Thermotolerant and Thermophilic Solvent-Utilizing Methylotrophic, Aerobic Bacteria

Al-Awadhi, N.; Egli, Thomas; Hamer, G.; Wehrli, E.

doi:10.1016/s0723-2020(89)80063-3

Cited by 29 publications

(16 citation statements)

References 16 publications

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“…In addition, Matteau and Ramsay (1997) reported toluene biofiltration at 48-508C using an active compost of maple leaves and alfalfa as support materials. Additionally, some studies reported the degradation of BTEX compounds (Chen and Taylor, 1995), and alcohols (Al-Awadhi et al, 1989) by thermophilic microorganisms, as well as the co-treatment of methanol and a-pinene in biotrickling filters maintained at temperatures between 40 and 708C (Allen et al, 2000), and the treatment of ethanol and ammonia at 658C (Heslinga and Van Groenestijn, 1997).…”

Section: Introductionmentioning

confidence: 99%

Mesophilic and thermophilic biotreatment of BTEX‐polluted air in reactors

Mohammad

Veiga

Kennes

2007

Biotech & Bioengineering

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This study compares the removal of a mixture of benzene, toluene, ethylbenzene, and all three xylene isomers (BTEX) in mesophilic and thermophilic (50 degrees C) bioreactors. In the mesophilic reactor fungi became dominant after long-term operation, while bacteria dominated in the thermophilic unit. Microbial acclimation was achieved by exposing the biofilters to initial BTEX loads of 2-15 g m(-3) h(-1), at an empty bed residence time of 96 s. After adaptation, the elimination capacities ranged from 3 to 188 g m(-3) h(-1), depending on the inlet load, for the mesophilic biofilter with removal efficiencies reaching 96%. On the other hand, in the thermophilic reactor the average removal efficiency was 83% with a maximum elimination capacity of 218 g m(-3) h(-1). There was a clear positive relationship between temperature gradients as well as CO(2) production and elimination capacities across the biofilters. The gas phase was sampled at different depths along the reactors observing that the percentage pollutant removal in each section was strongly dependant on the load applied. The fate of individual alkylbenzene compounds was checked, showing the unusually high biodegradation rate of benzene at high loads under thermophilic conditions (100%) compared to its very low removal in the mesophilic reactor at such load (<10%). Such difference was less pronounced for the other pollutants. After 210 days of operation, the dry biomass content for the mesophilic and thermophilic reactors were 0.300 and 0.114 g g(-1) (support), respectively, reaching higher removals under thermophilic conditions with a lower biomass accumulation, that is, lower pressure drop.

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Section: Introductionmentioning

confidence: 99%

Mesophilic and thermophilic biotreatment of BTEX‐polluted air in reactors

Mohammad

Veiga

Kennes

2007

Biotech & Bioengineering

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“…Methylotrophs, microorganisms that use reduced onecarbon compounds, are diverse and ubiquitous (3,20). Even though many methylotrophs have been described, only a few are gram positive (1,2,14,21,30). The industrial advantages of using methanol as a substrate for large-scale production of fermentation products have been outlined by Lentkin and Niekus (29).…”

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confidence: 99%

“…In addition, methanol is available in pure form, it is highly soluble in water, methanol solutions are not explosive, and residual methanol can be easily removed from products after fermentation is completed (29). Bacillus species have been used extensively in industrial fermentation processes, but little information has been published on the isolation of Bacillus species capable of rapid growth on methanol at temperatures above 50°C (2,6,14).…”

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confidence: 99%

L-lysine production at 50 degrees C by mutants of a newly isolated and characterized methylotrophic Bacillus sp

Schendel

Bremmon

Flickinger

et al. 1990

Appl Environ Microbiol

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The amino acid L-lysine was produced from homoserine auxotrophic and S-(2-aminoethyl)-L-cysteineresistant mutants of a newly isolated gram-positive methylotrophic bacterium, capable of growth on methanol at 60°C. The temperature optimum for growth was between 50 and 53°C. These aerobic, gram-positive, endospore-forming, rod-shaped bacteria required biotin and vitamin B12 for growth. Extracts of the bacteria grown on methanol lacked hydroxypyruvate reductase and contained hexulose 6-phosphate synthase activity. Therefore, these bacteria were considered to be type I methylotrophic bacteria of the genus Bacillus. Fed-batch fermentations resulted in cell densities of 50 g of cell dry weight per liter. Biomass yields on carbon, nitrogen, phosphate, and sulfate were determined. Generation of homoserine auxotrophic and amino acid analogresistant mutants resulted in L-lysine concentrations of nearly 20 g/liter in fed-batch fermentations.

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“…The thermotolerant methanol‐utilizing B. brevis S1 (NCIMB12524) isolated by Al‐Awadhi et al[6] was used as the source of genomic DNA for the cloning procedure. The proposed name for this strain is Bacillus methanolicus [7].…”

Section: Methodsmentioning

confidence: 99%

The ribulose monophosphate pathway operon encoding formaldehyde fixation in a thermotolerant methylotroph,Bacillus brevisS1

Yurimoto

Hirai

Yasueda

et al. 2002

FEMS Microbiology Letters

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The hps and phi genes encoding 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase, the key enzymes of the ribulose monophosphate (RuMP) pathway for formaldehyde fixation, were cloned from the chromosomal DNA of a thermotolerant methylotroph, Bacillus brevis S1. Enzyme induction and Northern blot analyses revealed that both the hps and phi genes are induced by methanol or ethanol, and that their expression is controlled polycistronically at the transcription stage. Sequence analysis also suggested that the hps and phi genes constitute an RuMP operon. The gene organization of the RuMP operon and its surrounding region are unique among bacteria possessing the RuMP pathway genes.

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Thermotolerant and Thermophilic Solvent-Utilizing Methylotrophic, Aerobic Bacteria

Cited by 29 publications

References 16 publications

Mesophilic and thermophilic biotreatment of BTEX‐polluted air in reactors

Mesophilic and thermophilic biotreatment of BTEX‐polluted air in reactors

L-lysine production at 50 degrees C by mutants of a newly isolated and characterized methylotrophic Bacillus sp

The ribulose monophosphate pathway operon encoding formaldehyde fixation in a thermotolerant methylotroph,Bacillus brevisS1

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