Thermostability, pH stability and dye degrading activity of a bacterial laccase are enhanced in the presence of Cu2O nanoparticles

Mukhopadhyay, Arka; Dasgupta, Anjan Kumar; Chakrabarti, Krishanu

doi:10.1016/j.biortech.2012.09.087

Cited by 49 publications

(28 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…4a). Like previous observations of 90% phenol degradation using purified HRP, we achieved an efficiency of 96% polymerization using the immobilized HRP [19,40,41]. The efficiency of phenol reduction can be enhanced by either increasing the enzyme concentration or by increasing the incubation time.…”

Section: Efficient Removal Of Phenol and P-chlorophenol From Spiked Msupporting

confidence: 76%

A Simple and Efficient Method for Removal of Phenolic Contaminants in Wastewater Using Covalent Immobilized Horseradish Peroxidase

Ahirwar¹,

Sharma²,

Singh³

et al. 2017

JMSE-B

View full text Add to dashboard Cite

Abstract:In the light of the increasing burden of pollutants in major rivers and stringent environmental legislation, adaptation to eco-friendly treatment approaches is desperately required to decontaminate industrial effluents before its discharge to rivers and other fresh water-bodies. In present study, we have designed a simple and efficient method for removal of phenols from effluent wastewater using an immobilized preparation of HRP (horseradish peroxidase). The enzyme was isolated in bulk amount from the roots of the Armoracia rusticana and covalently immobilized to polycarbonate supports using a photolabile linker FNAB (1-fluoro-2-notro-4-azidobenzene). The immobilized enzyme showed enhanced storage stability and reusability. The immobilized HRP was subsequently used for degradation of phenols in sewage and spiked wastewater. The phenol content of spiked wastewater was reduced to 93% in the 3 L reactor following treatment with immobilized HRP and H 2 O 2 . The improvement in the quality of water upon treatment was reflected by the changes in pH, conductivity, TDS (total dissolved salts) and biodegradation of organic contents as indicated by 77% and 87% reduction in COD (chemical oxygen demand) and BOD (biochemical oxygen demand) respectively in the analyzed sample.

show abstract

Section: Efficient Removal Of Phenol and P-chlorophenol From Spiked Msupporting

confidence: 76%

A Simple and Efficient Method for Removal of Phenolic Contaminants in Wastewater Using Covalent Immobilized Horseradish Peroxidase

Ahirwar¹,

Sharma²,

Singh³

et al. 2017

JMSE-B

View full text Add to dashboard Cite

show abstract

“…The characteristics of laccase have been widely studied in fungi, especially white rot fungi, which have been regarded as the most efficient laccase producers and are used in industrial processes (Senthivelan et al 2016). However, fungi often grow slowly and require a long time to produce laccase; also, most of their laccases have the drawbacks of low thermostability and a limited pH-activity range (pH 4.0 to 6.0) (Majeau et al 2010;Mukhopadhyay et al 2013). The majority of fungal laccases usually rapidly drop their activity at temperatures above 60 °C and the half-life range of their activities at 60 °C was reported as 0.3 to 2 h (Hildén et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Characterization of a Novel Laccase-producing Bacillus sp. A4 and its Application in Miscanthus Degradation

Guo¹,

Lin²,

Wang³

et al. 2017

BioResources

View full text Add to dashboard Cite

Bacillus sp. A4 exhibiting laccase production was isolated from forest soil. Its laccase secreted into a LB medium exhibited a maximum activity of 3.9 U mg -1 protein at the optimal temperature (37 °C) and pH (6.0). The purified laccase of Bacillus sp. A4 demonstrated a low molecular mass of 33 kDa, and its optimal temperature and pH were 40 °C and 4.6, respectively, when using ABTS as a substrate. The activity of the purified laccase was significantly increased in the presence of Cu 2+ , methanol, and ethanol, but it was totally inhibited by L-cysteine. The laccase production of this strain was markedly stimulated when the strain was incubated with 0.5% different lignocellulosic biomasses. The highest activity of laccase (22.6 U mg -1 protein) was obtained in using algal biomass. This new strain efficiently decreased the lignin content of lignocellulose biomasses after 9 d of incubation at 37 °C, especially lignin from grasses. Further analysis showed that, compared to that of all tested biomasses, the new strain was a more efficient decomposer of the lignin of Miscanthus, which exhibited much more lignin loss and cell wall structure destruction in a short span of time. Therefore, the potential use of this strain could be advantageous for using lignin in Miscanthus for industrial processes.

show abstract

“…We had reported that the NP imparted both thermostability and enhanced activity to the enzyme (Mukhopadhyay et al, 2012(Mukhopadhyay et al, , 2013a. However, the stability of the psychrophilic laccase at a very low temperature (4°C) and the remarkable resilience to freeze-thaw cycles, following NP or NP + SWNT treatment was a unique and novel finding.…”

Section: Retention Of Activity By Laccase System As a Dependent Of Tementioning

confidence: 95%

“…In recent work reported from our laboratory, the addition of cofactors (metal) in nano form greatly enhanced the activity and thermal stability of pectate lyase and laccase (Mukhopadhyay et al, 2012(Mukhopadhyay et al, , 2013a. Such addition also restored the functional properties of a functionally impaired pectate lyase (Dutta et al, 2014).…”

Section: Introductionmentioning

confidence: 90%

Enhanced functionality and stabilization of a cold active laccase using nanotechnology based activation-immobilization

Mukhopadhyay

Dasgupta

Chakrabarti

2015

Bioresource Technology

Self Cite

View full text Add to dashboard Cite

Thermostability, pH stability and dye degrading activity of a bacterial laccase are enhanced in the presence of Cu2O nanoparticles

Cited by 49 publications

References 29 publications

A Simple and Efficient Method for Removal of Phenolic Contaminants in Wastewater Using Covalent Immobilized Horseradish Peroxidase

A Simple and Efficient Method for Removal of Phenolic Contaminants in Wastewater Using Covalent Immobilized Horseradish Peroxidase

Characterization of a Novel Laccase-producing Bacillus sp. A4 and its Application in Miscanthus Degradation

Enhanced functionality and stabilization of a cold active laccase using nanotechnology based activation-immobilization

Contact Info

Product

Resources

About