Thermosensitive polymers as carriers for DNA delivery

Hinrichs, Wouter L. J.; Schuurmans-Nieuwenbroek, N.M.E.; Wetering, Petra van de; Hennink, Wim E.

doi:10.1016/s0168-3659(99)00075-9

Cited by 175 publications

(116 citation statements)

References 26 publications

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“…17, 18 Hinrichs and colleagues prepared linear copolymers of P[NIPAM/DMAEMA] and evaluated the effect of chain length and copolymer composition on binding of pDNA and transfection efficiency of OVCAR-3 cells (human ovarian cancer). 19 The authors observed a decrease in transfection efficiency of polyplexes with increasing content of NIPAM in the copolymers, though their results did not suggest a cooperative role of P [NIPAM] in the release of pDNA.…”

Section: Dovepressmentioning

confidence: 91%

Characterization of complexation of poly (N-isopropylacrylamide-co-2-(dimethylamino) ethyl methacrylate) thermoresponsive cationic nanogels with salmon sperm DNA

Moselhy

Vira²,

Liu³

et al. 2009

IJN

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Thermoresponsive cationic nanogel (TCNG) networks based on N-isopropylacrylamide (NIPAM), 2-(dimethylamino)ethyl methacrylate (DMAEMA), and quaternary alkyl ammonium halide salts of DMAEMA (DMAEMAQ) were synthesized by dispersion polymerization technique. The thermoresponsive properties of TCNGs and TCNG-salmon sperm DNA (sasDNA) polyplexes were characterized in aqueous media of various pH and ionic strength. The distinct phase-transition properties of P[NIPAM/DMAEMA]/sasDNA and P[NIPAM/ DMAEMAQ]/sasDNA polyplexes were attributed to the condensing capability of polycations and to differences in the spatial distribution of structural charges in quaternized and nonquaternized networks. The findings demonstrate that stable TCNGs can be prepared with controllable responsive properties determined by the nature of the cationic charge incorporated and may have potential as vehicles for DNA delivery.

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Section: Dovepressmentioning

confidence: 91%

Characterization of complexation of poly (N-isopropylacrylamide-co-2-(dimethylamino) ethyl methacrylate) thermoresponsive cationic nanogels with salmon sperm DNA

Moselhy

Vira²,

Liu³

et al. 2009

IJN

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show abstract

“…Hinrichs et al 16 have synthesized thermo-responsive copolymers of NIPA (for temperature sensitivity) and DMAEMA (for positive charge/amino groups) and investigated the transfection efficiency in ovarian cancer cells. They prepared a series of copolymers with various monomer ratios and molecular weights by free radical polymerization in organic or aqueous media.…”

Section: Intelligent Polymeric Vectors In Gene Therapymentioning

confidence: 99%

Intelligent polymers as nonviral vectors

2005

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The successful gene therapy largely depends on the vector type that allows a selective and efficient gene delivery to target cells with minimal toxicity. Nonviral vectors are much safer and cheaper, can be produced easily in large quantities, and have higher genetic material carrying capacity. However, they are generally less efficient in delivering DNA and initiating gene expression as compared to viral vectors, particularly when used in vivo. As nonviral vectors, polycations may work well for efficient cell uptake and endosomal escape, because they do form compact and smaller complexes with plasmid DNA and carry amine groups, which give positive charge and buffering ability that allows safe escape from endosome/lysosome. However, this is a disadvantage in the following step, which is releasing the plasmid DNA within the cytosol. In order to initiate transcription and enhance gene expression, the polymer/plasmid complex should dissociate after releasing from endosome safely and effectively. There are also other limitations with some of the polycationic carriers, for example, aggregation, toxicity, etc. Intelligent polymers, also called as 'stimuli responsive polymers', have a great potential as nonviral vectors to obtain site-, timing-, and duration period-specific gene expression, which is already exhibited in recent studies that are briefly summarized here. Gene Therapy (2005) 12, S139-S145.

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“…Polymers whose LCST value decreases with increasing molecular weight are known as type I, whereas polymers of type II have LCST positions that are insensitive to molecular weight. [24][25][26][27][28][29][30][31][32] For some water soluble polymers, such as PEG, 24 poly(2-methyl-2-oxazoline) (pMeOx), and poly(N-(2-hydroxypropyl) methacrylamide (pHPMA), the LCST value is above 100 ºC, making them soluble at temperatures below water's boiling point. Polymers whose phase behaviour changes in solution as a response to variations in the environmental temperature are called thermosensitive polymers.…”

Section: Introductionmentioning

confidence: 99%

Novel thermosensitive telechelic PEGs with antioxidant activity: synthesis, molecular properties and conformational behaviour

et al. 2014

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We report on the synthesis and solution properties of novel tailor -made polymer conjugates, which are highly compelling for biomedical applications due to their antioxidant activity and the potential to fine-tune their thermosensitive properties. These conjugates consist of polyethylene glycol (PEG polymers containing antioxidant moieties, namely 3 -(3,5-di-tertbutyl-4-hydroxyphenyl)propionate or 2-benzamido-3-(3,5-di-tert-butyl-4-hydroxyphenyl)acrylate, as end groups that differ in activity and hydrophobicity. It was shown that all of the synthesised conjugates have low critical solution temperatures (LCSTs) characteristic of type II polymers on a phase diagram. By simply varying the PEG molecular weight, the solution properties, including the LCST value, could be easily tuned across a broad temperature range of 20-90 ºC, providing an ideal method for the creation of thermosensitive polymers. It was also established that the LCST value and the polymer conjugate conformation depend on the antioxidant structure. From dynamic light scattering and small-angle X-ray scattering data, we were able to construct a complete sequence diagram of the conformational phase behaviour for the polymers with increasing temperature. It was observed that the conjugate conformation changes are the result of water shifting from a thermodynamically favourable solvent to an unfavourable one. This process then leads to compaction of the conjugate, followed by its aggregation.

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Thermosensitive polymers as carriers for DNA delivery

Cited by 175 publications

References 26 publications

Characterization of complexation of poly (N-isopropylacrylamide-co-2-(dimethylamino) ethyl methacrylate) thermoresponsive cationic nanogels with salmon sperm DNA

Characterization of complexation of poly (N-isopropylacrylamide-co-2-(dimethylamino) ethyl methacrylate) thermoresponsive cationic nanogels with salmon sperm DNA

Intelligent polymers as nonviral vectors

Novel thermosensitive telechelic PEGs with antioxidant activity: synthesis, molecular properties and conformational behaviour

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