Thermostable amylolytic enzymes of thermophilic microorganisms from deep-sea hydrothermal vents

Legin, Estelle; Delbarre-Ladrat, Christine; Godfroy, Anne; Barbier, Georges; Duchiron, Francis

doi:10.1016/s0764-4469(97)80874-8

Cited by 24 publications

(16 citation statements)

References 15 publications

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“…Strain ST549 has been screened for total amylolytic activities (i.e. K-amylase, K-glucosidase and pullulanase), however, none of these activities were detected in this strain [21]. P. abyssi ST549 appeared to be unable to use disaccharides and this is consistent with previous growth studies which showed that P. abyssi ST549 grew preferentially on proteinaceous substrates (Godfroy, data not shown).…”

Section: Discussionsupporting

confidence: 85%

Physiology and continuous culture of the hyperthermophilic deep-sea vent archaeon Pyrococcus abyssi ST549

Godfroy

2000

FEMS Microbiology Letters

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The deep-sea vent archaeon Pyrococcus abyssi strain ST549 was grown in batch cultures in closed bottles and by continuous culture in a gas-lift bioreactor, both in the presence and in the absence of elemental sulfur. Growth on carbohydrates, proteinaceous substrates and amino acids was investigated. The disaccharides maltose and cellobiose were shown not to be able to enhance growth suggesting that P. abyssi ST549 is unable to use them as carbon sources. By contrast, proteinaceous substrates such as peptone and brain heart infusion were shown to be very good substrates for the growth of P. abyssi ST549 and allowed growth at high steady-state cell densities in continuous culture. Growth on brain heart infusion was shown to require additional nutrients when sulfur was not present in the culture medium. Growth on amino acids only took place in the presence of sulfur. These results indicate that sulfur plays an important role in the metabolism and energetics of P. abyssi ST549. ß

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Section: Discussionsupporting

confidence: 85%

Physiology and continuous culture of the hyperthermophilic deep-sea vent archaeon Pyrococcus abyssi ST549

Godfroy

2000

FEMS Microbiology Letters

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“…Studies carried out by Skovgaard and Rosendahl [9] revealed the production of amylases by Fusarium oxysporum from different habitats in culture media. According to Legin et al, [10], Thermococcus hydrothermalis and Thermococcus fumicolans isolated from deep sea were amylase producers. Sporothichum thermophile Apinis produce amylase with activity reaching approximately 45.2 M-Units after 5 days of growth [11].…”

Section: Open Access Http://scidocorg/ijmbgphpmentioning

confidence: 98%

Use of Tropical Strains: Aspergillus vadensis and Aspergillus oryzae as Producers of α-Amylases in Biotechnological Practice

Adejuwon¹,

VA²

2017

IJMBG

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“…Recently, Thermococcus AL662 strain, identified as Thermococcus hydrothermalis [3], was found incapable of growth when starch was used as carbon source. However, it has been shown that it was able to produce a low level of α‐amylase activity [4]. The purification, and other information regarding this enzyme are unavailable.…”

Section: Introductionmentioning

confidence: 99%

Cloning and expression of an Î±-amylase encoding gene from the hyperthermophilic archaebacteriumThermococcus hydrothermalisand biochemical characterisation of the recombinant enzyme

Lévêque¹,

Haye²,

Belarbi³

2000

FEMS Microbiology Letters

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An alpha-amylase encoding gene from the extremely thermophilic Archaea Thermococcus hydrothermalis was cloned and expressed in Escherichia coli. The encoded alpha-amylase possesses molecular characteristics specific to the Archaea, especially from Pyrococcus species, with biochemical characteristics of the alpha-amylases from Thermococcus. The gene is 1374 bp long and encodes a protein of 457 amino acids composed of a 22 amino acid putative signal peptide and a 435 amino acid mature protein (calculated molecular mass 49236 Da). The T. hydrothermalis recombinant alpha-amylase is optimally active at 75-85 degrees C and at pH 5.0-5.5.

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Thermostable amylolytic enzymes of thermophilic microorganisms from deep-sea hydrothermal vents

Cited by 24 publications

References 15 publications

Physiology and continuous culture of the hyperthermophilic deep-sea vent archaeon Pyrococcus abyssi ST549

Physiology and continuous culture of the hyperthermophilic deep-sea vent archaeon Pyrococcus abyssi ST549

Use of Tropical Strains: Aspergillus vadensis and Aspergillus oryzae as Producers of α-Amylases in Biotechnological Practice

Cloning and expression of an Î±-amylase encoding gene from the hyperthermophilic archaebacteriumThermococcus hydrothermalisand biochemical characterisation of the recombinant enzyme

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