1994
DOI: 10.1099/00221287-140-6-1457
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Thermostable extracellular peroxidases from Streptomyces thermoviolaceus

Abstract: Streptomyces thermoviolsceus is a thermophil ic actinomycete that was found t o produce relatively large amounts of extracellular peroxidase activity when grown on xylan as primary carbon source. The activity was due to multiple isoforms of peroxidase, of which two, designated P-3 and P-5, were predominant. The two proteins were purified to homogeneity by a combination of ultraf iltration, ammonium sulphate precipitation, anionexchange chromatography, gel filtration and preparative gel electrophoresis. The per… Show more

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Cited by 27 publications
(20 citation statements)
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“…Naturally thermostable peroxidases have been described and include soybean peroxidase (McEldoon and Dordick, 1996), extracellular peroxidases from Streptomyces thermoviolaceus (Iqbal et al, 1994), and an intracellular peroxidase from Bacillus sphaericus (Apitz and van Pee, 2000). Relatively little is known, however, of the reactivities and substrates of these enzymes compared to the widely used, but less thermostable, horseradish peroxidase.…”
Section: Comparison To Other Peroxidasesmentioning
confidence: 99%
“…Naturally thermostable peroxidases have been described and include soybean peroxidase (McEldoon and Dordick, 1996), extracellular peroxidases from Streptomyces thermoviolaceus (Iqbal et al, 1994), and an intracellular peroxidase from Bacillus sphaericus (Apitz and van Pee, 2000). Relatively little is known, however, of the reactivities and substrates of these enzymes compared to the widely used, but less thermostable, horseradish peroxidase.…”
Section: Comparison To Other Peroxidasesmentioning
confidence: 99%
“…An authentic type III heme peroxidase (members of the catalase-peroxidase family are classified as type I) has yet to be demonstrated in a prokaryote. c Turnover for the T. fusca protein is ϳ14 times that of free Cu, but the protein may be associated with up to 20 mol of Cu/mol of protein (17).…”
Section: Fusca Pseudoperoxidase and Porphyrin Excretion 4517mentioning
confidence: 99%
“…Since the discovery of extracellular lignindegrading heme peroxidases of wood-rotting fungi, considerable effort has been expended in searching for analogous enzymes in the cellulolytic actinomycetes (9,22,24). Indeed, some proteins secreted by the actinomycetes Streptomyces thermoviolaceus (17), Streptomyces viridosporus T7A (6,26,27), and Thermomonospora fusca BD25 (recently reclassified as Thermobifida fusca [35]) (3,28) have low peroxidase activity and have been isolated and partially characterized. The peroxidase-like proteins from S. thermoviolaceus and S. viridosporus T7A were assigned as heme peroxidases on the basis of their optical spectra.…”
mentioning
confidence: 99%
“…The production of extracellular peroxidase increased significantly during the growth phase of culture (1-3 d). This period falling within the range frequently reported for actinobacteria (2 d to 3 d), including Streptomyces thermoviolaceus (Iqbal et al, 1994), Thermomonospora fusca BD25 (Rob et al, 1996;Tuncer et al, 1999), Streptomyces avermitilis UAH30 (Rob et al, 1997), Streptomyces viridosporus T7A (Zerbini et al, 1999), and Streptomyces albus ATCC 3005 (Antonopoulos et al, 2001). After the first 3 d of incubation, activity of peroxidase declined until 10 d of incubation.…”
Section: Resultsmentioning
confidence: 83%
“…The reaction mixture (total volume 1 mL) contained equal volumes (0.2 mL) of potassium phosphate buffer (100 mmol/L, pH 8.0) 2,4-DCP (25 mmol/L), 4-aminoantipyrine (16 mmol/L), enzyme solution and H 2 O 2 (50 mmol/L). The reaction was initiated with the addition of H 2 O 2 and the reaction was monitored at 30 C for 1 min at a wavelength of 510 nm (Ramachandra et al, 1988;Iqbal et al, 1994). One unit (U) of peroxidase activity was defined as the amount of enzyme required for an increase in absorbance of 1 unit per minute.…”
Section: Preparation Of Ball-milled Wheat Straw (Bmws)mentioning
confidence: 99%