Thermus arciformis sp. nov., a thermophilic species from a geothermal area

Abstract

Two aerobic, Gram-negative, non-motile, non-sporulating, yellow-pigmented bacteria, strains TH92 T and TH91, were isolated from a hot spring located in Laibin, Guangxi, in the south-eastern geothermal area of China. The isolates grew at 40-77 6C (optimally at 70 6C) and at pH 6.0-9.5 (optimally at pH 7.5-8.0). Phylogenetic analysis of 16S rRNA gene sequences and levels of DNA-DNA relatedness together indicated that the new isolates represented a novel species of the genus Thermus with closest affinity to Thermus aquaticus, Thermus igniterrae and Thermus thermophilus. Compared with their closest relatives, strains TH92 T and TH91 were able to assimilate a wider range of carbohydrates, amino acids and organic acids as sole carbon sources for growth, such as lactose and melibiose. The new isolates had lower combined levels of C 16 : 0 and iso-C 16 : 0 compared with their closest relatives. On the basis of polyphasic taxonomic characterization, strains TH92 T and TH91 are considered to represent a single novel species of the genus Thermus, for which the name Thermus arciformis sp. nov. is proposed. The type strain is TH92 T (5CGMCC 1.6992 T 5JCM 15153 T ).

Exploration of Deinococcus‐Thermus molecular diversity by novel group‐specific PCR primers

“…; Zhang et al. ). As most Deinococcus species are mesophilic or moderately thermophilic, the absence of these bacteria from springs 5 and 7 could be explained by the elevated temperatures recorded at these sites (73°C and 70°C, respectively).…”

“…; Zhang et al. ; Vajna et al. ), while Deinococcus strains exhibit resistance to extreme ionizing and ultraviolet radiations, desiccation, and other DNA damaging conditions (Rainey et al.…”

Parvularcula flava sp. nov., an alphaproteobacterium isolated from surface seawater of the South China Sea

“…Formula of the saline solution was in accordant with that of the inorganic salt of the medium described above in the NaCl reliance analysis, in which 24 % (w/v) NaCl was added. Sensitivity to antibiotics was checked by a twolayer plate method as previously performed (Zhang et al, 2010) except that the modified marine 2216 agar was used. Detailed results of all physiological and biochemical characteristics are summarized in Table S1 and the species description.…”

Thermus anatoliensis sp. nov., a thermophilic bacterium from geothermal waters of Buharkent, Turkey

“…aData for DNA base composition for T. islandicus and T. arciformis were taken from Bjornsdottir et al , Zhang et al , and Vajna et al .…”