Thermostable cellulase production of Aspergillus fumigatus Z5 under solid-state fermentation and its application in degradation of agricultural wastes

Liu, Dongyang; Zhang, Ruifu; Yang, Xinlin; Wu, Hong‐sheng; Xu, Dawei; Tang, Zhu; Shen, Qirong

doi:10.1016/j.ibiod.2011.04.005

Cited by 146 publications

(80 citation statements)

References 41 publications

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“…Previous studies have shown that organic sources greatly improved the production of enzymes (Dong et al, 2005) by P. ostreatus (Mikiashvili et al, 2006) as well as fungi L. edodes, P. chrysosporium in the activity of MnP (Kaal et al, 1995). Li et al (2008) noted that the use of solid state fermentation of the enzymatic activities produced MnP (9.67 UI.L -1 ) as compared to the liquid fermentation (0.044 UI.L -1 ) especially with agroindustrial residues, which allows the concentrated product (Liu et al, 2011). Hatakka (2001) reported that the degradation of lignin by L. edodes occurred during the secondary metabolism and nitrogen reached high levels of lignocellulosic degradation.…”

Section: Treatments Daysmentioning

confidence: 99%

Influence of nitrogen sources on the enzymatic activity and grown by Lentinula edodes in biomass Eucalyptus benthamii

et al. 2015

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Lignocellulose is the most abundant environmental component and a renewable organic resource in soil. There are some filamentous fungi which developed the ability to break down and use cellulose, hemicellulose and lignin as an energy source. The objective of this research was to analyze the effect of three nitrogen resources (ammonium sulfate, saltpetre, soybean) in the holocellulolitic activity of Lentinula edodes EF 50 using as substrate sawdust E. benthamii. An experimental design mixture was applied with repetition in the central point consisting of seven treatments (T) of equal concentrations of nitrogen in ammonium sulfate, potassium nitrate and soybean. The enzymatic activity of avicelase, carboxymetilcellulase, β-glucosidase, xylanases and manganese peroxidase was determined. The humidity, pH, water activity (a w ) and qualitative analysis of mycelial growth in 8 times of cultivation were evaluated. The results showed negative effect on enzyme production in treatments with maximum concentration of ammonium sulfate and potassium nitrate. The treatments with cooked soybean flour expressed higher enzymatic activities in times of 3, 6 and 9 days of culture, except in the activity of manganese peroxidase. The highest production was observed in the treatment with ammonium sulfate, and soybean (83.86 UI.L -1 ) at 20 days of cultivation.

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Section: Treatments Daysmentioning

confidence: 99%

Influence of nitrogen sources on the enzymatic activity and grown by Lentinula edodes in biomass Eucalyptus benthamii

et al. 2015

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“…Wheat bran is also a mostly available waste which comprises about 46% of polysaccharides (glucuronoarabinoxylans, cellulose, mixed linked (1-3)(1-4)-β-D-glucans and starch), proteins (15-22%) and lignin (4-8%) (SOUZA et al, 2012). These substrates have been tested as carbon sources for the production of cellulases and hemicellulases by fungal strains (LIU et al, 2011;DELABONA et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Substract and temperature effect on xylanase production by aspergillus fumigatus using low cost agricultural wastes

Gomes¹,

Santos²,

Franciscon³

et al. 2016

Biosci. J.

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This study reports the optimization of xylanase production under solid state fermentation (SSF) by a thermotolerant Aspergillus fumigatus strain (SCB4) isolated from sugarcane bagasse piles of Brazilian Cerrado. Different combinations of low-cost agricultural byproducts in SSF were evaluated: sugarcane bagasse and wheat bran (1:1), sugarcane bagasse and corn straw (1:1) and only sugarcane bagasse. The enzyme biosynthesis by SSF was carried out at different temperatures (40, 45, 50 and 55 o C). The maximum levels of xylanase activity were obtained after 24 h at 45 °C using a culture medium containing sugarcane bagasse and wheat bran (1:1). Under optimal conditions, the fungal culture produced 574 U g -1 of xylanase (units/g of dry substrate). The crude enzyme showed optimal activity at 60 °C and pH 4.5. It exhibited thermostability up to 55 °C, wide range of pH stability and tolerance to ethanol, xylose and glucose. The physicochemical properties shown by this enzyme are appropriate for its application in hydrolysis of lignocellulosic residues for ethanol production and other bioproducts.

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“…are the most commonly used substrates for the production of cellulase. For instance, Liu et al [57] investigated the production of cellulase on different lignocellulosic substrates such as straws of rice, wheat and cotton, corn stover and corncob using A. fumigatus Z5; of them, the corn comb supported the maximum production of endoglucanase. P. echinulatum 9A02S1 showed the maximum production of cellulolytic enzymes on the medium containing a mixture of pretreated sugarcane bagasse and wheat bran [58].…”

Section: Solid-state Fermentationmentioning

confidence: 99%

An Overview on Fungal Cellulases with an Industrial Perspective

Sreedharan¹

2016

J Nutr Food Sci

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Lignocellulose is the most abundant biopolymer available on earth. Hydrolysis of lignocellulose into fermentable sugars, sugar acids and phenolics is the pre-requisite for its successful exploitation as substrates for the large scale production of industrially significant value added products. Though remarkable collection of lignocellulolytic microorganisms has been brought to limelight, only a few especially fungi have been studied extensively as they secrete copious lignocellulolytic enzymes extracellularly. Enzymatic hydrolysis of lignocelluloses is carried out by a group of enzymes viz., cellulases, hemicellulases, ligninases in unison or individually, and are collectively known as lignocellulolytic enzymes. In this light of this background, followed by a short introduction on lignocellulose, lignocellulolytic enzymes and mainly focused on fungal cellulase, this review discusses recent approaches on the production of cellulase by submerged and solid-state fermentation strategies; current knowledge on the purification, characterisation of cellulase; and finally concluded with detailed industrial applications of fungal cellulase.

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Thermostable cellulase production of Aspergillus fumigatus Z5 under solid-state fermentation and its application in degradation of agricultural wastes

Cited by 146 publications

References 41 publications

Influence of nitrogen sources on the enzymatic activity and grown by Lentinula edodes in biomass Eucalyptus benthamii

Influence of nitrogen sources on the enzymatic activity and grown by Lentinula edodes in biomass Eucalyptus benthamii

Substract and temperature effect on xylanase production by aspergillus fumigatus using low cost agricultural wastes

An Overview on Fungal Cellulases with an Industrial Perspective

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