Thermosensitive alginate–gelatin–nitrogen-doped carbon dots scaffolds as potential injectable hydrogels for cartilage tissue engineering applications

Ghanbari, Mojgan; Salavati‐Niasari, Masoud; Mohandes, Fatemeh

doi:10.1039/d1ra01496j

Cited by 63 publications

(24 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, a relative reduction in the intensity of the symmetrical COC stretching (≈810 cm −1 , cyclic ether) was observed in OA samples, confirming the chain cleavage. [ 12,14a,18 ] Furthermore, we identified a new band at ≈1720 cm −1 , corresponding to the CO stretching mode of aldehyde groups, demonstrating that the oxidation process was successful (Figure 2a). [ 18 ]…”

Section: Resultsmentioning

confidence: 99%

“…Several studies have focused on modifying CNTs’ physicochemical properties and enhancing their compatibility with mammalian cells by mixing CNTs with polymers such as poly‐ dl ‐lactide, [ 8 ] collagen, [7a,9] gelatin, [ 10 ] or hydroxyapatite. [ 11 ] CN x show more positive effects on cellular response than other nanotubes (i.e., single‐walled, double‐walled, multiwalled, nonfunctionalized, or functionalized); which could be due to the presence of nitrogen in the graphitic network [5b,5c,12] …”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Highly Concentrated Nitrogen‐Doped Carbon Nanotubes in Alginate–Gelatin 3D Hydrogels Enable in Vitro Breast Cancer Spheroid Formation

Munguia-Lopez

Jiang

Ferlatte

et al. 2021

Advanced NanoBiomed Research

View full text Add to dashboard Cite

Carbon nanotubes’ (CNTs) physicochemical and mechanical properties make them ideal reinforcement materials for hydrogels, but distributing CNTs homogeneously in hydrogels remains a challenge. Chemical modifications to CNTs are used to facilitate nanomaterial dispersion, thus improving hydrogels’ physicochemical properties. Among CNTs, nitrogen‐doped CNTs (CNx) possess both great dispersibility in solution and biocompatibility properties. By formulating a method to incorporate CNx within alginate (i.e., covalently grafting alginate to the CNx surface versus noncovalently adsorbing alginate to the CNx surface) creates extrudable materials with tunable physical, chemical, and thermal properties. Herein, three new composites of alginate‐CNx are created. The results indicate that all composites present different physicochemical and thermal properties, suggesting that alginate is reorganized according to their degree of oxidation. These composites show cytocompatibility with MDA‐MB‐231 and regulation over the size of spheroids formed within the matrix. CNx within the matrix negatively affects MCF‐7 cells viability, spheroid formation rate, and the quantity of spheroids developed during culture. These materials provide a useful 3D hydrogel that can be used to develop in vitro models to understand the role of microenvironmental factors such as stiffness or surface roughness on the development of spheroids and their subsequent phenotypic behavior.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Highly Concentrated Nitrogen‐Doped Carbon Nanotubes in Alginate–Gelatin 3D Hydrogels Enable in Vitro Breast Cancer Spheroid Formation

Munguia-Lopez

Jiang

Ferlatte

et al. 2021

Advanced NanoBiomed Research

View full text Add to dashboard Cite

show abstract

“…To overcome these issues, conjugated hydrogels have been developed to achieve enhanced mechanical properties and stability in physiological environments [ 23 ]. The mechanical strength and stability of conjugated hydrogels is commonly improved with the aid of chemical crosslinkers (i.e., glutaraldehyde [ 32 ], glucose [ 33 ], genipin [ 23 ], 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), and N-hydroxysuccinimide (NHS) [ 34 ]) through forming covalent bonds between polymer chains.…”

Section: Introductionmentioning

confidence: 99%

An Electroconductive, Thermosensitive, and Injectable Chitosan/Pluronic/Gold-Decorated Cellulose Nanofiber Hydrogel as an Efficient Carrier for Regeneration of Cardiac Tissue

et al. 2022

Self Cite

View full text Add to dashboard Cite

Myocardial infarction is a major cause of death worldwide and remains a social and healthcare burden. Injectable hydrogels with the ability to locally deliver drugs or cells to the damaged area can revolutionize the treatment of heart diseases. Herein, we formulate a thermo-responsive and injectable hydrogel based on conjugated chitosan/poloxamers for cardiac repair. To tailor the mechanical properties and electrical signal transmission, gold nanoparticles (AuNPs) with an average diameter of 50 nm were physically bonded to oxidized bacterial nanocellulose fibers (OBC) and added to the thermosensitive hydrogel at the ratio of 1% w/v. The prepared hydrogels have a porous structure with open pore channels in the range of 50–200 µm. Shear rate sweep measurements demonstrate a reversible phase transition from sol to gel with increasing temperature and a gelation time of 5 min. The hydrogels show a shear-thinning behavior with a shear modulus ranging from 1 to 12 kPa dependent on gold concentration. Electrical conductivity studies reveal that the conductance of the polymer matrix is 6 × 10−2 S/m at 75 mM Au. In vitro cytocompatibility assays by H9C2 cells show high biocompatibility (cell viability of >90% after 72 h incubation) with good cell adhesion. In conclusion, the developed nanocomposite hydrogel has great potential for use as an injectable biomaterial for cardiac tissue regeneration.

show abstract

“…Hydrogels are a type of three-dimensional polymer network that have a large number of pore structures; hence, the exchange and circulation of substances becomes easy [ 1 , 2 ]. Natural polymer hydrogels with good biocompatibility and biodegradation can support cell adhesion, proliferation, and differentiation on its surface or inside.…”

Section: Introductionmentioning

confidence: 99%

Preparation and Properties of Double-Crosslinked Hydroxyapatite Composite Hydrogels

Zhao

et al. 2022

IJMS

View full text Add to dashboard Cite

Natural polymer hydrogels have good mechanical properties and biocompatibility. This study designed hydroxyapatite-enhanced photo-oxidized double-crosslinked hydrogels. Hyaluronic acid (HA) and gelatin (Gel) were modified with methacrylate anhydride. The catechin group was further introduced into the HA chain inspired by the adhesion chemistry of marine mussels. Hence, the double-crosslinked hydrogel (HG) was formed by the photo-crosslinking of double bonds and the oxidative-crosslinking of catechins. Moreover, hydroxyapatite was introduced into HG to form hydroxyapatite-enhanced hydrogels (HGH). The results indicate that, with an increase in crosslinking network density, the stiffness of hydrogels became higher; these hydrogels have more of a compact pore structure, their anti-degradation property is improved, and swelling property is reduced. The introduction of hydroxyapatite greatly improved the mechanical properties of hydrogels, but there is no change in the stability and crosslinking network structure of hydrogels. These inorganic phase-enhanced hydrogels were expected to be applied to tissue engineering scaffolds.

show abstract

Thermosensitive alginate–gelatin–nitrogen-doped carbon dots scaffolds as potential injectable hydrogels for cartilage tissue engineering applications

Abstract: The low gelation time (120 s) and gelation temperature at body temperature (37 °C) make oxidized alginate/gelatin/NCDs hydrogels suitable as temperature-sensitive injectable hydrogels for cartilage tissue engineering.

Cited by 63 publications

References 40 publications

Highly Concentrated Nitrogen‐Doped Carbon Nanotubes in Alginate–Gelatin 3D Hydrogels Enable in Vitro Breast Cancer Spheroid Formation

Highly Concentrated Nitrogen‐Doped Carbon Nanotubes in Alginate–Gelatin 3D Hydrogels Enable in Vitro Breast Cancer Spheroid Formation

An Electroconductive, Thermosensitive, and Injectable Chitosan/Pluronic/Gold-Decorated Cellulose Nanofiber Hydrogel as an Efficient Carrier for Regeneration of Cardiac Tissue

Preparation and Properties of Double-Crosslinked Hydroxyapatite Composite Hydrogels

Contact Info

Product

Resources

About