Thermus oshimai sp. nov., Isolated from Hot Springs in Portugal, Iceland, and the Azores, and Comment on the Concept of a Limited Geographical Distribution of Thermus Species

Williams, Ruth; Smith, Kelvin E.; Welch, S.G.; Micallef, Janice

doi:10.1099/00207713-46-2-403

Cited by 58 publications

(26 citation statements)

References 21 publications

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“…Thermus-like sequences were the second most prevalent type retrieved from geothermal water (21%) and the 1-month (June 2004) biofilm sample (34%). All biofilm-derived sequence types were most similar to Thermus aquaticus YT-1, isolated from a hot spring (70°C) in Yellowstone's Upper Geyser Basin, a high temperature in the 50 to 70°C range reported for this group (46,57). Because the temperatures at the submerged portion of the rods (60 to 70°C) overlapped well with this range, we were not surprised that Thermus-like organisms, given retrieval from geothermal water, were present in early biofilms and maintained representation through 8 months (February 2006).…”

Section: Roseiflexus Strain Rs-1 (Unpublished)mentioning

confidence: 88%

“…Because the temperatures at the submerged portion of the rods (60 to 70°C) overlapped well with this range, we were not surprised that Thermus-like organisms, given retrieval from geothermal water, were present in early biofilms and maintained representation through 8 months (February 2006). Given that Thermus isolates grow chemoheterotrophically (46,57) and Thermocrinis isolates grow chemolithotrophically (13), it seems likely that Thermus isolates would be more selected in the context of a complex community biofilm with fixed carbon sources.…”

Section: Roseiflexus Strain Rs-1 (Unpublished)mentioning

confidence: 99%

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Formation of Multilayered Photosynthetic Biofilms in an Alkaline Thermal Spring in Yellowstone National Park, Wyoming

Boomer

Noll²,

Geesey

et al. 2009

Appl Environ Microbiol

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In this study, glass rods suspended at the air-water interface in the runoff channel of Fairy Geyser, Yellowstone National Park, WY, were used as a substratum to promote the development of biofilms that resembled multilayered mat communities in the splash zone at the geyser's source. This approach enabled the establishment of the temporal relationship between the appearance of Cyanobacteria, which ultimately formed the outer green layer, and the development of a red underlayer containing Roseiflexus-like Chloroflexi. This is the first study to define time-dependent successional events involved in the development of differently colored layers within microbial mats associated with many thermal features in Yellowstone National Park. Initial (1-month) biofilms were localized below the air-water interface (60 to 70°C), and the majority of retrieved bacterial sequence types were similar to Synechococcus and Thermus isolates. Biofilms then shifted, becoming established at and above the air-water interface after 3 months. During winter sampling (6 to 8 months), distinct reddish orange microcolonies were observed, consistent with the appearance of Roseiflexus-like sequences and bacteriochlorophyll a pigment signatures. Additionally, populations of Cyanobacteria diversified to include both unicellular and filamentous cell and sequence types. Distinct green and red layers were observed at 13 months. Planctomycetes-like sequences were also retrieved in high abundance from final biofilm layers and winter samples. Finally, biomass associated with geyser vent water contained Roseiflexus-like sequence types, in addition to other high-abundance sequence types retrieved from biofilm samples, supporting the idea that geothermal water serves as an inoculum for these habitats.

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Section: Roseiflexus Strain Rs-1 (Unpublished)mentioning

confidence: 88%

Section: Roseiflexus Strain Rs-1 (Unpublished)mentioning

confidence: 99%

Formation of Multilayered Photosynthetic Biofilms in an Alkaline Thermal Spring in Yellowstone National Park, Wyoming

Boomer

Noll²,

Geesey

et al. 2009

Appl Environ Microbiol

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“…On the basis of the phylogenetic studies of 16S rRNA, growth temperature, polar lipid pattern and the hydroxy fatty acid composition, eight species of the genus Thermus have been validly described. These are Thermus aquaticus (BROCK and FREEZE 1969), T. thermophilus (OSHIMA andIMAHORI 1974, WILLIAMS et al 1995), T. filiformis (HUDSON 1987), T. scotoductus (KRISTJANSSON et al 1994), T. brockianus (WILLIAMS et al 1995), T. oshimai (WILLIAMS et al 1996), T. igniterrae and T. antranikianni (CHUNG et al 2000). Rehai of Tengchong is only one volcano geothermic region in China.…”

mentioning

confidence: 99%

Thermus rehai sp. nov., isolated from Rehai of Tengchong, Yunnan Province, China

Lü

Chen

Peng

et al. 2002

J. Basic Microbiol.

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A yellow‐pigmented strain of the genus Thermus, with optimum growth temperatures about 65 ∼ 70 °C, was isolated from the hot springs in Rehai of Tengchong, Yunnan Province, China. Morphological, physiological and biochemical characteristics, pigment analysis of RH99‐GF7504 strain and its phylogenetic analysis of 16S rDNA showed that this organism represented a new species of the genus Thermus. This strain had maximum temperatures for growth below 80 °C. The new isolate from Rehai of Tengchong could be distinguished from other strains of the genus Thermus by its special structure and by its inability to hydrolyze gelatin and starch. On the basis of phylogenetic analysis, morphological, physiological and biochemical characteristics, the name Thermus rehai sp nov is proposed for the species, represented by strain RH99‐GF7504 (CCTCC‐AB200292).

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“…However, as LNE-8 clusters within the Thermus scotoductus cluster, the results suggest that LNE-8 may represent a new subspecies of T. scotoductus (Kristjansson et al 1994;Skirnisdottir et al 2001). LNE-2 was showing very high homology to Thermus oshimai, which has been isolated from the same geographical hot spring location in Iceland but also in the Azores (Williams et al 1996 Green nonsulfur bacterium clone OPB34 (AF027044) Figure 1. Evolutionary maximum-likelihood phylogenetic dendrogram of the 16S rRNA sequences detected in the sulphide-rich hot spring biomass (designated SRI) and by low nutrient enrichments (designated LNE).…”

Section: Specific Amplification Of 16s Rrna Genes and Phylogenetic Anmentioning

confidence: 88%

Use of low nutrient enrichments to access novel amylase genes in silent diversity of thermophiles

Hobel¹,

Marteinsson²,

Hauksdóttir³

et al. 2004

World J Microbiol Biotechnol

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By combining low nutrient enrichments and molecular methods, a high diversity of new amylase genes was detected in a neutral sulphide-rich hot spring in Iceland. Enrichments based on hot spring water and low concentrations of starch were used to select slow-growing, starch-degrading microorganisms. Six enrichments had in total 17 bacterial types detected by 16S rRNA analysis, mostly related to the Thermus-Deinococcus group, green non-sulphur bacteria, gram positives, and uncultivated new candidate divisions. No Archaea were found. The apparent 16S rRNA species composition of the enrichments was very different from that of the microbial mat in the same hot spring. DNA samples obtained from 4 enrichments and from hot spring biomass were screened by PCR for amylase genes in glycoside-hydrolase family 13. Degenerate primers, based on conserved amino acid sequences from multiple alignments of family 13, enabled the detection of 18 amylase sequence types in the enrichments, including a-amylases, a-glucosidases, 1,4-a-glucan branching enzymes, cyclomaltodextrin hydrolases, maltogenic amylases and neopullulanases, and unspecified family 13 glycoside-hydrolases. Only one unique neopullulanase sequence, also found in most of the enrichments, was detected in the hot spring biomass DNA. The results suggest that the enrichment method combined with sequence-based screening is an efficient way to access the silent, i.e. not detectable, gene diversity in natural environments.

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Thermus oshimai sp. nov., Isolated from Hot Springs in Portugal, Iceland, and the Azores, and Comment on the Concept of a Limited Geographical Distribution of Thermus Species

Cited by 58 publications

References 21 publications

Formation of Multilayered Photosynthetic Biofilms in an Alkaline Thermal Spring in Yellowstone National Park, Wyoming

Formation of Multilayered Photosynthetic Biofilms in an Alkaline Thermal Spring in Yellowstone National Park, Wyoming

Thermus rehai sp. nov., isolated from Rehai of Tengchong, Yunnan Province, China

Use of low nutrient enrichments to access novel amylase genes in silent diversity of thermophiles

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