Thermophilic Bacteria in Lake Baikal Bottom Sediments Associated with Hydrocarbon Discharge

Pavlova, Olga; Lomakina, A. V.; Novikova, Angelina S.; Chernitsyna, S. M.; Khanaeva, T. A.; Pogodaeva, T. V.; Khabuev, Andrey V.; Zemskaya, Т. I.

doi:10.1134/s0026261719030081

Cited by 6 publications

(2 citation statements)

References 31 publications

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“…Thermoanaerobacteraceae were found at high levels in TYC enrichment cultures containing S 0 , SO 4 2and S 2 O 3 2-, consistent with the observation that members of this family are generally S 0 and S 2 O 3 2reducers [69][70][71]. A member of the Thermaerobacteraceae, a family of mostly aerobic organisms, was found at 6.7% in one of the three S 0 /oil enrichment cultures and at low but consistent levels throughout the other cultures, consistent with the finding of anaerobic examples within this family [72,73]. While the finding of the Thermaerobacteraceae observed here in an enrichment containing S 0 suggests it may be capable of sulfur reduction, we have classified it as a general organotroph for a lack of direct evidence of this metabolism.…”

Section: Sulfate Thiosulfate and Sulfur-reducing Organoheterotrophssupporting

confidence: 88%

Characterization of the microbiomes from Salton Sea Trough mud volcanoes, seeps, and enrichment cultures: predominance of H2-autotrophy, H2-producing organotrophy, acetate cycling, and sulfur reduction

Dowling

Leshchinskiy

et al. 2023

Preprint

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The Salton Sea Trough has a range of geothermal features, including mud volcanoes, pots, and seeps that express fluids at temperatures ranging from ambient to 65 to 100 C. The features produce 99% CO2/0.5% CH4 gas of a thermogenic origin and contain hydrothermally-produced petroleum. The mud/clay from the Davis-Schrimpf mud volcanoes has an elemental sulfur (S0) concentration of 317 microM, suggesting that sulfur metabolism should be important in the system. Surprisingly, there has been very little microbiological characterization of these features. Described here are microbial communities, determined by Illumina sequencing of the 515F – 806R 16S rRNA gene fragment, from mud volcanoes/seeps from the Davis-Schrimph seep field and nearby areas with surface feature temperatures from ambient, 65 C, and 100 C. In addition, we have characterized the communities that developed over 1 month at 65 C in enrichment cultures from the 65 C mud volcanoes incubated with a range of electron acceptors (ferrihydrite, S0, SO42-, and S2O32-) and electron donors (tryptone/yeast/casamino acids, crude oil, and CH3CO2-). The 65 C mud and enrichment culture communities were generally predominated by autotrophic H2-oxidizers and organotrophic H2-producers, acetogens, acetotrophs and heterotrophic S0, SO42-, and S2O32- reducers, including many relatives of microbes previously observed in the deep subsurface and from petroleum fields or production waters. While methanogens were present, they were generally at low levels, and few obvious methylotrophs or anaerobic methane oxidizers were detected. Overall, these results provide evidence for a subsurface lithoautotrophic microbial ecosystem (or SliME) in the Salton Sea trough subsurface.

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Section: Sulfate Thiosulfate and Sulfur-reducing Organoheterotrophssupporting

confidence: 88%

Characterization of the microbiomes from Salton Sea Trough mud volcanoes, seeps, and enrichment cultures: predominance of H2-autotrophy, H2-producing organotrophy, acetate cycling, and sulfur reduction

Dowling

Leshchinskiy

et al. 2023

Preprint

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“…In the process of microbial communities from Lake Baikal fault zones cultivation, we have isolated several pure cultures of thermophilic bacteria with unusual for their species metabolism. Unlike cold marine sediments where endospores of thermophiles belong to obligate anaerobes, mantaining their metabolism via fermentation of organic substrates or sulphate reduction (Hubert et al, 2010;Müller et al, 2014;Chakraborty et al, 2018), in Baikal sediments associated with seepages of gas-saturated fluids we found facultatively anaerobic thermophilic prokaryotes of the genera Paracoccus, Geobacillus and Thermaerobacter (Pavlova et al, 2016;Khanaeva et al, 2017;Pavlova et al, 2019b). Genomic studies confirmed the potential of Thermaerobacter PB12/4term pure culture for mixotrophic type of nutrition, which is uncharacteristic for the members of genus Thermaerobacter as obligate aerobes (Baturina et al, 2018).…”

Section: Resultsmentioning

confidence: 65%

Experimental equipment for Lake Baikal deep biosphere microorganism’s exploration and some results obtained using this equipment

Pavlova

Manakov

Bukin

et al. 2019

LFWB

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The development of special autoclaves with automatic temperature control (Nikolaev Institute of Inorganic Chemistry) had enabled reproduction of Lake Baikal tectonically active zone typical conditions (80°C, 5 MPa). This article describes in details the equipment developed and demonstrates some results obtained using the equipment. In series of thermobaric experiments on the cultivation of bottom sediment microbial communities, we have determined the microbial community potential to transform organic matter via the formation of dibenzothiophenes, tri-and monoaromatic steroids as well as petroleum biomarkers (retene and gammacerene). The particular bottom sediment geochemical environment associated with hydrocarbon discharge imply the composition of microbial communities and, hence, the organic matter transformation degree as well as a compound spectrum resulting from the destruction of organic matter under thermobaric conditions. The presence of microorganisms with an unusual metabolism suggests the promising potential for such studies both in application to Lake Baikal and worldwide

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Description and Genomic Analysis of the First Facultatively Lithoautotrophic, Thermophilic Bacteria of the Genus Thermaerobacter Isolated from Low-temperature Sediments of Lake Baikal

et al. 2023

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Thermophilic Bacteria in Lake Baikal Bottom Sediments Associated with Hydrocarbon Discharge

Cited by 6 publications

References 31 publications

Characterization of the microbiomes from Salton Sea Trough mud volcanoes, seeps, and enrichment cultures: predominance of H2-autotrophy, H2-producing organotrophy, acetate cycling, and sulfur reduction

Characterization of the microbiomes from Salton Sea Trough mud volcanoes, seeps, and enrichment cultures: predominance of H2-autotrophy, H2-producing organotrophy, acetate cycling, and sulfur reduction

Experimental equipment for Lake Baikal deep biosphere microorganism’s exploration and some results obtained using this equipment

Description and Genomic Analysis of the First Facultatively Lithoautotrophic, Thermophilic Bacteria of the Genus Thermaerobacter Isolated from Low-temperature Sediments of Lake Baikal

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