Thermophilic bacterium Caldimonas taiwanensis produces poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from starch and valerate as carbon sources

Sheu, Der-Shyan; Chen, Wen-Ming; Yang, Jr-Yung; Chang, Rey-Chang

doi:10.1016/j.enzmictec.2009.01.004

Cited by 74 publications

(34 citation statements)

References 21 publications

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“…Few reports have mentioned thermophilic PHA synthases (14). In these limited reports, thermophiles exhibited a more efficient ability for PHA accumulation than mesophiles (16,17,31). The results of those studies also support the assumption that thermophilic PHA synthase has a higher level of enzyme activity.…”

supporting

confidence: 78%

Mutations Derived from the Thermophilic Polyhydroxyalkanoate Synthase PhaC Enhance the Thermostability and Activity of PhaC from Cupriavidus necator H16

Sheu¹,

Chen²,

Lai³

et al. 2012

Journal of Bacteriology

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supporting

confidence: 78%

Mutations Derived from the Thermophilic Polyhydroxyalkanoate Synthase PhaC Enhance the Thermostability and Activity of PhaC from Cupriavidus necator H16

Sheu¹,

Chen²,

Lai³

et al. 2012

Journal of Bacteriology

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“…2003, 2005). Very recently, the thermophilic bacterium Caldimonas taiwanensis was investigated for poly(3HB‐ co ‐3HV) accumulation at 50°C (Sheu et al. 2009).…”

Section: Discussionmentioning

confidence: 99%

“…Regardless of the low cell density and low polymer productivity, high optimum temperatures were reported for the PHA synthase (70°C) and the b-ketothiolase (65°C) of the thermophilic bacterium Thermus thermophilus HB8 (Pantazaki et al 2003(Pantazaki et al , 2005. Very recently, the thermophilic bacterium Caldimonas taiwanensis was investigated for poly(3HB-co-3HV) accumulation at 50°C (Sheu et al 2009). Its growth and polymer productivity were significantly enhanced when yeast extract was supplemented to the cultivation medium.…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of new poly(3HB)-accumulating star-shaped cell-aggregates-forming thermophilic bacteria

Ibrahim

Willems

Steinbüchel

2010

Journal of Applied Microbiology

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Aims: This study aimed at isolating thermophilic bacteria that utilize cheap carbon substrates for the economically feasible production of poly(3-hydroxybutyrate), poly(3HB), at elevated temperatures. Methods and Results: Thermophilic bacteria were enriched from an aerobic organic waste treatment plant in Germany, and from hot springs in Egypt. Using the viable colony staining method for hydrophobic cellular inclusions with Nile red in mineral salts medium (MSM) containing different carbon sources, six Gram-negative bacteria were isolated. Under the cultivation conditions used in this study, strains MW9, MW11, MW12, MW13 and MW14 formed stable star-shaped cell-aggregates (SSCAs) during growth; only strain MW10 consisted of free-living rod-shaped cells. The phylogenetic relationships of the strains as derived from 16S rRNA gene sequence comparisons revealed them as members of the Alphaproteobacteria. The 16S rRNA gene sequences of the isolates were very similar (> 99% similarity) and exhibited similarities ranging from 93 to 99% with the most closely related species that were Chelatococcus daeguensis, Chelatococcus sambhunathii,Chelatococcus asaccharovorans, Bosea minatitlanensis, Bosea thiooxidans and Methylobacterium lusitanum. Strains MW9, MW10, MW13 and MW14 grew optimally in MSM with glucose, whereas strains MW11 and MW12 preferred glycerol as sole carbon source for growth and poly(3HB) accumulation. The highest cell density and highest poly(3HB) content attained were 4 center dot 8 g l-l (cell dry weight) and 73% (w/w), respectively. Cells of all strains grew at temperatures between 37 and 55 degrees C with the optimum growth at 50 degrees C. Conclusions: New PHA-accumulating thermophilic bacterial strains were isolated and characterized to produce poly(3HB) from glucose or glycerol in MSM at 50 degrees C. SSCAs formation was reported during growth. Significance and Impact of the Study: To the best of our knowledge, this is the first report on the formation of SSCAs by PHA-accumulating bacteria and also by thermophilic bacteria. PHA-producing thermophiles can significantly reduce the costs of fermentative PHA production

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“…The authors proposed its classification as Caldimonas taiwanensis sp. nov. Later, Sheu et al [104] detected that, under nitrogen-limited conditions, C. taiwanensis produces PHB at 55 °C from common carbon sources such as fructose, gluconate, glycerol, and maltose, but not from fatty acids if provided as sole carbon source. PHBHV copolyester production occurred by co-feeding a mix of gluconate and valerate.…”

Section: Thermophile Pha Producersmentioning

confidence: 99%

Production of Polyhydroxyalkanoate (PHA) Biopolyesters by Extremophiles?

Koller¹

2017

MOJPS

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The article reviews the current state of knowledge of the production of polyhydroxyalkanoate (PHA) biopolyesters under extreme environmental conditions.Although PHA production by extremophiles is not realized yet at industrial scale, significant PHA accumulation under high salinity or extreme pH-or temperature conditions was reported for diverse representatives of the microbial domains of both Archaea and Bacteria. Several mechanisms were proposed to explain the mechanistic role of PHA and their monomers as microbial cell-and enzyme protective chaperons and the factors boosting PHA biosynthesis under environmental stress conditions. The potential of selected extremophile strains, isolated from extreme environments like glaciers, hot springs, saline brines, or from habitats highly polluted with heavy metals or solvents, for efficient future PHA production on an industrially relevant scale is assessed based on the basic data available in the scientific literature. The article reveals that, beside the needed optimization of other cost-decisive factors like inexpensive raw materials or efficient downstream processing, the application of extremophile production strains can drastically safe energy costs, are easily accessible towards long-term cultivation in chemostat processes, and therefore might pave the way towards cost-efficient PHA production, even combined with safe disposal of industrial waste streams. However, further challenges have still to be overcome in terms of strain improvement and process engineering aspects.

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Thermophilic bacterium Caldimonas taiwanensis produces poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from starch and valerate as carbon sources

Cited by 74 publications

References 21 publications

Mutations Derived from the Thermophilic Polyhydroxyalkanoate Synthase PhaC Enhance the Thermostability and Activity of PhaC from Cupriavidus necator H16

Mutations Derived from the Thermophilic Polyhydroxyalkanoate Synthase PhaC Enhance the Thermostability and Activity of PhaC from Cupriavidus necator H16

Isolation and characterization of new poly(3HB)-accumulating star-shaped cell-aggregates-forming thermophilic bacteria

Production of Polyhydroxyalkanoate (PHA) Biopolyesters by Extremophiles?

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