Thiamine Supplementation Alleviates Lipopolysaccharide-Triggered Adaptive Inflammatory Response and Modulates Energy State via Suppression of NFκB/p38 MAPK/AMPK Signaling in Rumen Epithelial Cells of Goats

Ma, Yuxiang; Elmhadi, Mawda; Wang, Chao; Li, Zelin; Zhang, Hao; He, Banglin; Zhao, Xiujuan; Zhang, Zhenbin; Wang, Hongrong

doi:10.3390/antiox11102048

Cited by 11 publications

(5 citation statements)

References 59 publications

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“…As the activity of MAPKs increases, especially p38 MAPK, inflammatory mediators are also enhanced at the transcriptional and translational levels to enhance the inflammatory response [ 54 ]. It is well documented that stimulation of RECs for 6 h using LPS (10 µg/mL) can activate the MAPK pathway [ 43 ], consistent with our results, while GRECs (goat rumen epithelial cells) required LPS (5 µg/mL) stimulation for 6 h at a lower dose to activate [ 55 ]. The results of the experiment indicate the activation of the NF-κB and MAPK signaling pathways through p65 translocation into the nucleus following LPS stimulation.…”

Section: Discussionsupporting

confidence: 91%

Sodium Propionate Relieves LPS-Induced Inflammation by Suppressing the NF-ĸB and MAPK Signaling Pathways in Rumen Epithelial Cells of Holstein Cows

Zhao

Wei

et al. 2023

Toxins

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Subacute ruminal acidosis (SARA) is a prevalent disease in intensive dairy farming, and the rumen environment of diseased cows acidifies, leading to the rupture of gram-negative bacteria to release lipopolysaccharide (LPS). LPS can cause rumentitis and other complications, such as liver abscess, mastitis and laminitis. Propionate, commonly used in the dairy industry as a feed additive, has anti-inflammatory effects, but its mechanism is unclear. This study aims to investigate whether sodium propionate (SP) reduces LPS-induced inflammation in rumen epithelial cells (RECs) and the underlying mechanism. RECs were stimulated with different time (0, 1, 3, 6, 9, 18 h) and different concentrations of LPS (0, 1, 5, 10 μg/mL) to establish an inflammation model. Then, RECs were treated with SP (15, 25, 35 mM) or 10 μM PDTC in advance and stimulated by LPS for the assessment. The results showed that LPS (6h and 10 μg/mL) could stimulate the phosphorylation of NF-κB p65, IκB, JNK, ERK and p38 MAPK through TLR4, and increase the release of TNF-α, IL-1β and IL-6. SP (35 mM) can reduce the expression of cytokines by effectively inhibiting the NF-κB and MAPK inflammatory pathways. This study confirmed that SP inhibited LPS-induced inflammatory responses through NF-κB and MAPK in RECs, providing potential therapeutic targets and drugs for the prevention and treatment of SARA.

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Section: Discussionsupporting

confidence: 91%

Sodium Propionate Relieves LPS-Induced Inflammation by Suppressing the NF-ĸB and MAPK Signaling Pathways in Rumen Epithelial Cells of Holstein Cows

Zhao

Wei

et al. 2023

Toxins

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“…25 More, the modulation of thiamine metabolism inhibits the development of inflammation in a variety of diseases. 26,27 These findings provide convincing evidence for the effectiveness of WZYD in the treatment of CNAG in the clinical setting by suppressing inflammation and modulating core metabolism process. To confirm the effect of WZYD on inflammatory factors in patients with CNAG, patient serum was examined, and ELISA results demonstrated that WZYD treatment effectively reduced serum levels of TNF-α, IL-10, and COX-2.…”

Section: Discussionmentioning

confidence: 66%

Metabolomics Profiles Reveal the Efficacy of Wuzhuyu Decoction on Patients with Chronic Non-Atrophic Gastritis

Hu,

Zeng,

Zhang

et al. 2023

DDDT

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Chronic non-atrophic gastritis (CNAG) is a common clinical gastrointestinal disease with a long and recurrent course. In China, Wuzhuyu decoction (WZYD) has been used for centuries to treat gastrointestinal disorders. To unravel the efficacy and mechanism of WZYD for CNAG, a clinical study was conducted. And metabolomics was used to explore the mechanism of WZYD for CNAG patients. Methods: Twenty patients in total were recruited in this study (Nos. ChiCTR2200062296) and the protocol was approved by the Ethics Committee (Approval number: KY-2022-2-6-1) and complied with the Declaration of Helsinki. The formula granule of WZYD were assessed by UHPLC-QQQ-TOF to discern the main potential active compounds. The endoscopy evaluation and histopathological changes were detected as effective indicators. Serum samples from patients were used for metabolomics. Inflammatory factors in patients' serum were determined by ELISA. Metabolomics revealed a series of differential metabolites and signaling pathways. Results: WZYD was capable to prevent CNAG by ameliorating score of endoscopy evaluation including erosion, hemorrhage, as well as chronic inflammation and active chronic inflammation score after treatment were decreased. The results indicated that 10 core metabolic components were associated with the treatment of WZYD. Moreover, these metabolic components proved that pyrimidine metabolism and thiamine metabolism were critically responsible for CNAG. In addition, WZYD treatment effectively reduced serum levels of TNF-α, IL-10, and COX-2. Conclusion: Altogether, WZYD can effectively alleviate CNAG by inhibiting inflammation and regulating related metabolic processes, which might be the molecular mechanism of WZYD treatment of CNAG. More studies are warranted to be conducted in this area. Trial Registration: ChiCTR, ChiCTR2200062296. Registered 1 August 2022, https://www.chictr.org.cn/com/25/showprojen.aspx? proj=174027.

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“…A high concentrate diet can upregulate the expression of IL-1β, IL-2, IL-22, and other in ammatory factors [52]. In vitro studies have shown that LPS can signi cantly increase the level of IL-6 and the phosphorylation of NF-κB/P38 and MAPKs in rumen epithelial cells [53]. These results suggest that the LPS-induced overactivation of NF-κB and MAPKs mediates rumen in ammation during subacute acidosis.…”

Section: Discussionmentioning

confidence: 88%

Molecular mechanism of ruminal epithelial inflammatory damage in sheep with subacute acidosis

Yan,

Duan,

et al. 2023

Preprint

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Background The long-term feeding of high-concentrate diets to ruminants will damage the structure and function of their rumen flora, leading to changes in the gastrointestinal patterns of digestive nutrients and metabolic factors, and causing subacute rumen acidosis (SARA). Methods 28 small-tailed Han sheep were randomly selected and divided into three groups, namely the control group, SARA model group, and treatment group. The control group was fed low concentrate fodder, the model group was fed high concentrate fodder, and the treatment group was fed the HC first and then the LC after successfully establishing the model (n = 9). Results SARA-model sheep had high concentrations of lipopolysaccharide (LPS) in their rumen fluid and blood, whereas tumor necrosis factor-α (TNF-α) was significantly elevated in the rumen fluid, with no difference in the blood. The levels of inflammation-related proteins, namely cyclooxygenase-2 (COX-2), interleukin-6 (IL-6), TNF-α, and Toll-like receptor-4 (TLR-4), were significantly increased in the rumen epithelium of SARA-model sheep. Phosphorylation levels of nuclear transcription factor-κB (NF-κB) and mitogen-activated protein kinases (MAPKs) in the SARA group were significantly higher than those in the control and treatment groups. The phosphorylation levels of NF-κB and MAPKs inflammatory mediators and protein levels of inflammatory factors in the SARA-model sheep rumen abdominal sac were significantly higher than those in the rumen dorsal sac. The expression of tight junction proteins ZO-1, occludin, claudin-1 and claudin-4 decreased compared with that in the control group. The expression of light chain 3 (LC-3) increased in rumen epithelium of SARA sheep, while the trend of autophagy substrate sequestosome-1 (P62) was opposite to that of LC-3. Conclusions These results indicate that SARA leads to a high concentration of ruminal LPS, which significantly increases the expression and synthesis of pro-inflammatory cytokines in the rumen epithelium, through the over-activation of NF-κB and MAPK inflammatory pathways, thereby inducing rumenitis, damaging the integrity of rumen epithelium; moreover, damage to the rumen abdominal sac is more serious than that to the rumen dorsal sac. In the process of rumen gastritis, autophagy is involved in the regulation and inhibition of the inflammatory response.

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Thiamine Supplementation Alleviates Lipopolysaccharide-Triggered Adaptive Inflammatory Response and Modulates Energy State via Suppression of NFκB/p38 MAPK/AMPK Signaling in Rumen Epithelial Cells of Goats

Cited by 11 publications

References 59 publications

Sodium Propionate Relieves LPS-Induced Inflammation by Suppressing the NF-ĸB and MAPK Signaling Pathways in Rumen Epithelial Cells of Holstein Cows

Sodium Propionate Relieves LPS-Induced Inflammation by Suppressing the NF-ĸB and MAPK Signaling Pathways in Rumen Epithelial Cells of Holstein Cows

Metabolomics Profiles Reveal the Efficacy of Wuzhuyu Decoction on Patients with Chronic Non-Atrophic Gastritis

Molecular mechanism of ruminal epithelial inflammatory damage in sheep with subacute acidosis

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