central and peripheral regulations mediated by short-chain fatty acids on energy homeostasis

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