Thermodynamic characteristics of mixed monolayers of amphotericin B and cholesterol

Recent progress in the study of the interactions of amphotericin B with cholesterol and ergosterol in lipid environments

“…Permeable ion-channels are also formed in the presence of cholesterol, as was investigated by Yilma et al ( 2007 ) for a cholesterol monolayer. Similar to Sykora et al ( 2004 ) and, they found that AmB and cholesterol form a complex with of stoichiometry 2:1, but Yilma et al also showed that AmB in the presence of cholesterol assembles in three, four, etc., subunit aggregates which form ion channels. According to the above-mentioned studies, it is most probable that the AmB–cholesterol interaction is the main factor responsible for toxicity to mammalian cells whereas the interaction between AmB derivatives and lipids is less important for toxicity.…”

N-(1-Piperidinepropionyl)amphotericin B methyl ester (PAME)— a new derivative of the antifungal antibiotic amphotericin B: Searching for the mechanism of its reduced toxicity

“…Although the monolayer experiments for mixtures of AmB and sterols/lipids have already been published [3,13,[30][31][32][33][34]36] in none of these studies was dimethylformamide (DMF) used to dissolve all the molecules investigated. The spreading solvent affects the shape of AmB isotherms as well as the stoichiometry of the antibiotic/sterol complexes.…”

Thermodynamic characterization of the prevailing molecular interactions in mixed floating monolayers of phospholipids and usnic acid

“…Therefore, the analysis of the thermodynamic behavior of mixed monolayers is a valuable method for elucidating a number of issues about liposome stability [6,7]. Important questions about the physicochemical aspects of the floating monolayer, such as dominating molecular interactions, the nature of the bonding forces, and the organization of the phospholipid complex formed, can be Scheme 1. addressed by assessing the corresponding thermodynamic parameters [8,9].…”