Thermosensitive hydrogels for local delivery of 5-fluorouracil as neoadjuvant or adjuvant therapy in colorectal cancer

Sabbagh, Chantal Al; Séguin, Johanne; Агапова, Е. Д.; Kramerich, Dounia; Boudy, Vincent; Mignet, Nathalie

doi:10.1016/j.ejpb.2020.10.011

Cited by 44 publications

(16 citation statements)

References 54 publications

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“… 56 − 58 Such thermo-responsive hydrogels are advantageous because they are simple to prepare and inject. 59 , 60 Chitosan/GP hydrogels have been extensively used for drug delivery, 61 , 62 tissue regeneration/repair, 63 , 64 and the slow release of nanoparticles. 65 , 66 …”

Section: Introductionmentioning

confidence: 99%

Injectable Slow-Release Hydrogel Formulation of a Plant Virus-Based COVID-19 Vaccine Candidate

et al. 2022

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Cowpea mosaic virus (CPMV) is a potent immunogenic adjuvant and epitope display platform for the development of vaccines against cancers and infectious diseases, including coronavirus disease 2019. However, the proteinaceous CPMV nanoparticles are rapidly degraded in vivo. Multiple doses are therefore required to ensure long-lasting immunity, which is not ideal for global mass vaccination campaigns. Therefore, we formulated CPMV nanoparticles in injectable hydrogels to achieve slow particle release and prolonged immunostimulation. Liquid formulations were prepared from chitosan and glycerophosphate (GP) before homogenization with CPMV particles at room temperature. The formulations containing high-molecular-weight chitosan and 0–4.5 mg mL –1 CPMV gelled rapidly at 37 °C (5–8 min) and slowly released cyanine 5-CPMV particles in vitro and in vivo. Importantly, when a hydrogel containing CPMV displaying severe acute respiratory syndrome coronavirus 2 spike protein epitope 826 (amino acid 809–826) was administered to mice as a single subcutaneous injection, it elicited an antibody response that was sustained over 20 weeks, with an associated shift from Th1 to Th2 bias. Antibody titers were improved at later time points (weeks 16 and 20) comparing the hydrogel versus soluble vaccine candidates; furthermore, the soluble vaccine candidates retained Th1 bias. We conclude that CPMV nanoparticles can be formulated effectively in chitosan/GP hydrogels and are released as intact particles for several months with conserved immunotherapeutic efficacy. The injectable hydrogel containing epitope-labeled CPMV offers a promising single-dose vaccine platform for the prevention of future pandemics as well as a strategy to develop long-lasting plant virus-based nanomedicines.

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Section: Introductionmentioning

confidence: 99%

Injectable Slow-Release Hydrogel Formulation of a Plant Virus-Based COVID-19 Vaccine Candidate

et al. 2022

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“…Some interesting research work has been developed on thermosensitive poloxamer-based hydrogels, particularly with poloxamer 188 (P188) and 407 (P407). Indeed, thermosensitive hydrogels for buccal drug delivery have been designed and described in scientific literature [29][30][31][32][33]. These kind of hydrogels can undergo a sol-gel transition depending on the temperature [34,35].…”

Section: Introductionmentioning

confidence: 99%

Mucoadhesive Poloxamer-Based Hydrogels for the Release of HP-β-CD-Complexed Dexamethasone in the Treatment of Buccal Diseases

Diaz-Salmeron¹,

Toussaint

Huang

et al. 2021

Pharmaceutics

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Oral lichen planus (OLP) is an ongoing and chronic inflammatory disease affecting the mucous membrane of the oral cavity. Currently, the treatment of choice consists in the direct application into the buccal cavity of semisolid formulations containing a corticosteroid molecule to decrease inflammatory signs and symptoms. However, this administration route has shown various disadvantages limiting its clinical use and efficacy. Indeed, the frequency of application and the incorrect use of the preparation may lead to a poor efficacy and limit the treatment compliance. Furthermore, the saliva clearance and the mechanical stress present in the buccal cavity also involve a decrease in the mucosal exposure to the drug. In this context, the design of a new pharmaceutical formulation, containing a steroidal anti-inflammatory, mucoadhesive, sprayable and exhibiting a sustained and controlled release seems to be suitable to overcome the main limitations of the existing pharmaceutical dosage forms. The present work reports the formulation, optimization and evaluation of the mucoadhesive and release properties of a poloxamer 407 thermosensitive hydrogel containing a poorly water-soluble corticosteroid, dexamethasone acetate (DMA), threaded into hydroxypropyl-beta-cyclodextrin (HP-β-CD) molecules. Firstly, physicochemical properties were assessed to ensure suitable complexation of DMA into HP-β-CD cavities. Then, rheological properties, in the presence and absence of various mucoadhesive agents, were determined and optimized. The hydration ratio (0.218–0.191), the poloxamer 407 (15–17 wt%) percentage and liquid-cyclodextrin state were optimized as a function of the gelation transition temperature, viscoelastic behavior and dynamic flow viscosity. Deformation and resistance properties were evaluated in the presence of various mucoadhesive compounds, being the sodium alginate and xanthan gum the most suitable to improve adhesion and mucoadhesion properties. Xanthan gum was shown as the best agent prolonging the hydrogel retention time up to 45 min. Furthermore, xanthan gum has been found as a relevant polymer matrix controlling drug release by diffusion and swelling processes in order to achieve therapeutic concentration for prolonged periods of time.

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“…Hence, these supramolecular architectures may constitute the basis for novel smart nanomaterials with improved properties such as in vitro and in vivo physicochemical stability (Park et al, 2007), efficiency via drug loading improvement (Ahmed et al, 2019), exogenous environment adaptability (Pedersen et al, 2020), higher safety (Martin et al, 2020), manufacturability (Wren et al, 2020), and may have a broad range of applications with various interfaces, i.e., liquid/liquid (Prevot et al, 2018), solid/liquid (Couillaud et al, 2019), and gas/liquid (Manta et al, 2016;Corvis et al, 2018). Indeed, self-assembled systems (Beingessner et al, 2016;Mohamed et al, 2019) have been developed and widely explored in drug delivery (Chen et al, 2011;Song et al, 2011;Desmaële et al, 2012;Mignet et al, 2012;Al Sabbagh et al, 2020), gene delivery (Manta et al, 2017;Do et al, 2019), biomedical engineering (Sun et al, 2012;Childs et al, 2013;Meng et al, 2013;Puzan et al, 2018;Zhou et al, 2020), medicine (Journeay et al, 2008(Journeay et al, , 2009Sun et al, 2014), and diagnostics. This body of work has led to the emergence of the field of supramolecular nanomedicine, which is the focus of this Research Topic for Frontiers in Chemistry (Figure 1).…”

Section: Editorial On the Research Topic Supramolecular Nanomaterialsmentioning

confidence: 99%

Editorial: Supramolecular Nanomaterials for Engineering, Drug Delivery, and Medical Applications

et al. 2020

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Thermosensitive hydrogels for local delivery of 5-fluorouracil as neoadjuvant or adjuvant therapy in colorectal cancer

Cited by 44 publications

References 54 publications

Injectable Slow-Release Hydrogel Formulation of a Plant Virus-Based COVID-19 Vaccine Candidate

Injectable Slow-Release Hydrogel Formulation of a Plant Virus-Based COVID-19 Vaccine Candidate

Mucoadhesive Poloxamer-Based Hydrogels for the Release of HP-β-CD-Complexed Dexamethasone in the Treatment of Buccal Diseases

Editorial: Supramolecular Nanomaterials for Engineering, Drug Delivery, and Medical Applications

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