Thermosensitive receptors in neural stem cells link stress-induced hyperthermia to impaired neurogenesis via microglial engulfment

Hoshi, Yutaka; Shibasaki, Kazuo; Gailly, Philippe; Ikegaya, Yuji; Koyama, Ryuta

doi:10.1126/sciadv.abj8080

Cited by 16 publications

(8 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The neural progenitor cells are majorly found in the subventricular zone and hippocampus, and they are also found in other brain regions including hypothalamus, substantia nigra, and striatum (Jurkowski et al, 2020). A recent study reported impaired neurogenesis in the mice exposed to social defeat stress-induced hyperthermia (Hoshi et al, 2021). Previously, neural progenitor cells were reported to migrate from subventricular zone to corpus callosum upon damage (Mallett et al, 2018;Nait-Oumesmar et al, 1999) and under normal conditions (Sohn et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Hyperthermia alters neurobehavior by affecting cell proliferation and neuronal survival in young male rats

Mete

Eyuboglu

Vitrinel

et al. 2023

Intl J of Devlp Neuroscience

View full text Add to dashboard Cite

Maintenance of body temperature within physiological range is critical for the fetal and neonatal development. Hyperthermia is one of the most frequently encountered pediatric complaints and may cause neurological disorders due to neuronal injury. In this study, we aimed to investigate the effects of hyperthermia on behavioral alterations, neuronal survival, apoptosis, and cell proliferation in young male Sprague–Dawley rats. Twenty‐one 13‐day‐old rats were randomly divided into three groups (n = 7 per group). Body temperature was increased to 39°C and 41°C in a hyperthermia induction chamber for 30 min, whereas the animals in control group were maintained at 36°C. Twenty‐four hours after hyperthermia, animals were subjected to the open field test, elevated‐O‐maze test, and grip strength test to assess the locomotor activity, anxiety, and motor function. Neuronal survival, apoptosis, and cell proliferation were investigated in cortex, hippocampal dentate gyrus (DG) and CA1 regions, and corpus callosum (CC). Decreased locomotor activity and motor function and increased anxiety were observed in the hyperthermia groups, and these were more pronounced in the 41°C group. Neuronal survival was significantly decreased in DG, CA1, and CC in the hyperthermia groups (**p < 0.01). Apoptosis was significantly induced in cortex, DG, and CC of the animals exposed to heat (*p < 0.05). In addition, cell proliferation positivity decreased significantly only in DG and CC of the animals exposed to heat (*p < 0.05). Our results suggest that neurobehavioral deficits caused by hyperthermia may be due to the increased apoptosis and neuronal cell death and decreased cell proliferation in the brain of postnatal developing rats.

show abstract

Section: Discussionmentioning

confidence: 99%

Hyperthermia alters neurobehavior by affecting cell proliferation and neuronal survival in young male rats

Mete

Eyuboglu

Vitrinel

et al. 2023

Intl J of Devlp Neuroscience

View full text Add to dashboard Cite

show abstract

“…Mechanistically, miR-146a-5p decreased the expression of important regulators of neurogenesis, including krüppel-like factor 4 and cyclin-dependent kinase-like 5, reducing neurogenesis and inducing depressive-like behavior thereafter [58]. Another recent study highlighted how microglial phagocytosis alters neurogenesis in the CSDS model [59]. Phosphatidylserine (PS)-expressing NSCs were found in the DG; NSCs express thermosensitive transient receptor potential vanilloid 4 (TRPV4), which was activated by CSDS.…”

Section: Role Of Microglial Inflammatory Signaling In the Pathology O...mentioning

confidence: 99%

Microglial Responses to Stress-Induced Depression: Causes and Consequences

Suk¹

2023

Preprint

View full text Add to dashboard Cite

Chronic stress is a major risk factor for various psychiatric diseases, including depression; it induces a range of cellular and structural adaptations, culminating in altered neurocircuitry and subsequent depression. Accumulating evidence suggests that microglial cells orchestrate stress-induced depression. Preclinical studies of stress-induced depression revealed microglial inflammatory activation in regions of the brain that regulate mood. Although studies have identified several molecules that trigger inflammatory responses in microglia, the pathways that regulate stress-induced microglial activation remain unclear. Understanding the exact triggers that induce microglial inflammatory activation can help find therapeutic targets to treat depression. In the current review, we summarize the recent literature on possible sources of microglial inflammatory activation in animal models of chronic stress-induced depression. In addition, we describe how microglial inflammatory signaling affects neuronal health and causes depressive behavior. Finally, we propose ways to target the microglial inflammatory cascade to treat depressive disorders.

show abstract

“…The hippocampal sections were incubated in PBS, 0.3% Triton X-100, and 10% goat serum at room temperature for 30 min and washed with PBS. The tissues were subjected to the Click-iT reaction (Thermo Fisher Scienti c) to uorescently visualize EdU-labeled cells with Alexa Fluor 594 for detection 42 and washed with PBS, followed by counterstaining with NeuroTrace 435/455 blue uorescent Nissl stain (1:500, N21479, Thermo Fisher Scienti c) in PBS (containing Triton X-100) for 1.5 h at room temperature.…”

Section: Edu Labelingmentioning

confidence: 99%

Rodent control of chamber brightness leads to the emergence of unique circadian rhythms

Ogasawara

Takeuchi

Matsumoto

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

Internal circadian rhythms deviate slightly from 24 h, depending on species and individuals; thus, an environment with a 24-h light/dark cycle may not be ideal. In this study, we enabled mice to control room brightness via nose pokes and investigated subsequent biological rhythms as well as physiological and cognitive functions. The mice immediately learned the relationship between nose pokes and the resulting illuminance change and generated 24.5 ± 0.4 h-cycles in both illuminance and locomotor activity, with the illuminance phase preceding the locomotion phase. This circadian rhythm emerged during the first 3 d and was maintained for at least 30 d. Compared to mice whose light cycle was controlled by other mice (in a separate chamber) or those maintained on a regular 12 h-light/12 h-dark cycle, mice that controlled their own light cycle exhibited fewer mid-sleep awakenings, lower levels of stress-related steroids, and improved cognitive performance. These emergent circadian rhythms provide insight into the evolution of ecological circadian dynamics and may inform the development of efficient lifestyles in humans.

show abstract

Thermosensitive receptors in neural stem cells link stress-induced hyperthermia to impaired neurogenesis via microglial engulfment

Abstract: Hyperthermia mediates social defeat stress–induced impairment of adult neurogenesis.

Cited by 16 publications

References 61 publications

Hyperthermia alters neurobehavior by affecting cell proliferation and neuronal survival in young male rats

Hyperthermia alters neurobehavior by affecting cell proliferation and neuronal survival in young male rats

Microglial Responses to Stress-Induced Depression: Causes and Consequences

Rodent control of chamber brightness leads to the emergence of unique circadian rhythms

Contact Info

Product

Resources

About