Thermoresponsive block copolymer brush for temperature-modulated hepatocyte separation

Abstract

Hepatic tissue engineering may be an effective approach for the treatment of liver disease; however, its practical application requires hepatic cell separation technologies that do not involve cell surface modification...

Temperature-modulated separation of vascular cells using thermoresponsive-anionic block copolymer-modified glass

“…By reducing the temperature to 20 °C, adhered HUVECs were detached from PAAc-PN-750, whereas SMCs maintained their adherence to the brush. Previous reports have suggested that cell adhesion behavior differs between coculture and monoculture conditions, which has been attributed to the secreted extracellular matrix under coculture conditions or the reduced activity of cells after the application of cell-staining reagents [ 70 , 94 ]. However, similar cell adhesion behavior was observed in HUVECs and SMCs under both co-culture and monoculture conditions ( Fig.…”

“…Moreover, PNIPAAm exhibits extension and shrinkage below and above the transition temperature, respectively. The unique properties of PNIPAAm have been applied in various biomedical contexts, such as temperature-regulated drug and gene delivery systems [ [36] , [37] , [38] , [39] , [40] , [41] ], bioanalysis and biosensor devices [ [42] , [43] , [44] , [45] , [46] , [47] ], nano-actuators [ [48] , [49] , [50] , [51] ], bioseparation tools [ [52] , [53] , [54] , [55] , [56] , [57] , [58] , [59] , [60] , [61] , [62] , [63] ], cell-separation materials [ [32] , [33] , [34] , [64] , [65] , [66] , [67] , [68] , [69] , [70] ], cell culture substrates [ [71] , [72] , [73] , [74] , [75] , [76] , [77] ], and cell sheet therapy in diverse types of regenerative medicine [ 5 , [78] , [79] , [80] , [81] , [82] , [83] , [84] , [85] , [86] , [87] , [88] ]. Temperature-controlled cell adhesion and detachment were applied to cell separation procedures using PNIPAAm-modified substrates.…”

“…Block copolymers composed of upper thermoresponsive polymer segments and lower cell-affinity polymer segments on substrates have proven to be effective polymer-modified structures for temperature-modulated cell separation [ 67 , 70 ]. In the polymer-modified substrate, the upper PNIPAAm dehydrates and shrinks at 37 °C, leading to cell adhesion attributed to the enhanced interaction between cells and the lower affinity polymer segment.…”

Thermo-responsive block copolymers: assembly and application

“…Up to now, applications of thermo-responsive block copolymers have mostly focused on biomedical fields, such as imaging and drug delivery. [88][89][90][91][92] In this review, we mainly discuss their applications in other fields in conjunction with their properties, based on the structure of thermo-responsive block copolymers and their nano-assemblies. According to the composition and polymer backbone, we will summarize the self-assembly behaviors and applications of five kinds of thermo-responsive block copolymers, i.e., thermo-responsive diblock copolymers containing a hydrophilic block and a thermo-responsive block, thermo-responsive diblock copolymers containing a hydrophobic block and a thermo-responsive block, doubly thermo-responsive diblock copolymers containing two thermo-responsive blocks, thermo-responsive triblock copolymers containing a hydrophilic block and two thermoresponsive blocks, and thermo-responsive triblock copolymers containing a hydrophobic block and two thermo-responsive blocks.…”

“…Up to now, applications of thermo-responsive block copolymers have mostly focused on biomedical fields, such as imaging and drug delivery. 88–92 In this review, we mainly discuss their applications in other fields in conjunction with their properties, based on the structure of thermo-responsive block copolymers and their nano-assemblies. According to the composition and polymer backbone, we will summarize the self-assembly behaviors and applications of five kinds of thermo-responsive block copolymers, i.e.…”