Thermophilic anaerobic digestion of winery effluents in a two-stage process and the effect of the feeding frequency on methane production

Vital‐Jácome, Miguel; Buitrón, Germán

doi:10.1016/j.chemosphere.2021.129865

Cited by 18 publications

(12 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The VFAs were subsequently consumed by methanogens as the anaerobic processes continued. Acetic acid had the highest concentration in the VFAs [16,20], and its fluctuation caused the variation of total VFA concentrations. Less frequent feeding led to wider fluctuations of pH and VFA concentrations during the feeding intervals [12].…”

Section: System Stabilitymentioning

confidence: 99%

“…The post-treatment reactor (R4) had an average CH 4 content and daily biogas production of 52.5% and 3.64 L•d −1 , respectively, which indicated that incompletely digested materials in digestate from R1, R2, and R3. Therefore, appropriately reducing the feeding frequency can increase the biogas performance of AD [20], and help meet the demand for "flexible gas production".…”

Section: Biogas Production and Ch 4 Contentmentioning

confidence: 99%

See 1 more Smart Citation

Effects of Feeding Regimes on Process Performance and Microbial Community Structure in Anaerobic Semi-continuously Stirred Tank Reactors Treating Corn Stover

Yuan

et al. 2021

Waste Biomass Valor

View full text Add to dashboard Cite

Corn stover is one of the dominant agricultural wastes in China, with high lignocellulose contents. Anaerobic digestion of corn stover converts biomass to biogas as sustainable energy. To improve the performance of anaerobic digestion, as well as for flexible biogas production, the effects of different feeding regimes on biogas production, system stability, and microbial community structure were investigated using laboratory-scale semi-continuously stirred tank reactors (semi-CSTRs) fed pretreated corn stover once a day (R1), every 2 days (R2) and every 3 days (R3) at an equivalent amount. The results showed that R3 and R2 produced 11.1% and 8.4% more methane, respectively. R3 correspondingly had higher lignocellulose conversion rates and system stability, followed by R2 and R1. Greater fluctuations in biogas, VFA concentrations and pH were found in less frequently fed reactors during the time interval between two feeds. Additionally, less feeding increased the relative abundance of Bacteroidetes and Firmicutes during hydrolysis and acidogenesis, and the dominant genus of archaea in all reactors was Methanobacterium, which is a hydrogenotrophic methanogen. Therefore, appropriately reducing the feeding frequency can increase the process performance of anaerobic digestion of corn stover.

show abstract

Section: System Stabilitymentioning

confidence: 99%

Section: Biogas Production and Ch 4 Contentmentioning

confidence: 99%

Effects of Feeding Regimes on Process Performance and Microbial Community Structure in Anaerobic Semi-continuously Stirred Tank Reactors Treating Corn Stover

Yuan

et al. 2021

Waste Biomass Valor

View full text Add to dashboard Cite

show abstract

“…Finally, condition 4 consisted of the same con guration and temperatures as condition 3 but with an increment in the organic loading rate (OLR) for both reactors (A4-M4) (Table 1). The start-up conditions and the acidogenic and methanogenic performances were previously reported [3,7]. The single-stage system (M1) and the acidogenic reactors (A2, A3, and A4) were fed with untreated winery e uents.…”

Section: Anaerobic Digestion Systemmentioning

confidence: 99%

“…The rst effect of this change in operating conditions was a signi cant increase in methane production. However, after this increase in productivity, it was reported that the process suffered instability and overloading, gradually reducing the process e ciency [7]. Therefore, it can be inferred that applying harsher operating conditions affects the resilience and stability of the process through the loss of microbial communities.…”

Section: Taxonomic Pro Le M3 Vs M4mentioning

confidence: 99%

“…Methane [4] and other products such as biohydrogen [5] or medium-chain carboxylic acids [6] can also be obtained. Recently, highly concentrated winery e uents were successfully treated to produce methane in single-stage and two-stage (acidogenic and methanogenic) reactors, obtaining better performance in the two-stage con guration and reaching higher productivity in thermophilic than in mesophilic conditions [3,7].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Metabolic functional profiles of microbial communities in methane production systems treating winery wastewater

Vital‐Jácome

Carrillo‐Reyes

Buitrón

2023

Preprint

Self Cite

View full text Add to dashboard Cite

Winery effluents are agro-industrial wastes that can be treated to produce methane in anaerobic digestion systems. Understanding how the process configuration and operating conditions affect the microbial communities and their metabolism is essential to improve the performance of these systems. This work aimed to compare the taxonomic and functional profiles of four process configurations of an anaerobic digestion system treating winery effluents. Taxonomic profiles were obtained by sequencing the 16S rRNA gene, and the Tax4Fun2 package was used to predict the functional profiles. Different genera predominated under three configurations in the acidogenic reactor, Sporolactobacillus, Prevotella and Acetobacter, respectively; however, the analysis indicated high functional redundancy among configurations. Methane production by the acetoclastic pathway was mainly related to Methanosaeta in the single-stage configuration and related to five different archaea genera in the two-stage configuration. Syntrophic acetate oxidation coupled to hydrogenotrophic methanogenesis predominated under thermophilic conditions, where Methanothermobacter had up to 65% of the relative abundance. The two-stage configuration was more stable due to the higher microbial diversity and functional redundancy than the single-stage; the microbial community in mesophilic conditions also had higher microbial diversity and functional redundancy than in thermophilic conditions; finally, applying harsher operating conditions reduces the microbial diversity and the stability of the process. This analysis provided valuable knowledge to understand and improve methane production in anaerobic digestion systems.

show abstract

Metabolic Functional Profiles of Microbial Communities in Methane Production Systems Treating Winery Wastewater

2023

Self Cite

View full text Add to dashboard Cite

This study investigated the impact of process configuration and conditions on microbial communities and metabolic pathways in the anaerobic digestion of winery effluents. Four system configurations were analyzed for taxonomic and functional profiles using 16S rRNA gene sequencing and Tax4Fun2. Sporolactobacillus, Prevotella, and Acetobacter dominated (> 70%) in the acidogenic reactor with 5277 conserved functions across configurations. In the methanogenic reactor, methane production relied on Methanosaeta in the single-stage configuration (13%) and five archaea genera in the two-stage configuration (18%). Thermophilic conditions favored syntrophic acetate oxidation and hydrogenotrophic methanogenesis by Methanothermobacter (65%), significantly changing due to temperature. The two-stage configuration exhibited 3.0 times higher functional redundancy than the single-stage configuration. Mesophilic conditions displayed 2.5 times greater functional redundancy than thermophilic conditions. High organic loading rate and short hydraulic retention time reduced functional redundancy by 1.5 times. Assessing microbial functionality beyond their composition is crucial to understand stability and performance of anaerobic digestion systems.

show abstract

Thermophilic anaerobic digestion of winery effluents in a two-stage process and the effect of the feeding frequency on methane production

Cited by 18 publications

References 39 publications

Effects of Feeding Regimes on Process Performance and Microbial Community Structure in Anaerobic Semi-continuously Stirred Tank Reactors Treating Corn Stover

Effects of Feeding Regimes on Process Performance and Microbial Community Structure in Anaerobic Semi-continuously Stirred Tank Reactors Treating Corn Stover

Metabolic functional profiles of microbial communities in methane production systems treating winery wastewater

Metabolic Functional Profiles of Microbial Communities in Methane Production Systems Treating Winery Wastewater

Contact Info

Product

Resources

About