Thermodynamics and In-Plane Viscoelasticity of Anionic Phospholipid Membranes Modulated by an Ionic Liquid

Hitaishi, Prashant; Seth, Ajit; Mitra, Saheli; Ghosh, Sajal K.

doi:10.1007/s11095-022-03348-1

Cited by 9 publications

(15 citation statements)

References 75 publications

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“…The surface pressure–area isotherm generated by a Langmuir trough is useful for studying interactions among amphiphilic molecules that form a single molecular layer at the air–water interface. ,,− Researchers have adopted this approach to understand the thermodynamics of the interaction of ILs with lipids. ,, It has been reported that the polar head group and hydrophobicity of lipids and the nature of anions and cations of ILs along with the pH of the environment play significant roles in this interaction. , Apart from electrostatics and van der Waals interactions, the thermodynamic state of a lipid in the membrane is important here . Further, as mentioned in the Introduction section, the interaction between a lipid and an IL may highly be influenced by the presence of other membranous component, such as cholesterol. , In the present study, the respective isotherms are generated by plotting the surface pressure against the area per molecule, which is calculated by considering the number of molecules of lipid (single component), lipid and IL (two components), and lipid, cholesterol, and IL (three components).…”

Section: Resultsmentioning

confidence: 99%

“…The incorporation of IL in the compact film randomizes the molecular conformation, which might make the film less rigid and less viscous. This observation is consistent with our previous study, which suggested that the increase in the system’s entropy is responsible for the decrease in the rigidity of the membrane. This effect is weak in the LE phase as the lipid molecules are inherently in their high entropy state due to the highly accessible area.…”

Section: Resultsmentioning

confidence: 99%

“…The amplitude of the oscillation was taken to be 1% with respect to the total lipid film area. The relevant equations are − italica ( t ) = A ( 1 + a 0 sin false( ω t + ϕ normala false) ) π ( t ) = P + π 0 0.25em sin ( ω t + ϕ π ) where A , a 0 , and a ( t ) are the initial surface area, the amplitude of the applied strain (1%), and the time-dependent change in the area, respectively. ω is the angular frequency of oscillation, π 0 is the stress amplitude, and P is the initial surface pressure.…”

Section: Methodsmentioning

confidence: 99%

“…Because of the viscoelastic nature of the film, there is a phase lag between stress and strain, which is given by ϕ = ϕ π – ϕ a . The two viscoelastic parameters were calculated to estimate the elastic and viscous nature of the monolayer represented as the storage modulus ( G ′) and the loss modulus ( G ″), respectively, from the complex modulus expressed as ,,, G * = π 0 a 0 0.25em cos nobreak0em.25em⁡ ϕ + normali π 0 a 0 0.25em sin nobreak0em.25em⁡ ϕ = G ′ + normali G ″ …”

Section: Methodsmentioning

confidence: 99%

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Cholesterol-Controlled Interaction of Ionic Liquids with Model Cellular Membranes

et al. 2023

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While ionic liquids (ILs) are considered as prospective ingredients of new antimicrobial agents, it is important to understand the adverse effects of these molecules on human cells. Since cholesterol is the essential component of a human cell membrane, in the present study, the effect of an imidazolium-based IL has been investigated on the model membrane in the presence of cholesterol. The area per sphingomyelin lipid is found to reduce in the presence of the IL, which is quantified by the area−surface pressure isotherm of the lipid monolayer formed at the air−water interface. The effect is considerably diminished in the cholesterolcontaining monolayer. Further, the IL is observed to decrease the rigidity of the cholesterol-free monolayer. Interestingly, the presence of cholesterol does not allow any change in this property of the layer at lower surface pressure. However, at a higher surface pressure, the IL increases the elasticity in the cholesterol-induced condensed phase of the lipid layer. The X-ray reflectivity measurement on a stack of cholesterol-free lipid bilayers proved the formation of IL-induced phase-separated domains in the matrix of a pure lipid phase. These domains are found to be formed by interdigitating the chains of the lipids, producing a thinner membrane. Such a phase is less intense in the cholesterol-containing membrane. All of these results indicate that the IL molecules may deform the cholesterol-free membrane of a bacterial cell, but the same may not be harmful to human beings as cholesterol could restrict the insertion in the cellular membrane of a human cell.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Cholesterol-Controlled Interaction of Ionic Liquids with Model Cellular Membranes

et al. 2023

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“…10 These changes lead to variations in the physiochemical properties, like viscosity, density, polarity, solute solubility, and many others. [11][12][13] Inorganic and organic synthesis and catalysis, 11,[14][15][16] extraction and separation, [17][18][19] nanomaterial synthesis, 20,21 food and bioproducts, 22 and pharmaceuticals [23][24][25] are the industries where ILs are useful. Analyzing the rheological behaviour of the aqueous solution of these ILs is fundamental in various commercial applications.…”

Section: Introductionmentioning

confidence: 99%

Temperature-dependent anomalous viscosity of aqueous solutions of imidazolium-based ionic liquids

et al. 2023

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Ionic liquids meet lipid bilayers: a state-of-the-art review

Benedetto

2023

Biophys Rev

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In the past 25 years, a vast family of complex organic salts known as room-temperature ionic liquids (ILs) has received increasing attention due to their potential applications. ILs are composed by an organic cation and either an organic or inorganic anion, and possess several intriguing properties such as low vapor pressure and being liquid around room temperature. Several biological studies flagged their moderate-to-high (cyto)-toxicity. Toxicity is, however, also a synonym of affinity, and this boosted a series of biophysical and chemical-physical investigations aimed at exploiting ILs in bio-nanomedicine, drug-delivery, pharmacology, and bio-nanotechnology. Several of these investigations focused on the interaction between ILs and lipid membranes, aimed at determining the microscopic mechanisms behind their interaction. This is the focus of this review work. These studies have been carried out on a variety of different lipid bilayer systems ranging from 1-lipid to 5-lipids systems, and also on cell-extracted membranes. They have been carried out at different chemical-physical conditions and by the use of a number of different approaches, including atomic force microscopy, neutron and X-ray scattering, dynamic light scattering, differential scanning calorimetry, surface quartz microbalance, nuclear magnetic resonance, confocal fluorescence microscopy, and molecular dynamics simulations. The aim of this “2023 Michèle Auger Award” review work is to provide the reader with an up-to-date overview of this fascinating research field where “ILs meet lipid bilayers (aka biomembranes),” with the aim to boost it further and expand its cross-disciplinary edges towards novel high-impact ideas/applications in pharmacology, drug delivery, biomedicine, and bio-nanotechnology.

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Thermodynamics and In-Plane Viscoelasticity of Anionic Phospholipid Membranes Modulated by an Ionic Liquid

Cited by 9 publications

References 75 publications

Cholesterol-Controlled Interaction of Ionic Liquids with Model Cellular Membranes

Cholesterol-Controlled Interaction of Ionic Liquids with Model Cellular Membranes

Temperature-dependent anomalous viscosity of aqueous solutions of imidazolium-based ionic liquids

Ionic liquids meet lipid bilayers: a state-of-the-art review

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