Thermotolerant and Alcohol-Tolerant Yeasts Targeted to Optimize Hydrolyzation from Mango Peel for High Bioethanol Production

Somda, Marius K.; Savadogo, Aly; Ouattara, Cheik A. T.; Ouattara, Aboubakar S.; Traoré, Alfred S.

doi:10.3923/ajbkr.2011.77.83

Cited by 23 publications

(10 citation statements)

References 8 publications

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“…Bioethanol produced from renewable biomass, such as sugar, starch or lignocellulosic materials, is one of the alternative energy resources, which is both renewable and environmentally linked (7) . Tolerance to high temperatures and high ethanol concentrations are important properties of microorganisms of interest to industry (8) .The ability of yeast to produce ethanol depends on many factors such as strains, growth factors and optimum environmental conditions (9) .…”

Section: Introductionmentioning

confidence: 99%

Characterization of Stress Tolerant High Potential Ethanol Producing Yeast from Agro-Industrial Waste

Fakruddin¹

2013

AJBIO

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Bioethanol or biofuel as an alternative to fossil fuels has been expanded in the last few decades in the whole world. Use of bioethanol as a renewable transportation fuel will minimize the amounts of fossil-derived carbon dioxide (CO 2) to the Earth's atmosphere. Yeast is the most favorite organism for ethanol production because of its diverse substrate specificity and ease of production of ethanol under anaerobic condition. The main objective of this research work was to isolate & characterize stress tolerant, high potential ethanol producing yeast strains from agro industrial waste. In total 4 yeast isolates have been characterized on the basis of morphological and physico-chemical characters. Based on morphological appearance of vegetative cell under microscope, ascospore production, colony character and physicochemical characters all the strains was identified to be Yeast. Phylogenetic identification by DNA sequencing confirmed that the strain P is Saccharomyces Unisporus, strain C is Saccharomyces cerevisiae, strain T is Saccharomyces cerevisiae & strain DB2 is Candida piceae. Most of the strains were thermotolerant, pH tolerant, ethanol tolerant as well as osmotolerant. They were resistant to cycloheximide at 0.0015g/100ml concentration, hydrogen peroxide (0.50%), Chloramphenicol (30µg/disc) but growth was inhibited in the presence of 1% acetic Acid. The strains P, C & T showed good Invertase activity & only the T strain was capable of producing killer toxin. They were capable of fermenting glucose, fructose, sucrose, amylose & trehalose. Ethanol producing capability of the strains was studied using sugarcane molasses as substrate. The bioethanol production capacity of the yeasts were found to be 15%, 14.5%, 12% & 8.15% for P, C, T & DB2 respectively at pH 6.0, 30 o C temperature in media with 5.5% initial reducing sugar concentration in shaking condition. Pilot scale ethanol production by P strain was 13.10%, C strain 11.15%, T strain 9.80% & DB2 strains 7.85% at 60 hours. These strains could be potential for ethanol production from cane molasses.

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Section: Introductionmentioning

confidence: 99%

Characterization of Stress Tolerant High Potential Ethanol Producing Yeast from Agro-Industrial Waste

Fakruddin¹

2013

AJBIO

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“…77.7%, 72.8%, 79.5% (by K. marxianus) and 72.0%, 80.7%, 75.7% (by S. cerevisiae), respectively. Somda et al [79] reported the production of ethanol from mango peel by SSF from two isolates W6 (isolated from wine) and B1 (isolated from Bakers yeast) with the ethanol concentration of 13.0 and 10.0 g l À 1 , respectively after 120 h. Boluda-Aguilar and Lopez-Gomez [80] reported to produce more than 60 l ethanol/tones of fresh lemon peel biomass using SSF process after the application of steam explosion and enzymatic hydrolysis. Jiang et al [81] evaluated cellulose fermentation using cocultivation of cellulolytic C. thermocellum LQRI and saccharolytic T. pseudethanolicus strain X514 in the semicontinuous fermentor with an initial cellulose concentration of 80 g l À 1 and pH controlled between 6.5 and 6.8.…”

Section: Integrated Processes For Ethanol Productionmentioning

confidence: 97%

Bioprospecting thermophilic/thermotolerant microbes for production of lignocellulosic ethanol: A future perspective

Arora

Behera

Kumar

2015

Renewable and Sustainable Energy Reviews

103

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“…In common, glucose is fermented into several end‐products such as bioethanol and energy sources as shown in Equation at a highest conversion rate by S. cerevisiae than all other yeast existing in nature. Furthermore, S. cerevisiae is the most thoroughly investigated microorganism as the beginning of biotechnology era due to its tendency to tolerate and survive in ethanol‐rich solution as well as in the presence of other inhibitory compounds

C_{6} H_{12} O_{6} \to 2 {CH}_{3} {CH}_{2} OH + 2 {CO}_{2} + Energy

…”

Section: Prospective Of Secondary Bioethanol Productionmentioning

confidence: 99%

Evolution toward the utilization of mango leaves as lignocellulosic material in bioethanol production: A review of process parameter and integrated technologies

Tarrsini

Teoh

Shuit

et al. 2019

Env Prog and Sustain Energy

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Recently, the production of bioethanol is shifted to secondary bioethanol which is produced from nonedible lignocellulosic feedstock to avoid the food versus fuel issue. Mango leaves, a kind of nonedible lignocellulosic material (LCM) that possess a relatively 80.7% of holocellulose (inclusive of cellulose and hemicellulose), appear to be potential candidate to serve as cheap substrate source for bioethanol production. Hence, the objective of this article is to present the current scenario and the potential of mango leaves as a substrate source for bioethanol production. This article also provides an overview on various process parameters such as temperature, pH, substrate concentration, and incubation time that required to be optimized for an efficient fermentation process in the bioethanol production from LCM. Apart from that, several integrated fermentation technologies in bioethanol production which include separate hydrolysis and fermentation, simultaneous saccharification and fermentation, simultaneous saccharification and co‐fermentation, and consolidated bioprocessing will also be discussed in this article. Based on the findings, it is clear that mango leaves have the potential to serve as feedstock for bioethanol production.

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Thermotolerant and Alcohol-Tolerant Yeasts Targeted to Optimize Hydrolyzation from Mango Peel for High Bioethanol Production

Cited by 23 publications

References 8 publications

Characterization of Stress Tolerant High Potential Ethanol Producing Yeast from Agro-Industrial Waste

Characterization of Stress Tolerant High Potential Ethanol Producing Yeast from Agro-Industrial Waste

Bioprospecting thermophilic/thermotolerant microbes for production of lignocellulosic ethanol: A future perspective

Evolution toward the utilization of mango leaves as lignocellulosic material in bioethanol production: A review of process parameter and integrated technologies

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