Thermopriming-associated proteome and sugar content responses in Pinus radiata embryogenic tissue

Castander-Olarieta, Ander; Pereira, Cátia; Mendes, Vera M.; Correia, Sandra; Manadas, Bruno; Canhoto, Jorge; Montalbán, Itziar A.; Moncaleán, Paloma

doi:10.1016/j.plantsci.2022.111327

Cited by 7 publications

(6 citation statements)

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“…Notwithstanding that the genome of P. halepensis is yet to be fully sequenced and may have negatively influenced the identification numbers, we were able to identify 1315 proteins and determine the relative concentration for 858. This is in agreement with the average number found in other proteomic studies in Pinus species [ 31 , 37 , 38 , 39 ].…”

Section: Discussionsupporting

confidence: 93%

“…The histone H4 variant TH091 and histone H2A.2.2 (core components of nucleosomes), and the 40S ribosomal protein S15a-1 and 60S ribosomal protein L18-3 (structural components of ribosomes) had increased amounts in samples from heat stress conditions. This agrees with previous studies on the temperature stress response, where ribosomal proteins, translation regulator factors and translation regulatory proteins played a significant role [ 39 , 64 , 65 , 66 ]. This data, combined with the fact that proteins´ abundance variations are not always correlated with changes in the corresponding transcriptome [ 67 ], can explain the lack of significant differences found in the transcription assay performed here.…”

Section: Discussionsupporting

confidence: 93%

“…As reviewed by Pinheiro et al [ 40 ], several proteome studies developed in Mediterranean woody species for the identification of stress-responsive proteins and tolerance/adaptation mechanisms under stress revealed high metabolic adjustment. Furthermore, in agreement with the data presented here, the rearrangement of various basal and secondary metabolic pathways such as glycolysis, the tricarboxylic acid cycle and secondary metabolites production has been found in different pine species as a general stress response [ 20 , 31 , 37 , 39 , 41 , 42 , 43 ].…”

Section: Discussionsupporting

confidence: 91%

“…A parallel analysis of the proteome of EMs induced under heat stress (40 °C for 4 h and 60 °C for 5 min) of radiata pine was developed by our group [ 39 ]. The results found from the GO analysis showed the same over-representation of proteins regarding their biological processes (response to cadmium ion, proteasome mediated ubiquitin-dependent protein catabolic process, the glycolytic process and the tricarboxylic acid cycle); likewise, a deep reorganization of the machinery involved in protein synthesis, folding, transport and degradation was found.…”

Section: Discussionmentioning

confidence: 99%

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Proteomic and Metabolic Analysis of Pinus halepensis Mill. Embryonal Masses Induced under Heat Stress

Pereira

Castander-Olarieta

Montalbán

et al. 2023

IJMS

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Understanding the physiological and molecular adjustments occurring during tree stress response is of great importance for forest management and breeding programs. Somatic embryogenesis has been used as a model system to analyze various processes occurring during embryo development, including stress response mechanisms. In addition, “priming” plants with heat stress during somatic embryogenesis seems to favor the acquisition of plant resilience to extreme temperature conditions. In this sense, Pinus halepensis somatic embryogenesis was induced under different heat stress treatments (40 °C for 4 h, 50 °C for 30 min, and 60 °C for 5 min) and its effects on the proteome and the relative concentration of soluble sugars, sugar alcohols and amino acids of the embryonal masses obtained were assessed. Heat severely affected the production of proteins, and 27 proteins related to heat stress response were identified; the majority of the proteins with increased amounts in embryonal masses induced at higher temperatures consisted of enzymes involved in the regulation of metabolism (glycolysis, the tricarboxylic acid cycle, amino acid biosynthesis and flavonoids formation), DNA binding, cell division, transcription regulation and the life-cycle of proteins. Finally, significant differences in the concentrations of sucrose and amino acids, such as glutamine, glycine and cysteine, were found.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Proteomic and Metabolic Analysis of Pinus halepensis Mill. Embryonal Masses Induced under Heat Stress

Pereira

Castander-Olarieta

Montalbán

et al. 2023

IJMS

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“…In our lab, several studies have been developed to optimize somatic embryogenesis [ 18 ] and organogenesis [ 19 , 20 ] in many species of Pinus . Moreover, as a result of priming cells during the somatic embryogenesis process in conifers, different metabolic, epigenetic, and proteomic profiles related to abiotic stress tolerance have been described recently [ 21 , 22 , 23 ]. However, as far as we know, no work has been done in order to improve the micropropagation of P. ponderosa by testing the chemical composition of the culture media or physical factors, such as type of lights (fluorescent versus LEDs) and their effect on morphogenesis, tissue micromorphology, and the overall success of the process.…”

Section: Introductionmentioning

confidence: 99%

Testing Explant Sources, Culture Media, and Light Conditions for the Improvement of Organogenesis in Pinus ponderosa (P. Lawson and C. Lawson)

Rojas-Vargas

Castander-Olarieta²,

Nascimento³

et al. 2023

Plants

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Pinus. ponderosa (P. Lawson and C. Lawson) is a commercial tree and one of the most important forest species in North America. Ponderosa pine suffers hardship when going through vegetative propagation and, in some cases, 15–30 years are needed to achieve full reproductive capacity. Based on previous works on P. ponderosa regeneration through in vitro organogenesis and trying to improve the published protocols, our objective was to analyze the influence of different types of explants, basal culture media, cytokinins, auxins, and light treatments on the success of shoot multiplication and rooting phases. Whole zygotic embryos and 44 µΜ 6-benzyladenine showed the best results in terms of explants survival. For shoot organogenesis, whole zygotic embryos and half LP (LP medium, Quoirin and Lepoivre, 1977, modified by Aitken-Christie et al., 1988) macronutrients were selected. A significant positive interaction between whole zygotic embryos and half LP macronutrients was found for the percentage of explants forming shoots. Regarding the light treatments applied, a significantly higher percentage of shoots elongated enough to be rooted was detected in shoots growing under blue LED at a light intensity of 61.09 µmol m−2 s−1. However, the acclimatization percentage was higher in shoots previously cultivated under fluorescent light at a light intensity of 61.71 µmol m−2 s−1. Anatomical studies using light microscopy and scanning electron microscopy showed the light treatments promoted differences in anatomical aspects in in vitro shoots; needles of plantlets exposed to red and blue LEDs revealed less stomata compared with needles from plantlets exposed to fluorescent light.

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Genetic stability, amino acid, and polyamine profile analyses in radiata pine somatic embryos maturated at high temperatures

Montalbán,

Castander-Olarieta,

do Nascimento

et al. 2024

Plant Cell Tiss Organ Cult

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Applying stress factors such as high temperatures during the different stages of somatic embryogenesis is either important interesting to uncover the molecular mechanisms involved in stress response and adaptation, and as a strategy to produce plants adapted to harsh environmental conditions derived from climate changes. In this sense, the present work aims to study the effect of high temperatures applied during maturation of somatic embryogenesis in the ploidy stability, the amino acid and polyamine pro les of the somatic embryos obtained and in the morphological characteristics of the somatic plantlets. The results revealed that the maturation temperature did not affect the morphology of the resulting somatic plantlets, neither the ploidy and genome size of phenotypically normal somatic embryos, whose ploidy and DNA content levels were similar to those found in mature zygotic embryos.Nonetheless, a slight but signi cant reduction of the genome size of aberrant somatic embryos was observed. Of the 21 amino acids detected signi cant differences depending on the maturation temperature were found for glycine, arginine, lysine and ornithine. These last three amino acids are precursors of the polyamines detected. Regarding this, putrescine levels were higher in somatic embryos from the highest maturation temperature (5 min pulse at 60 ºC), however the amount of this polyamine in all samples was much lower than spermidine, spermine and cadaverine. In conclusion, the different temperatures applied did not led to substantial changes in the ploidy level, endogenous PAs of the somatic embryos developed, or in the morphology of the somatic plantlets. Signi cant changes in the endogenous amino acids were observed, which may be linked not only to PAs metabolism but to another metabolic pathways involved in stress response. Key MessageMaturation temperature doesn´t affect ploidy levels of radiata pine somatic embryos, however, it affects the endogenous levels of glycine, arginine, lysine and putrescine.

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Thermopriming-associated proteome and sugar content responses in Pinus radiata embryogenic tissue

Cited by 7 publications

References 74 publications

Proteomic and Metabolic Analysis of Pinus halepensis Mill. Embryonal Masses Induced under Heat Stress

Proteomic and Metabolic Analysis of Pinus halepensis Mill. Embryonal Masses Induced under Heat Stress

Testing Explant Sources, Culture Media, and Light Conditions for the Improvement of Organogenesis in Pinus ponderosa (P. Lawson and C. Lawson)

Genetic stability, amino acid, and polyamine profile analyses in radiata pine somatic embryos maturated at high temperatures

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