Thermotolerance Responses in Ripening Berries of Vitis vinifera L. cv Muscat Hamburg

Carbonell‐Bejerano, Pablo; María, Eva Santa; Torres‐Pérez, Rafael; Royo, C.; Lijavetzky, Diego; Bravo, Gema; Aguirreolea, Jone; Sánchez‐Díaz, Manuel; Antolín, M. Carmen; Martı́nez-Zapater, José M.

doi:10.1093/pcp/pct071

Cited by 124 publications

(114 citation statements)

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“…This is in agreement with general observations by viticulturists that moderately warm temperatures favor sugar accumulation during ripening [21,112,114] leading to wines higher in alcohol in warm climates and seasons. In a previous study on fruiting cuttings ABA levels were significantly increased by high temperature after 45 but not after 14 days of post-véraison heat treatment [38]. This is consistent with our results where the repression of ABA synthesis genes in berries at véraison is inversed at the more developed stages.…”

Section: Resultssupporting

confidence: 93%

“…Similar categories were shown to be modified by temperature in a previous study with fruiting cuttings [38]. The short heat stress period of 2 h in the present study presumably enhanced the induction of these transcripts in which over-expression was not observed with longer temperature treatments in other studies where plants started to adapt to their changed conditions [66-68].…”

Section: Resultssupporting

confidence: 85%

“…Carbonell-Bejerano et al, 2013 [38] observed the induction of an osmotin transcript ( VIT_02s0025g04340 ) indicating its putative function in activating osmoprotection in response to elevated temperatures. Conversely, in this study, this transcript and three other osmotin-coding genes were down-regulated by heat stress at VS during the day, which brings into question the actual role of this gene in response heat stress in grapevine fruits.…”

Section: Resultsmentioning

confidence: 99%

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Day and night heat stress trigger different transcriptomic responses in green and ripening grapevine (vitis vinifera) fruit

et al. 2014

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BackgroundGlobal climate change will noticeably affect plant vegetative and reproductive development. The recent increase in temperatures has already impacted yields and composition of berries in many grapevine-growing regions. Physiological processes underlying temperature response and tolerance of the grapevine fruit have not been extensively investigated. To date, all studies investigating the molecular regulation of fleshly fruit response to abiotic stress were only conducted during the day, overlooking possible critical night-specific variations. The present study explores the night and day transcriptomic response of grapevine fruit to heat stress at several developmental stages. Short heat stresses (2 h) were applied at day and night to vines bearing clusters sequentially ordered according to the developmental stages along their vertical axes. The recently proposed microvine model (DRCF-Dwarf Rapid Cycling and Continuous Flowering) was grown in climatic chambers in order to circumvent common constraints and biases inevitable in field experiments with perennial macrovines. Post-véraison berry heterogeneity within clusters was avoided by constituting homogenous batches following organic acids and sugars measurements of individual berries. A whole genome transcriptomic approach was subsequently conducted using NimbleGen 090818 Vitis 12X (30 K) microarrays.ResultsPresent work reveals significant differences in heat stress responsive pathways according to day or night treatment, in particular regarding genes associated with acidity and phenylpropanoid metabolism. Precise distinction of ripening stages led to stage-specific detection of malic acid and anthocyanin-related transcripts modulated by heat stress. Important changes in cell wall modification related processes as well as indications for heat-induced delay of ripening and sugar accumulation were observed at véraison, an effect that was reversed at later stages.ConclusionsThis first day - night study on heat stress adaption of the grapevine berry shows that the transcriptome of fleshy fruits is differentially affected by abiotic stress at night. The present results emphasize the necessity of including different developmental stages and especially several daytime points in transcriptomic studies.

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Section: Resultssupporting

confidence: 93%

Section: Resultssupporting

confidence: 85%

Section: Resultsmentioning

confidence: 99%

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Day and night heat stress trigger different transcriptomic responses in green and ripening grapevine (vitis vinifera) fruit

et al. 2014

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“…Yan et al (2014) isolated a full-length MBF1 cDNA sequence (VvMBF1) from V. vinifera 3 Vitis amurensis that was up-regulated in the leaves of grape plants following both drought and abscisic acid treatments. Carbonell-Bejerano et al (2013) reported that berry MBF1a and MBF1c were up-regulated under high temperature when grape 'Muscat Hamburg' plants growing from cuttings were exposed to two different environmental regimes, 20°C/15°C (day/night) and 30°C/25°C (day/night), at the onset of ripening (veraison). Wang and Li (2006a) reported that exogenous SA pretreatment decreased thiobarbituric acidreactive substances content and relative electrolyte leakage in grape leaves under heat stress, indicating that SA can induce intrinsic heat tolerance in grape.…”

Section: Some Important Transcript Factors Could Be Involved In Heat mentioning

confidence: 99%

“…Transcriptomic analysis of grape leaves under heat stress and subsequent recovery showed that the effect of heat stress and recovery on grape appears to be associated with multiple processes and mechanisms, including stress-related genes, transcription factors, and metabolism (Liu et al, 2012). Carbonell-Bejerano et al (2013) revealed that the establishment of a thermotolerance response in berries under high temperatures was marked by the induction of HSPs and the repression of transmembrane transporter-encoding transcripts using the GrapeGen GeneChip. George et al (2015) reported on proteome changes in cv Cabernet Sauvignon grape cells exposed to sudden high-temperature stresses, with more than 2,000 proteins identified and quantified using spectral counting of the entire data set.…”

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confidence: 99%

Integrating Omics and Alternative Splicing Reveals Insights into Grape Response to High Temperature

et al. 2017

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Heat stress is one of the primary abiotic stresses that limit crop production. Grape (Vitis vinifera) is a cultivated fruit with high economic value throughout the world, with its growth and development often influenced by high temperature. Alternative splicing (AS) is a widespread phenomenon increasing transcriptome and proteome diversity. We conducted high-temperature treatments (35°C, 40°C, and 45°C) on grapevines and assessed transcriptomic (especially AS) and proteomic changes in leaves. We found that nearly 70% of the genes were alternatively spliced under high temperature. Intron retention (IR), exon skipping, and alternative donor/acceptor sites were markedly induced under different high temperatures. Among all differential AS events, IR was the most abundant up-and down-regulated event. Moreover, the occurrence frequency of IR events at 40°C and 45°C was far higher than at 35°C. These results indicated that AS, especially IR, is an important posttranscriptional regulatory event during grape leaf responses to high temperature. Proteomic analysis showed that protein levels of the RNA-binding proteins SR45, SR30, and SR34 and the nuclear ribonucleic protein U1A gradually rose as ambient temperature increased, which revealed a reason why AS events occurred more frequently under high temperature. After integrating transcriptomic and proteomic data, we found that heat shock proteins and some important transcription factors such as MULTIPROTEIN BRIDGING FACTOR1c and HEAT SHOCK TRANSCRIPTION FACTOR A2 were involved mainly in heat tolerance in grape through up-regulating transcriptional (especially modulated by AS) and translational levels. To our knowledge, these results provide the first evidence for grape leaf responses to high temperature at simultaneous transcriptional, posttranscriptional, and translational levels.Heat stress is one of the main abiotic stresses limiting crop production worldwide. Global warming is predicted to be accompanied by more frequent and powerful extreme temperature events (Lobell et al., 2008). Therefore, a better understanding of the response of plants to heat stress is essential for improving their heat tolerance. In general, heat stress is a complex function of intensity (temperature in degrees), duration, and rate of increase in temperature. At very high temperatures, a catastrophic collapse of cellular organization may occur within minutes, which results in severe cellular injury and even cell death (Wahid et al., 2007). At moderately high temperatures, direct injuries include protein denaturation and aggregation and the increased fluidity of membrane lipids. Indirect or slower heat injuries include inactivation of enzymes, inhibition of protein synthesis, protein degradation, and loss of membrane integrity. These injuries eventually lead to a decline of net photosynthetic rate, reduced ion flux, production of toxic compounds and reactive oxygen species, and inhibition of growth. Actually, plants are not passively damaged by heat stress but respond to temperature changes by r...

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Grapevine abiotic and biotic stress genomics and identification of stress markers

Grimplet

2015

Grapevine in a Changing Environment

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Thermotolerance Responses in Ripening Berries of Vitis vinifera L. cv Muscat Hamburg

Abstract: 21 22 23 24 25 C LR-E LR-E+K LR-W LR-W+K 22,32c 23,22abc 23,07bc 24,25a 24,20ab) %

Cited by 124 publications

References 96 publications

Day and night heat stress trigger different transcriptomic responses in green and ripening grapevine (vitis vinifera) fruit

Day and night heat stress trigger different transcriptomic responses in green and ripening grapevine (vitis vinifera) fruit

Integrating Omics and Alternative Splicing Reveals Insights into Grape Response to High Temperature

Grapevine abiotic and biotic stress genomics and identification of stress markers

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