Thermostable xylanases produced at 37°C and 45°C by a thermotolerant Aspergillus strain

Castro, Laura P Mendicuti; Trejo-Aguilar, Blanca; Osorio, Guillermo Aguilar

doi:10.1111/j.1574-6968.1997.tb10177.x

Cited by 11 publications

(9 citation statements)

References 9 publications

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“…4A). Similar results were reported by Khasin et al (1993) for Bacillus steathermophilus T6 xylanases and by Mendicuti-Castro et al (1997) for Aspergillus strain xylanases. When the xylanase was preincubated at different times (0-75 min) at various temperatures (Fig.…”

Section: Properties and Partial Purification Of The Xylanases Producesupporting

confidence: 91%

Induction, Properties and Application of Xylanase Activity From Sclerotinia Sclerotiorum S2 Fungus

Olfa

Mejri

Smaali

et al. 2007

J Food Biochemistry

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Extracellular xylanase activity was produced in a submerged culture by Sclerotinia sclerotiorum S2 fungus, on wheat bran as an inducing substrate. The enzyme was partially purified and biochemically characterized. The optimum pH and temperature for the activity were 5.5 and 65C, respectively. The xylanase was stable over a pH range of 4.0–9.0 and retained more than 75% of its activity after incubation for 24 h without substrate. The enzyme was stable at 50C and 60C for 80 min of incubation at pH 5.0, while the half‐life was 20 min at 70C. The novel xylanase activity was useful as an aid in orange juice clarification. The clarification was observed with concomitant production of reducing sugars (0.350 mg/mL from juice) after 24 h of incubation of juice with the xylanase. Xylanase aided juice clarification, which resulted in a 27% decrease in insoluble materials.

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Section: Properties and Partial Purification Of The Xylanases Producesupporting

confidence: 91%

Induction, Properties and Application of Xylanase Activity From Sclerotinia Sclerotiorum S2 Fungus

Olfa

Mejri

Smaali

et al. 2007

J Food Biochemistry

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“…A change in the intrinsic properties of the xylanase from Aspergillus FP-470 was also reported by Mendicuti-Castro [12]. In that case, a change from 37°C to 45°C resulted in a switch of optimum pH from 6.5 to 4.3 and a switch of temperature optimum from 80°C to 50°C.…”

Section: Discussionmentioning

confidence: 61%

Influence of temperature on the properties of the xylanolytic enzymes of the thermotolerant fungus Aspergillus phoenicis

Rizzatti

Sandrim

Jorge

et al. 2004

Journal of Industrial Microbiology and Biotechnology

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This study reports on the effects of growth temperature on the secretion and some properties of the xylanase and beta-xylosidase activities produced by a thermotolerant Aspergillus phoenicis. Marked differences were observed when the organism was grown on xylan-supplemented medium at 25 degrees C or 42 degrees C. Production of xylanolytic enzymes reached maximum levels after 72 h of growth at 42 degrees C; and levels were three- to five-fold higher than at 25 degrees C. Secretion of xylanase and beta-xylosidase was also strongly stimulated at the higher temperature. The optimal temperature was 85 degrees C for extracellular and 90 degrees C for intracellular beta-xylosidase activity, independent of the growth temperature. The optimum temperature for extracellular xylanase increased from 50 degrees C to 55 degrees C when the fungus was cultivated at 42 degrees C. At the higher temperature, the xylanolytic enzymes produced by A. phoenicis showed increased thermostability, with changes in the profiles of pH optima. The chromatographic profiles were distinct when samples obtained from cultures grown at different temperatures were eluted from DEAE-cellulose and Biogel P-60 columns.

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“…In addition, A. terreus bioactivities recorded as 14.9 and 26.5 lg/ml against V. ordalli and V. angularuim respectively, showed a wide tolerance of temperature above 30°C. Comparing with these results, the optimum temperature for the marine V. ramulosa was ranged from 24-26°C with maximum productivity of 6.2 lg/ml [17]. Vishwanatha et al [30] showed the culture flasks of A. oryzae MTCC 5341 for maximum acid protease production at Fig.…”

Section: Effect Of Ph and Temperature On The Productivitymentioning

confidence: 71%

“…The filtrate of the precultured A. terreus was divided into five portions and exposed separately to different temperature degrees (30, 40, 60, 80 and 100°C) for 30 min. The thermo-stability was assessed as a function of their bioactivity against the tested pathogens [17].…”

Section: Thermo-stability Testmentioning

confidence: 99%

Antimicrobial Agents Produced by Marine Aspergillus terreus var. africanus Against Some Virulent Fish Pathogens

Barakat

Gohar

2012

Indian J Microbiol

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Screening of fungal isolates collected from different locations of Alexandria coast, Egypt, was carried out to obtain new biologically active metabolites against some virulent fish pathogens (Edwardsiella tarda, Aeromonas hydrophila, Vibrio ordalli and Vibrio angularuim). Among 26 fungal isolates, Aspergillus terreus var. africanus was identified as the most potent isolate. Production of the bioactive material was optimized using response surface methodology including fermentation media, incubation period, temperature, pH, and thermo-stability. Spectral properties of the gas chromatography/mass spectrum of the ethyl acetate crude extract were determined. Partially purified components of the crude extract were chromatographically separated and bioassayed. Out of ten separated compounds, five were with considerable antibacterial agent. The bio-toxicity of crude showed a slight toxicity against the brine shrimp Artemia salina (LC50 = 1,500 μg/l). Antibacterial activity of the crude was compared with some known standard antibiotics and found to be superior over many where its MIC against some pathogen reached 1 μg/ml.

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Thermostable xylanases produced at 37°C and 45°C by a thermotolerant Aspergillus strain

Cited by 11 publications

References 9 publications

Induction, Properties and Application of Xylanase Activity From Sclerotinia Sclerotiorum S2 Fungus

Induction, Properties and Application of Xylanase Activity From Sclerotinia Sclerotiorum S2 Fungus

Influence of temperature on the properties of the xylanolytic enzymes of the thermotolerant fungus Aspergillus phoenicis

Antimicrobial Agents Produced by Marine Aspergillus terreus var. africanus Against Some Virulent Fish Pathogens

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