Thermodynamic Modeling of Solvent-Assisted Lipid Bilayer Formation Process

Xu, Hongmei; Tae, Hyunhyuk; Cho, Nam‐Joon; Huang, Changjin; Hsia, K. Jimmy

doi:10.3390/mi13010134

Cited by 5 publications

(4 citation statements)

References 49 publications

(69 reference statements)

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“…Again, phenomenological modeling supported the lipid concentration dependency of bilayer formation (Figure G) . The linear relationship between surface coverage of SLBs and lipid concentration at a low range was also predicted by thermodynamic modeling and QCM-D experimental measurements, which provides a practical guideline to procure quantitative conditions for the SALB strategy …”

Section: Factors For Solvent-assisted Lipid Assemblysupporting

confidence: 61%

Section: Factors For Solvent-assisted Lipid Assemblymentioning

confidence: 99%

“…The SALB strategy solves the challenge of requiring a processing temperature higher than the lipids transition temperature in the vesicle fusion method . To elucidate the temperature effect, thermodynamic modeling was executed for the SALB procedure . On the basis of experimental data with this model, a phase diagram with DPPC SLB coverage was obtained as a function of temperature and lipid concentration (Figure I).…”

Section: Factors For Solvent-assisted Lipid Assemblymentioning

confidence: 99%

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Artificial Cell Membrane Platforms by Solvent-Assisted Lipid Bilayer (SALB) Formation

2022

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Conspectus Supported lipid bilayers (SLBs) are artificial cell membrane platforms that recapitulate key aspects of lipid membrane architecture on material surfaces. Their compatibility with a variety of surface-sensitive biophysical techniques highlights the importance of designing and constructing SLBs on target materials. Moreover, the functionalization of SLBs by modulating the lipid compositions and/or incorporating biomolecules provides versatile opportunities to improve understanding of membrane properties and molecular interaction as well as the applications to analytic platforms and diagnostic biosensing. To date, the most favored approach to form SLBs is vesicle fusion, which involves vesicle adsorption and simultaneous rupture on a solid surface. However, this technique is highly dependent on the vesicle preparation, lipid compositions, and types of surface materials. Within this context, there have been tremendous efforts to advance fabrication technology surpassing vesicle fusion, which has led to the development of next-generation SLB platforms and opened the door to a wide range of new applications such as diagnostic biosensors, biocompatible coatings, and bioanalytical tools. In this Account, we summarize recent progress in the innovative SLB fabrication technique termed the solvent-assisted lipid bilayer (SALB) method, which our group has successfully developed to transcend the conventional vesicle fusion method. We particularly focus on the material aspects of the biomimetic SLB platform, including solid substrates and lipid compositions, which can be extended by SALB method. Along with the principles of lipid molecular self-assembly, we first introduce the development of SALB method and compelling advantages of this strategy that integrates simple sample preparation, affinity with a wide range of material supports, and various lipid compositions by comparing with vesicle fusion method. We systematically describe how this approach can be effectively employed to extensive solid substrates and broad lipid compositions via combination of theoretical simulation modeling and experimental analysis by cutting-edge surface-sensitive characterization techniques that have been utilized in our group for the biointerfacial analysis, involving fluorescence microscopy and quartz crystal microbalance with dissipation monitoring (QCM-D). We then critically discuss important exploratory parameters for solvent-assisted lipid self-assembly underpinning this strategy including flow rate, lipid concentrations, types of organic solvent, and temperature to improve fundamental understanding and optimize quantitative conditions. Finally, we present recent application examples encompassing biocompatible antifouling coating, biomolecular interaction monitoring, and extracellular matrix remodeling. With the ongoing development and application of the SALB method, there is a future opportunity to enrich our fundamental understanding of biointerfacial science and lead to new technological breakthroughs and application possibil...

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Section: Factors For Solvent-assisted Lipid Assemblysupporting

confidence: 61%

Section: Factors For Solvent-assisted Lipid Assemblymentioning

confidence: 99%

Section: Factors For Solvent-assisted Lipid Assemblymentioning

confidence: 99%

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Artificial Cell Membrane Platforms by Solvent-Assisted Lipid Bilayer (SALB) Formation

2022

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“…When the aqueous buffer, in this case, bidistilled water, is suddenly added to the lipidic mixture, the lipids reorganize themselves and spontaneously self-assemble into various structures. Among these structures, lipid bilayers on the available substrates (in the present case, the hydrophilic surface offered by zinc oxide NPs) are the most energetically favorable [43]. To achieve this goal, the amount of water added to the lipidic mixture was finely tuned to be equal to at least 10 times the volume of the organic solvent.…”

Section: Solvent Exchange Technique For Iron-doped Zinc Oxide Nanopar...mentioning

confidence: 99%

Lipidic Formulations Inspired by COVID Vaccines as Smart Coatings to Enhance Nanoparticle-Based Cancer Therapy

et al. 2023

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Recent advances in nanomedicine have led to the introduction and subsequent establishment of nanoparticles in cancer treatment and diagnosis. Nonetheless, their application is still hindered by a series of challenges related to their biocompatibility and biodistribution. In this paper, we take inspiration from the recently produced and widely spread COVID vaccines, based on the combinational use of ionizable solid lipid nanoparticles, cholesterol, PEGylated lipids, and neutral lipids able to incorporate mRNA fragments. Here, we focus on the implementation of a lipidic formulation meant to be used as a smart coating of solid-state nanoparticles. The composition of this formulation is finely tuned to ensure efficient and stable shielding of the cargo. The resulting shell is a highly customized tool that enables the possibility of further functionalizations with targeting agents, peptides, antibodies, and fluorescent moieties for future in vitro and in vivo tests and validations. Finally, as a proof of concept, zinc oxide nanoparticles doped with iron and successively coated with this lipidic formulation are tested in a pancreatic cancer cell line, BxPC-3. The results show an astonishing increase in cell viability with respect to the same uncoated nanoparticles. The preliminary results presented here pave the way towards many different therapeutic approaches based on the massive presence of highly biostable and well-tolerated nanoparticles in tumor tissues, such as sonodynamic therapy, photodynamic therapy, hyperthermia, and diagnosis by means of magnetic resonance imaging.

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Lipid Microfluidic Biomimetic Models for Drug Screening: A Comprehensive Review

Fernandes,

Cardoso,

Lanceros‐Méndez

et al. 2024

Adv Funct Materials

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Developing new drugs is a complex, time‐consuming, and expensive process, essential to identify potential pharmacokinetic issues in the early stages to avoid investment in nonpromising candidates. Nearly half of all drug targets and key drug‐metabolizing enzymes are found in intracellular environments, compartmentalized by semipermeable barriers, the cell membranes. Inspired by their lipidic bilayer structure, lipid‐based biomimetic platforms are being developed to understand the biochemical and biophysical processes at the cellular membrane level. Considering the pharmaceutical industry's demands for efficient in vitro high throughput screening tools, microfluidic technology emerges as a promising solution to address challenges in lipid biomimetic models for screening applications. This involves the transformation of commonly used lipid‐based biomimetic models, like supported lipid bilayers and lipid vesicles, to miniaturized approachs and their evolution into a new generation of bilayer models. These include pore‐suspended and free‐standing lipid bilayers, black lipid membranes, and droplet interface bilayers. This review provides an overview of both generations of lipid biomimetic models used in drug‐membrane screening applications. Moreover, it delves into the intricacies of their production through microfluidic approaches and examines their screening applications in drug‐membrane interaction. The review concludes with a critical analysis of potential future directions in this rapidly evolving field.

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Thermodynamic Modeling of Solvent-Assisted Lipid Bilayer Formation Process

Cited by 5 publications

References 49 publications

Artificial Cell Membrane Platforms by Solvent-Assisted Lipid Bilayer (SALB) Formation

Artificial Cell Membrane Platforms by Solvent-Assisted Lipid Bilayer (SALB) Formation

Lipidic Formulations Inspired by COVID Vaccines as Smart Coatings to Enhance Nanoparticle-Based Cancer Therapy

Lipid Microfluidic Biomimetic Models for Drug Screening: A Comprehensive Review

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