Thermosensitive hybrid hydrogels for the controlled release of bioactive vancomycin in the treatment of orthopaedic implant infections

Censi, Roberta; Casadidio, Cristina; Dubbini, Alessandra; Cortese, Manuela; Scuri, Stefania; Grappasonni, Iolanda; Golob, Samuel; Vojnović, Đ.; Sabbieti, Maria Giovanna; Agas, Dimitrios; Martino, Piera Di

doi:10.1016/j.ejpb.2019.07.006

Cited by 24 publications

(15 citation statements)

References 59 publications

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“…The proposed hydrogel already showed promising results in the field of protein release and tissue regeneration [6][7][8][9].…”

Section: Discussionmentioning

confidence: 98%

“…In our previous work, we developed a new rapidly in situ gelling and biodegradable hydrogels based on hyaluronic acid and poly(hydroxypropyl methacrylamide lactate)-polyethylene glycol (p(HPMAm-lac)-PEG) triblock copolymers [5] and assessed its cytocompatibility in vitro with mesenchymal stem cells and fibroblasts, as well as its biocompatibility in vivo [6]. The developed hydrogels were demonstrated to possess the capability to controllably release and protect from degradation a number of model and therapeutic proteins, [7,8] as well as to encapsulate viable cells for cartilage tissue engineering applications. [9] In the present study, a novel interpenetrating hydrogel network composed of two interlaced polymeric networks independently cross-linked via covalent or ionic bonds was designed and synthesized by combining the previously developed thermosensitive hybrid hydrogel with activated platelet-rich plasma (PRP) from equine source.…”

Section: Introductionmentioning

confidence: 99%

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Interpenetrating Hydrogel Networks Enhance Mechanical Stability, Rheological Properties, Release Behavior and Adhesiveness of Platelet-Rich Plasma

Censi

Casadidio

Deng

et al. 2020

IJMS

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Platelet-rich plasma (PRP) has attracted much attention for the treatment of articular cartilage defects or wounds due to its intrinsic content of growth factors relevant for tissue repair. However, the short residence time of PRP in vivo, due to the action of lytic enzymes, its weak mechanical properties and the consequent short-term release of bioactive factors has restricted its application and efficacy. The present work aimed at designing new formulation strategies for PRP, based on the use of platelet concentrate (PC)-loaded hydrogels or interpenetrating polymer networks, directed at improving mechanical stability and sustaining the release of bioactive growth factors over a prolonged time-span. The interpenetrating hydrogels comprised two polymer networks interlaced on a molecular scale: (a) a first covalent network of thermosensitive and biodegradable vinyl sulfone bearing p(hydroxypropyl methacrylamide-lacate)-polyethylene glycol triblock copolymers, tandem cross-linked by thermal gelation and Michael addition when combined with thiolated hyaluronic acid, and (b) a second network composed of cross-linked fibrin. The PC-loaded hydrogels, instead, was formed only by network (a). All the designed and successfully synthesized formulations greatly increased the stability of PRP in vitro, leading to significant increase in degradation time and storage modulus of PRP gel. The resulting viscoelastic networks showed the ability to controllably release platelet derived growth factor and transforming growth factr β1, and to improve the tissue adhesiveness of PRP. The newly developed hydrogels show great potential for application in the field of wound healing, cartilage repair and beyond.

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“…The proposed hydrogel already showed promising results in the field of protein release and tissue regeneration [6][7][8][9].…”

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Interpenetrating Hydrogel Networks Enhance Mechanical Stability, Rheological Properties, Release Behavior and Adhesiveness of Platelet-Rich Plasma

Censi

Casadidio

Deng

et al. 2020

IJMS

Self Cite

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“…PLS-based in situ-forming hydrogels are able to improve the efficacy of the loaded drug(s) while overcoming different drawbacks like: Systemic drug toxicity, high peak plasma concentrations and rapid drug degradation. In this work we investigated acidic PLS hydrogels as matrices for the stabilization and the release of vancomycin (VAN) [26,27].…”

Section: Nanoemulsions As Delivery Systems For Poly-chemotherapy Aimimentioning

confidence: 99%

Steering the Clinical Translation of Delivery Systems for Drugs and Health Products

Pignatello

Matricardi

2020

Pharmaceutics

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Besides the feasibility for industrial scale-up, accelerating the translation from bench to bedside of new technological strategies for controlled delivery and targeting of drugs and other actives relevant for health management, such as medical devices and nutraceuticals, would benefit from an even earlier evaluation in pre-clinical models and clinical settings. At the same time, translational medicine also performs in the opposite direction, incorporating clinical needs and observations into scientific hypotheses and innovative technological proposals. With these aims, the sessions proposed for the 2019 CRS Italy Chapter Workshop will introduce the experience of Italian and worldwide researchers on how to foster the actual work in controlled release and drug delivery towards a reliable pre-clinical and clinical assessment.

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“…The entrapment method is based on the retention of biomolecules within a hydrogel network that allows solvent molecules to pass through its porous structure but retains the biomolecules of interest. In general, the diffusion-dominated release of biomolecules cannot achieve over extended time points beyond a few hours to a day [ 197 ]. When the pore size approaches the biomolecule size, the effect of steric hindrance on biomolecules diffusion becomes dominant and the release rate slows down.…”

Section: Introduction Of Biomolecules Within the Hydrogel Networkmentioning

confidence: 99%

Design of Bio-Conjugated Hydrogels for Regenerative Medicine Applications: From Polymer Scaffold to Biomolecule Choice

et al. 2020

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Bio-conjugated hydrogels merge the functionality of a synthetic network with the activity of a biomolecule, becoming thus an interesting class of materials for a variety of biomedical applications. This combination allows the fine tuning of their functionality and activity, whilst retaining biocompatibility, responsivity and displaying tunable chemical and mechanical properties. A complex scenario of molecular factors and conditions have to be taken into account to ensure the correct functionality of the bio-hydrogel as a scaffold or a delivery system, including the polymer backbone and biomolecule choice, polymerization conditions, architecture and biocompatibility. In this review, we present these key factors and conditions that have to match together to ensure the correct functionality of the bio-conjugated hydrogel. We then present recent examples of bio-conjugated hydrogel systems paving the way for regenerative medicine applications.

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Thermosensitive hybrid hydrogels for the controlled release of bioactive vancomycin in the treatment of orthopaedic implant infections

Cited by 24 publications

References 59 publications

Interpenetrating Hydrogel Networks Enhance Mechanical Stability, Rheological Properties, Release Behavior and Adhesiveness of Platelet-Rich Plasma

Interpenetrating Hydrogel Networks Enhance Mechanical Stability, Rheological Properties, Release Behavior and Adhesiveness of Platelet-Rich Plasma

Steering the Clinical Translation of Delivery Systems for Drugs and Health Products

Design of Bio-Conjugated Hydrogels for Regenerative Medicine Applications: From Polymer Scaffold to Biomolecule Choice

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