“…In natural conditions, sun light far exceeds the quantum requirements for photochemistry in photosynthesis, commonly referred as 'photoinhibition' (Misra, 1993;Misra et al 1997;2001;2007). Under these conditions, the PSII RC undergoes photoinduced damages of the D1 protein.…”
“…In natural conditions, sun light far exceeds the quantum requirements for photochemistry in photosynthesis, commonly referred as 'photoinhibition' (Misra, 1993;Misra et al 1997;2001;2007). Under these conditions, the PSII RC undergoes photoinduced damages of the D1 protein.…”
“…These samples were excited by a sequence of two flashes (General Radio, Stroboslave) with a halfdecay time of 3 ms (0.5 J) white light and quickly frozen to 77K by dropping them into a Dewar containing liquid nitrogen. TL glow curves were recorded as described by Misra et al (18,19). To inhibit electron transfer between Q A and Q B in the reaction centre of photosystem II, 5 mL 10 mmol/L DCMU was added to the chloroplast thylakoid sample before illumination.…”
Section: Measurement Of Thermoluminescencementioning
The thermoluminescence (TL) parameters in intact leaves and thylakoids isolated from leaves showed a different pattern of change during leaf ageing. Ageing of leaves brought about a decrease in the B-band and a simultaneous increase in the Q-band. Thylakoids showed only a decrease in the B-band. The TL bands further show that there is generation of an endogenous electron transport inhibitor during leaf ageing.
“…Photoinhibitory treatment which usually result in the decreased quantum efficiency of PSII and degradation of D1 polypeptide, do not affect the S-states but affects B-band with least effect on C-band (Misra et al, 1997;1998a). Isolated thylakoids showed a parallel decrease in Q-and B-band (Vass et al, 1988) suggesting both Q A and Q B is affected by photoinhibition.…”
Section: Stress Induced Changes In Tl Glow Peaksmentioning
confidence: 99%
“…Ohad et al (1990) reported a band shift of B-band to lower temperature, suggesting lumen acidification and redox changes in PSII. Misra et al (1997Misra et al ( , 1998a reported Q-and B-band intensity decreased with high temperature in pothos and spinach leaves. Janda et al (1999) reported membrane leakiness and grana destacking might be the reason for the changes in AG bands both at high temperature and freezing.…”
Section: Stress Induced Changes In Tl Glow Peaksmentioning
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