Thermoresponsive polymers and their biomedical application in tissue engineering – a review

Doberenz, Falko; Zeng, Kui; Willems, Christian; Zhang, Kai; Groth, Thomas

doi:10.1039/c9tb02052g

Cited by 306 publications

(241 citation statements)

References 235 publications

Supporting

Mentioning

225

Contrasting

Order By: Relevance

“…Coatings based on thermoresponsive polymers consistently prove to be a valuable foundation for cell sheet engineering purposes [ 1 , 2 , 3 ]. Due to their physical response to changes in temperature under aqueous conditions, such coatings can switch from a rather hydrophobic, protein- and cell-adhesive state into a more hydrophilic, protein- and cell-repellant state upon cooling [ 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

Thermoresponsive Poly(glycidyl ether) Brush Coatings on Various Tissue Culture Substrates—How Block Copolymer Design and Substrate Material Govern Self-Assembly and Phase Transition

Stöbener

Weinhart

2020

Polymers

View full text Add to dashboard Cite

Thermoresponsive poly(glycidyl ether) brushes can be grafted to applied tissue culture substrates and used for the fabrication of primary human cell sheets. The self-assembly of such brushes is achieved via the directed physical adsorption and subsequent UV immobilization of block copolymers equipped with a short, photo-reactive benzophenone-based anchor block. Depending on the chemistry and hydrophobicity of the benzophenone anchor, we demonstrate that such block copolymers exhibit distinct thermoresponsive properties and aggregation behaviors in water. Independent on the block copolymer composition, we developed a versatile grafting-to process which allows the fabrication of poly(glycidyl ether) brushes on various tissue culture substrates from dilute aqueous-ethanolic solution. The viability of this process crucially depends on the chemistry and hydrophobicity of, both, benzophenone-based anchor block and substrate material. Utilizing these insights, we were able to manufacture thermoresponsive poly(glycidyl ether) brushes on moderately hydrophobic polystyrene and polycarbonate as well as on rather hydrophilic polyethylene terephthalate and tissue culture-treated polystyrene substrates. We further show that the temperature-dependent switchability of the brush coatings is not only dependent on the cloud point temperature of the block copolymers, but also markedly governed by the hydrophobicity of the surface-bound benzophenone anchor and the subjacent substrate material. Our findings demonstrate that the design of amphiphilic thermoresponsive block copolymers is crucial for their phase transition characteristics in solution and on surfaces.

show abstract

Section: Introductionmentioning

confidence: 99%

Thermoresponsive Poly(glycidyl ether) Brush Coatings on Various Tissue Culture Substrates—How Block Copolymer Design and Substrate Material Govern Self-Assembly and Phase Transition

Stöbener

Weinhart

2020

Polymers

View full text Add to dashboard Cite

show abstract

“…As one of the field's hot topics, stimuli-responsive polymers have been intensively investigated over the last 25 years. [1][2][3] In aqueous solution, these polymers typically undergo a swift, reversible change in solubility upon an external stimulus such as temperature, [4][5][6][7] pressure, [8] pH-value, [9,10] or salt concentration [11,12] (amongst others). Integrated in more complex architectures like diblock or multiblock copolymers, these stimuli-responsive polymers offer great potential for high-performance "smart" materials like switchable filtration devices [13][14][15][16][17][18] or targeted drug delivery systems.…”

Section: Introductionmentioning

confidence: 99%

Polymethacrylamide—An underrated and easily accessible upper critical solution temperature polymer: Green synthesis via photoiniferter reversible addition–fragmentation chain transfer polymerization and analysis of solution behavior in water/ethanol mixtures

Eckert

Abetz

2020

Journal of Polymer Science

View full text Add to dashboard Cite

Within the group of stimuli-responsive, "smart" materials, upper critical solution temperature (UCST) polymers remain sparsely investigated. Thus, this work focusses on a vastly ignored UCST polymer: polymethacrylamide (PMAAm). A cost-efficient photoiniferter reversible addition-fragmentation chain transfer (RAFT) polymerization yielding narrowly dispersed (Đ < 1.1) PMAAm is presented. This PMAAm exhibits highly thermoreversible UCST-type phase transitions (PT) in water/ethanol mixtures (ethanol content: 17-35 wt%) which are investigated via temperature dependent dynamic light scattering (DLS). Apart from the ethanol content, the PT temperature is affected by polymer mass fraction and chain length and varies between 10-80 C depending on the three mentioned parameters. Lastly, PMAAm's propensity towards amide hydrolysis and concomitant PT-suppression is investigated. Below temperatures of 40 C, PMAAm solutions show no sign of amide hydrolysis for at least three days, however, if heated to 70 C, the thermoresponsiveness gradually degrades within hours.

show abstract

“…To sustain life and perpetuate biological tasks, nature uses dynamic transformations of particular (macro)molecular edifices and interfaces with selective structures and functions that respond to their surroundings. Inspired by the ancestral need to mimic nature, the progress of stimuli-responsive polymers is based on the quest for similar stimuli-sensitive building blocks, architectures, and mechanisms in order to reach biological intelligence in a less intricate fashion [1][2][3]. These macromolecular constructs are able to modify their shape, volume, solubility, supramolecular arrangement, and other structural or physicochemical attributes in response to an environmental trigger [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Smart Supra- and Macro-Molecular Tools for Biomedical Applications

2020

View full text Add to dashboard Cite

Stimuli-responsive, “smart” polymeric materials used in the biomedical field function in a bio-mimicking manner by providing a non-linear response to triggers coming from a physiological microenvironment or other external source. They are built based on various chemical, physical, and biological tools that enable pH and/or temperature-stimulated changes in structural or physicochemical attributes, like shape, volume, solubility, supramolecular arrangement, and others. This review touches on some particular developments on the topic of stimuli-sensitive molecular tools for biomedical applications. Design and mechanistic details are provided concerning the smart synthetic instruments that are employed to prepare supra- and macro-molecular architectures with specific responses to external stimuli. Five major themes are approached: (i) temperature- and pH-responsive systems for controlled drug delivery; (ii) glycodynameric hydrogels for drug delivery; (iii) polymeric non-viral vectors for gene delivery; (iv) metallic nanoconjugates for biomedical applications; and, (v) smart organic tools for biomedical imaging.

show abstract

Thermoresponsive polymers and their biomedical application in tissue engineering – a review

Abstract: Thermoresponsive surfaces are used for the fabrication of cell sheets for tissue engineering purposes. Basic processes, necessary for understanding, are described and thermoresponsive polymers and their application in the biomedical field presented.

Cited by 306 publications

References 235 publications

Thermoresponsive Poly(glycidyl ether) Brush Coatings on Various Tissue Culture Substrates—How Block Copolymer Design and Substrate Material Govern Self-Assembly and Phase Transition

Thermoresponsive Poly(glycidyl ether) Brush Coatings on Various Tissue Culture Substrates—How Block Copolymer Design and Substrate Material Govern Self-Assembly and Phase Transition

Polymethacrylamide—An underrated and easily accessible upper critical solution temperature polymer: Green synthesis via photoiniferter reversible addition–fragmentation chain transfer polymerization and analysis of solution behavior in water/ethanol mixtures

Smart Supra- and Macro-Molecular Tools for Biomedical Applications

Contact Info

Product

Resources

About