We provide a minimal strategy for the quantitative analysis of a large class of non-equilibrium systems in a steady state using the short-time Thermodynamic Uncertainty Relation (TUR).
From short-time trajectory data obtained from experiments, we demonstrate how we can simultaneously infer quantitatively, both the
thermodynamic force field acting on the system, as well as the exact rate of entropy production. We benchmark this scheme first for an experimental study of a colloidal particle system where exact analytical results are known, before applying it to the case of a colloidal particle in a hydrodynamical flow field, where neither analytical nor numerical results are available. Our scheme hence provides a means, potentially exact for a large class of systems, to get a quantitative estimate of the entropy produced in maintaining a non-equilibrium system in a steady state, directly from experimental data.