Thermostable α-amylase production by Bacillus subtilis entrapped in calcium alginate gel capsules

Konsoula, Zoi; Liakopoulou-Kyriakides, M.

doi:10.1016/j.enzmictec.2005.12.002

Cited by 70 publications

(48 citation statements)

References 17 publications

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“…The immobilized cells retained almost 96% of their initial cyanide removal efficiency during the first five batches, when each cycle was continued for 10 h. Under these conditions, the entrapped cells system maintained 64% of its initial activity after 8 successive batches. Similar observations were made by Adinarayana et al (2004), Konsoula et al (2006) and Zheng et al (2009).…”

Section: Reusability Of Gel Matrixsupporting

confidence: 76%

Cyanide degradation by immobilized cells of Rhodococcus UKMP-5M

et al. 2012

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Anthropogenic sources contribute to the bulk presence of cyanide, which causes substantial health and environmental concerns. A petroleum-contaminated soil isolate, Rhodococcus UKMP-5M has been verified to efficiently degrade high concentration of cyanide in the form of KCN in our previous study. In order to enhance the cyanide-degrading ability of this bacterium, different encapsulation matrices were screened to immobilize cells of Rhodococcus UKMP-5M for degradation of cyanide. It was revealed that the biocatalyst activity and bead mechanical strength improved significantly when calcium alginate encapsulation technique was employed as compared to free cells. The results also indicated that the immobilized cell system could tolerate a higher level of KCN concentration and were able to support a higher biomass density. In addition, the embedded cells retained almost 96% of their initial cyanide removal efficiency during the first five batches and the entrapped cell system maintained 64% of its initial activity after eight successive batches. The encapsulated beads could be easily recovered from the production medium and reused for up to five batches without significant losses of cyanide-degrading ability, which proved to be advantageous from an economic point of view. From this study, it could be inferred that the novel Rhodococcus UKMP-5M strain demonstrated high cyanide-degrading ability and the optimized calcium alginate immobilization technique provided a promising alternative for practical application of large scale remediation of cyanide-bearing wastewaters.

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Section: Reusability Of Gel Matrixsupporting

confidence: 76%

Cyanide degradation by immobilized cells of Rhodococcus UKMP-5M

et al. 2012

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“…O alginato é um dos suportes mais utilizados para a imobilização de células microbianas inteiras por ser uma metodologia simples e barata; além de ser uma técnica reprodutível que utiliza condições suaves durante o processo de imobilização [65][66][67][68][69][70][71] . Os alginatos são heteropolímeros lineares de ácidos carboxílicos compostos de subunidades monoméricas de β-D-ácido manurônico (M) e α-Lácido gulurônico (G) interligados por ligações 1,4-glicosídicas.…”

Section: Aprisionamento No Interior De Um Suporte Porosounclassified

Produção de isomaltulose, um substituto da sacarose, utilizando glicosiltransferase microbiana

Kawaguti¹,

Sato²

2008

Quím. Nova

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“…Additionally, immobilized cell technology can reduce processing time and downstream processing as it can act as a biomass separator. A number of previous studies have attempted to investigate the influence of immobilization on cell growth and metabolic activity (Duran-Paramo et al 2000;Argirakos et al 1992;Dobreva et al 1998;Konsoula and Liakopoulou-Kyriakides 2006). The selection of the materials used for the matrices is of the utmost importance because it determines their characteristics.…”

Section: Introductionmentioning

confidence: 99%

Production of α-Amylase by Immobilized Bacillus Subtilis in Polymeric PolyHIPE Matrix

Jimat

Harwood

Akay

2013

Developments in Sustainable Chemical and Bioprocess Technology

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The microcellular polymer known as polyHIPE polymer (PHP), with modified physico-chemical characteristics, was developed as a cell matrix for the immobilization of the starch-degrading bacterium, Bacillus subtilis. Suspension of B. subtilis spores was inoculated into a synthesized PHP matrix which is chemically modified, and this PHP matrix was named sulphonated PHPs (SPHPs). These inoculated spores were then activated by supplying continuously well-aerated culture medium (LB medium) and placed in a 37°C constant temperature room for 24-h incubation. The growth and enzyme productivity data were evaluated and compared. Three different pore and interconnect sizes of SPHPs were evaluated: 42.0 ± 0.61, 36.0 ± 0.50 and 30.0 ± 0.64 lm. The collected samples obtained from the 24-h cultivation were used to determine a-amylase productivity and the loss of cells from the matrices. The morphology, viability and proliferation of the immobilized cells on PHP matrices were observed by scanning electron microscopy (SEM). The SPHP with a pore size of 36.0 lm performed better with respect to the production of a-amylase and the penetration of cells through the whole matrix compared to other SPHPs. The data showed that the total productivity of a-amylase enzyme produced by immobilized cells (on the basis of SPHP volume) was 7.6-fold higher than the batch cell culture. However, if productivity was determined on the basis of used total volume of nutrient medium, that of the immobilized cells was relatively low compared to batch cell culture.

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Thermostable α-amylase production by Bacillus subtilis entrapped in calcium alginate gel capsules

Cited by 70 publications

References 17 publications

Cyanide degradation by immobilized cells of Rhodococcus UKMP-5M

Cyanide degradation by immobilized cells of Rhodococcus UKMP-5M

Produção de isomaltulose, um substituto da sacarose, utilizando glicosiltransferase microbiana

Production of α-Amylase by Immobilized Bacillus Subtilis in Polymeric PolyHIPE Matrix

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