Thermophilic Actinomycetes from Hot Water Spring Capable of Producing Enzymes of Industrial Importance

Abstract

With the increasing demand for industrial enzymes with novel properties, the production of enzymes tolerant to extreme conditions has also increased. Due to their unique macromolecular properties, thermophiles possess high metabolic rates, physically and chemically stable enzymes and higher end product yields than similar mesophilic species. Actinomycetes are bacteria which require minimal nutritional requirement for their growth and hence are preferred over thermophilic bacteria as source of industrial enzymes. In this study, thermophilic actinomycetes were isolated from hot water springs situated at Vajreshwari near Mumbai, Maharashtra and screened for the production of cellulase and amylase. Five isolates of thermophilic actinomyces obtained, tested positive for both amylolytic and cellulolytic activity. Two out of the five isolates were chosen for large scale production of the enzyme. The enzyme activity of the crude extract was determined using DNSA assay for reducing sugar. The optimum conditions for activity of amylase from isolate no. 4 were 55 o C at pH 4.8 and for cellulase were 55 o C at pH 9. Amylase and cellulase with high stability and good activity at elevated temperature and acidic /alkaline conditions may find commercial applications in various fields such as such starch processing, food industries and detergents industries.

Detection of antibiotic-producing Actinobacteria in the sediment and water of Ma'in thermal springs (Jordan)

“…This agrees with the findings of previous Jordanian and some regional studies that showed Streptomyces as an important genus in such habitats. [22][23][24] Nevertheless, we report in this study the isolation of two additional actinobacterial species: Nocardiopsis sp. and Nocardioides luteus.…”

“…For instance, Chaudhary and Prabhu (2016) have described the presence of five different genera of Actinobacteria in sediment samples obtained from water springs in Mumbai, India. 24 These isolates belong to genera Streptomyces, Micromonospora, Actinomadura, Saccharomonospora, and Thermoactinomyces. 24 The isolated strains have a prominent enzymatic activity.…”

“…24 These isolates belong to genera Streptomyces, Micromonospora, Actinomadura, Saccharomonospora, and Thermoactinomyces. 24 The isolated strains have a prominent enzymatic activity. 24 Based on these studies, it can be concluded that the number and the type of Actinobacteria differ according to the sampling site, physical and chemical properties of the sampling sites, and the isolation strategy.…”

“…24 The isolated strains have a prominent enzymatic activity. 24 Based on these studies, it can be concluded that the number and the type of Actinobacteria differ according to the sampling site, physical and chemical properties of the sampling sites, and the isolation strategy. In our study, we were able to detect the frequently isolated genus Streptomyces with a clear antibacterial activity.…”