Thermoplastic microfluidic devices for targeted chemical and biological applications

Voicu, Dan; Lestari, Gabriella; Wang, Yihe; Debono, Michael; Seo, Minseok; Cho, Sangho; Kumacheva, Eugenia

doi:10.1039/c6ra27592c

Cited by 30 publications

(16 citation statements)

References 31 publications

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“…In order to simplify fabrication processes and adapt them for industrial applications that require rapid fabrication of multilayered microfluidic devices with good performance, different materials have been developed for the applications in microfluidics. Additional polymers have been identified as complementary to PDMS in terms of transparency, biocompatibility and flexibility, but with higher rigidity and better resistance to solvents such as: thermoplastic elastomers [ 139 ], thermoset polyester [ 140 ], polyurethane methacrylate [ 141 ], and Norland Adhesive 81 [ 142 ]. Paper-based substrates provide an alternative to expensive polymers due to low-cost and ease of acquisition, biocompatibility, and availability of papers with different physical properties (porosity, thickness, capillary flow rate, etc.).…”

Section: Microfluidicsmentioning

confidence: 99%

Point-of-Need DNA Testing for Detection of Foodborne Pathogenic Bacteria

Vidić

Vizzini

Manzano

et al. 2019

Sensors

104

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Foodborne pathogenic bacteria present a crucial food safety issue. Conventional diagnostic methods are time-consuming and can be only performed on previously produced food. The advancing field of point-of-need diagnostic devices integrating molecular methods, biosensors, microfluidics, and nanomaterials offers new avenues for swift, low-cost detection of pathogens with high sensitivity and specificity. These analyses and screening of food items can be performed during all phases of production. This review presents major developments achieved in recent years in point-of-need diagnostics in land-based sector and sheds light on current challenges in achieving wider acceptance of portable devices in the food industry. Particular emphasis is placed on methods for testing nucleic acids, protocols for portable nucleic acid extraction and amplification, as well as on the means for low-cost detection and read-out signal amplification.

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Section: Microfluidicsmentioning

confidence: 99%

Point-of-Need DNA Testing for Detection of Foodborne Pathogenic Bacteria

Vidić

Vizzini

Manzano

et al. 2019

Sensors

104

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“…In our study, we did not determine the actual concentrations of drug (APAP) used for testing in microfluidic devices, but the data showing drug dose‐dependent cellular responses (Figure f,g) indicate that drug absorption to PDMS may not be significant to influence the results in our drug testing. However, several strategies to avoid drug absorption issue of PDMS (e.g., surface modification, device fabrication with thermoplastics) could be considered to further improve the performance of our microfluidic systems for organoid experiments …”

Section: Resultsmentioning

confidence: 99%

Vascularized Liver Organoids Generated Using Induced Hepatic Tissue and Dynamic Liver‐Specific Microenvironment as a Drug Testing Platform

Jin

Kim

Lee

et al. 2018

Adv Funct Materials

118

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Induced hepatic (iHep) cells generated by direct reprogramming have been proposed as cell sources for drug screening and regenerative medicine. However, the practical use of a 3D hepatic tissue culture comprised of iHep cells for drug screening and toxicology testing has not been demonstrated. In this study, a 3D vascularized liver organoid composed of iHep cells and a decellularized liver extracellular matrix (LEM) cultured in a microfluidic system is demonstrated. iHep cells are generated by transfection with polymer nanoparticles and plasmids expressing hepatic transcription factors. The iHep cells are cocultured with endothelial cells in the 3D LEM hydrogel in a microfluidic-based cell culture device with a continuous dynamic flow of media. The resultant 3D vascularized liver organoids maintained under this physiologically relevant culture microenvironment exhibit improved hepatic functionalities, metabolic activity, biosynthetic activity, and drug responses. Finally, the feasibility of using the iHep-based 3D liver organoid as a highthroughput drug screening platform, as well as its use in a multiorgan model comprised of multiple internal organoids is confirmed. The study suggests that a combined strategy of direct reprogramming, matrix engineering, and microfluidics can be used to develop a highly functional, standardized, drug screening, and toxicological analysis platform.

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“…Bu gelişmeyle birlikte, mikro-akışkan çiplerde yaygın olarak kullanıldı ve günümüzde de kullanılmaya devam edilmektedir. İlerleyen yıllarda hücre ve proteinleri ayırma modelleme de, biyosensörler, kültür ve araştırmalar gibi biyolojik uygulamalarda mikro-akışkan cihazlar kullanılmıştır [10][11][12].…”

Section: Introductionunclassified

Mems Tabanli Mi̇kro-Akişkan Çi̇pi̇n Zamana Bağli Basinç Anali̇zi̇

Ülkir

2020

International Journal of 3D Printing Technologies and Digital Industry

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Mikro elektromekanik sistemler (MEMS), ölçülen bir mekanik sinyalin makine tarafından okunabilir bir sinyale dönüştürülmesini sağlayan mekanik ve elektromekanik mikro ölçekte bileşenlerden oluşan bir teknolojidir. Geleneksel mekanik üretimin aksine, MEMS cihazının üretiminde, entegre bir devre ile uyumlu olan yüzey mikroişleme ve toplu mikroişleme süreçlerini içeren yarı iletken yöntemi kullanılır. Bu aygıtlar veya sistemler makro ölçekteki etkileri algılama, denetleme, etkinleştirme ve oluşturma yeteneğine sahiptir. Bu çalışmada, mikro litre ve daha küçük hacimlerdeki akışkanların mikro ölçekteki kanallar içerisinde kontrol edilmesini ve hareket etmesini sağlayan bir sistem olan mikro-akışkan çipin tasarımı 3B tasarım programı kullanılarak gerçekleştirilmiştir. Mikro-akışkan, tek kanal girişli ve dört kanal çıkışlı olacak biçimde tasarlanmıştır. Bu mikro-akışkanın ön fiziksel testleri, araştırılması ve zamana bağlı basınç analizleri COMSOL MultiPhysics programı ile yapılmıştır. Tasarımı gerçekleştirilen mikro-akışkan çipin t=0, 0.5, 1, 1.5 ve 2 s zaman değerlerine bağlı akış yönü ve basınç analizi yapılmıştır. Analiz sonucunda mikro-akışkan kanallarında zamana bağlı akış yönü ve basınç değerleri farklılık göstermiştir.

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Thermoplastic microfluidic devices for targeted chemical and biological applications

Abstract: Photolithography and hot embossing offers the capability of cost-efficient and high-fidelity fabrication of polymer microfluidic devices.

Cited by 30 publications

References 31 publications

Point-of-Need DNA Testing for Detection of Foodborne Pathogenic Bacteria

Point-of-Need DNA Testing for Detection of Foodborne Pathogenic Bacteria

Vascularized Liver Organoids Generated Using Induced Hepatic Tissue and Dynamic Liver‐Specific Microenvironment as a Drug Testing Platform

Mems Tabanli Mi̇kro-Akişkan Çi̇pi̇n Zamana Bağli Basinç Anali̇zi̇

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