1996
DOI: 10.1006/jcht.1996.0099
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Thermodynamics of reactions catalyzed byL-iditol 2-dehydrogenase: the xylose assimilation pathway

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Cited by 7 publications
(6 citation statements)
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“… a A, B, P, Q relate to NAD(P)H, xylose, xylitol, and NAD(P) + , respectively b PO, values were found within a predefined range by parameter optimization (see main text and Additional file 1 : Table S3) c Values obtained from ligand-binding analysis using fluorescence spectroscopy (this study) d Values calculated from Haldane relationship K eq = V f 2 K iP K mQ /( V r 2 K iB K mA ) [ 38 ] e Equilibrium constants were calculated in accordance with the Haldane relationship [ K eq Haldane = V f K mP K iQ /( V r K mB K iA )] [ 38 ]. Values can be compared to those experimentally obtained in this study (CtXR) or to reported K eq exp [ 39 , 40 ]. Calculated Gibbs free energies of reaction of 6 ± 1 kJ mol −1 for the isomerization of xylose into xylulose [Δ G r = −RTln( K eqXR Haldane K eqXDH Haldane ] or Δ G r = −RTln( K eqXR exp K eqXDH exp ) are in excellence accordance with a value of 4.3 kJ mol −1 calculated from standard transformed Gibbs free energies of formation [ 41 ] f Reported kinetic parameters obtained from comprehensive full-kinetic study acquired at 25 °C in 50 mM potassium phosphate buffer pH 7.5 were applied [ 42 ].…”
Section: Resultsmentioning
confidence: 99%
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“… a A, B, P, Q relate to NAD(P)H, xylose, xylitol, and NAD(P) + , respectively b PO, values were found within a predefined range by parameter optimization (see main text and Additional file 1 : Table S3) c Values obtained from ligand-binding analysis using fluorescence spectroscopy (this study) d Values calculated from Haldane relationship K eq = V f 2 K iP K mQ /( V r 2 K iB K mA ) [ 38 ] e Equilibrium constants were calculated in accordance with the Haldane relationship [ K eq Haldane = V f K mP K iQ /( V r K mB K iA )] [ 38 ]. Values can be compared to those experimentally obtained in this study (CtXR) or to reported K eq exp [ 39 , 40 ]. Calculated Gibbs free energies of reaction of 6 ± 1 kJ mol −1 for the isomerization of xylose into xylulose [Δ G r = −RTln( K eqXR Haldane K eqXDH Haldane ] or Δ G r = −RTln( K eqXR exp K eqXDH exp ) are in excellence accordance with a value of 4.3 kJ mol −1 calculated from standard transformed Gibbs free energies of formation [ 41 ] f Reported kinetic parameters obtained from comprehensive full-kinetic study acquired at 25 °C in 50 mM potassium phosphate buffer pH 7.5 were applied [ 42 ].…”
Section: Resultsmentioning
confidence: 99%
“…e Equilibrium constants were calculated in accordance with the Haldane relationship [ K eq Haldane = V f K mP K iQ /( V r K mB K iA )] [ 38 ]. Values can be compared to those experimentally obtained in this study (CtXR) or to reported K eq exp [ 39 , 40 ]. Calculated Gibbs free energies of reaction of 6 ± 1 kJ mol −1 for the isomerization of xylose into xylulose [Δ G r = −RTln( K eqXR Haldane K eqXDH Haldane ] or Δ G r = −RTln( K eqXR exp K eqXDH exp ) are in excellence accordance with a value of 4.3 kJ mol −1 calculated from standard transformed Gibbs free energies of formation [ 41 ]…”
Section: Resultsmentioning
confidence: 99%
“…Among them, seven genes encoded the enzymes in xylose utilization pathway, including three alpha-xylosidases (xyl1, xyl2 and xyl3), two xylulose reductases (xdh1 and xdh2), a xylose reductase (xr1) and a xylulokinase (xylB), which are responsible for converting xylose to xylulose-5-P. In addition, the aldose 1-epimerase (galM) and L-iditol 2-dehydrogenase (sorD) genes, both involved in the interconversion between α-form and β-form sugars [ 36 ], and the zinc-dependent interconversion of polyols to their respective ketoses [ 37 ], respectively, were upregulated in the C5 cultures. These results revealed that the upregulated expression of a set of genes in the xylose utilization pathway and nonoxidative pentose phosphate pathway (PPP) might associate with serial transformantion and metabolic flow of xylose toward the glycolysis for generating metabolic energy and acetyl-CoA for cell growth and fatty acid biosynthesis, as summarized in Figure 2 .…”
Section: Resultsmentioning
confidence: 99%
“…The standard free energy change of the reaction for the steps involved in xylose catabolism has been determined from calorimetric measurements by Tewari and Goldberg (14), and the values are listed together with the calculated equilibrium constants in Table 1.…”
Section: Methodsmentioning
confidence: 99%