Thermosensation and the TRPV channel in Rhodnius prolixus

Zermoglio, Paula; Latorre-Estivalis, José Manuel; Crespo, José E.; Lorenzo, Marcelo G.; Lazzari, Cláudio R.

doi:10.1016/j.jinsphys.2015.07.014

Cited by 34 publications

(22 citation statements)

References 74 publications

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“…Mealworms intoxicated with capsaicin or capsazepine responded similarly to mammals (in mealworms, capsaicin induced preference for lower temperatures, while capsazepine for higher temperatures). Similar findings were recently published by Zermoglio et al [ 48 ] and showed that Rhodnius prolixus treated with capsaicin is less responsive to heat stimuli, fails to orient in space, and prefers lower temperatures than non-treated insects, while capsazepine induced opposite behaviors.…”

Section: Discussionsupporting

confidence: 91%

Altered heat nociception in cockroach Periplaneta americana L. exposed to capsaicin

et al. 2018

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Some natural alkaloids, e.g. capsaicin and camphor, are known to induce a desensitization state, causing insensitivity to pain or noxious temperatures in mammals by acting on TRP receptors. Our research, for the first time, demonstrated that a phenomenon of pharmacological blockade of heat sensitivity may operate in American cockroach, Periplaneta americana (L.). We studied the escape reaction time from 50°C for American cockroaches exposed to multiple doses of different drugs affecting thermo-TRP. Capsaicin, capsazepine, and camphor induced significant changes in time spent at noxious ambient temperatures. Moreover, we showed that behavioral thermoregulation in normal temperature ranges (10–40°C) is altered in treated cockroaches, which displayed a preference for warmer regions compared to non-treated insects. We also measured the levels of malondialdehyde (MDA) and catalase activity to exclude the secondary effects of the drugs on these processes. Our results demonstrated that increase in time spent at 50°C (five versus one trial at a heat plate) induced oxidative stress, but only in control and vehicle-treated groups. In capsaicin, capsazepine, menthol, camphor and AITC-treated cockroaches the number of exposures to heat had no effect on the levels of MDA. Additionally, none of the tested compounds affected catalase activity. Our results demonstrate suppression of the heat sensitivity by repeated capsazepine, camphor and capsaicin administration in the American cockroach.

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Section: Discussionsupporting

confidence: 91%

Altered heat nociception in cockroach Periplaneta americana L. exposed to capsaicin

et al. 2018

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“…The similarity in the responses of insects and mammals indicates that capsaicin may infl uence structures that are at least functionally similar to mammalian TRPV1 (Olszewska & Tęgowska, 2011). Similar results are reported by Zermoglio et al (2015), which show that 27.6°C for females; ANOVA: F 1.10 = 0.43, P > 0.05). Based on this result, we determined the time spent by insects in the temperature gradient in areas with temperatures above 27°C in all experimental series (Fig.…”

Section: Introductionsupporting

confidence: 72%

Capsazepine affects thermal preferences of the American cockroach (Blattodea: Blattidae)

Maliszewska¹,

Tęgowska²

2016

Eur. J. Entomol.

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ISSN (online): 1802-8829http://www.eje.cz capsaicin induces preferences for lower temperatures in Rhodnius prolixus, while capsazepine treatment cause the opposite behaviour.The aim of our study was to assess the effect of capsazepine (TRPV1 antagonist) on the thermal preference of the American cockroach, Periplaneta americana. American cockroach has TRP receptors. Wicher et al. (2005) screened the Periplaneta cDNA library for members of the TRPC family and found a homolog of the Drosophila TRPɣ channel. It is proposed that it is involved in forming the channel that conducts the Ca 2+ background current in DUM neurons. Recently, French et al. (2015) demonstrated that the TRPL channel has a major role in Periplaneta phototransduction.Based on our preliminary results we hypothesized that capsazepine infl uences thermoregulatory processes in insects. To confi rm this hypothesis, we examined thermal preferences of cockroaches treated with fi ve different capsazepine concentrations and released in a thermal gradient system. To establish whether capsazepine may act as a capsaicin antagonist in insects, we assessed the insect thermoregulatory response to capsaicin following pretreatment with capsazepine. We assumed that capsazepine blocks the insects' hypothermic response to capsaicin, as in mammals (Dogan et al., 2004). MATERIALS AND METHODS InsectsAmerican cockroaches Periplaneta americana L. were reared in plastic containers at ~ 26°C under a natural photoperiod. They were fed with oat fl akes and apples and received drinking water at regular intervals. Only adult individuals were selected for the experiments. Experiments were performed separately on males Abstract. Capsazepine is a competitive antagonist of capsaicin, a TRPV1 agonist responsible for the spicy taste of pepper. TRPV1 agonists and antagonists are known to affect mammalian body temperature, but their action on thermoregulation in insects is poorly known. In this study we evaluated the effect of capsazepine on the thermal preference of the American cockroach, Periplaneta americana using a thermal gradient. Our results revealed that capsazepine in submicromolar concentrations induces a preference for higher ambient temperatures when compared to the control insects. To assess whether capsazepine may act also as an antagonist of capsaicin in insects, we determined this insects' thermal behaviour when capsazepine was applied before capsaicin. The hypothermic response to capsaicin was clearly blocked by pre-treatment with capsazepine only in female American cockroaches. Our results indicate the involvement of structures functionally similar to TRPV1 in insect thermosensation.

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“…A recent study suggested that a TRP belonging to vanilloid receptors family (TRPVs) could be involved in the high sensitivity to heat in R . prolixus which uses these capacities to perceive warm-blooded hosts [97]. In T .…”

Section: Discussionmentioning

confidence: 99%

Under-Expression of Chemosensory Genes in Domiciliary Bugs of the Chagas Disease Vector Triatoma brasiliensis

et al. 2016

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BackgroundIn Latin America, the bloodsucking bugs Triatominae are vectors of Trypanosoma cruzi, the parasite that causes Chagas disease. Chemical elimination programs have been launched to control Chagas disease vectors. However, the disease persists because native vectors from sylvatic habitats are able to (re)colonize houses—a process called domiciliation. Triatoma brasiliensis is one example. Because the chemosensory system allows insects to interact with their environment and plays a key role in insect adaption, we conducted a descriptive and comparative study of the chemosensory transcriptome of T. brasiliensis samples from different ecotopes.Methodology/Principal FindingIn a reference transcriptome built using de novo assembly, we found transcripts encoding 27 odorant-binding proteins (OBPs), 17 chemosensory proteins (CSPs), 3 odorant receptors (ORs), 5 transient receptor potential channel (TRPs), 1 sensory neuron membrane protein (SNMPs), 25 takeout proteins, 72 cytochrome P450s, 5 gluthatione S-transferases, and 49 cuticular proteins. Using protein phylogenies, we showed that most of the OBPs and CSPs for T. brasiliensis had well supported orthologs in the kissing bug Rhodnius prolixus. We also showed a higher number of these genes within the bloodsucking bugs and more generally within all Hemipterans compared to the other species in the super-order Paraneoptera. Using both DESeq2 and EdgeR software, we performed differential expression analyses between samples of T. brasiliensis, taking into account their environment (sylvatic, peridomiciliary and domiciliary) and sex. We also searched clusters of co-expressed contigs using HTSCluster. Among differentially expressed (DE) contigs, most were under-expressed in the chemosensory organs of the domiciliary bugs compared to the other samples and in females compared to males. We clearly identified DE genes that play a role in the chemosensory system.Conclusion/SignificanceChemosensory genes could be good candidates for genes that contribute to adaptation or plastic rearrangement to an anthropogenic system. The domiciliary environment probably includes less diversity of xenobiotics and probably has more stable abiotic parameters than do sylvatic and peridomiciliary environments. This could explain why both detoxification and cuticle protein genes are less expressed in domiciliary bugs. Understanding the molecular basis for how vectors adapt to human dwellings may reveal new tools to control disease vectors; for example, by disrupting chemical communication.

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Thermosensation and the TRPV channel in Rhodnius prolixus

Cited by 34 publications

References 74 publications

Altered heat nociception in cockroach Periplaneta americana L. exposed to capsaicin

Altered heat nociception in cockroach Periplaneta americana L. exposed to capsaicin

Capsazepine affects thermal preferences of the American cockroach (Blattodea: Blattidae)

Under-Expression of Chemosensory Genes in Domiciliary Bugs of the Chagas Disease Vector Triatoma brasiliensis

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