Thiazolidinedione suppresses the expression of erythroid phenotype in erythroleukemia cell line K562

Hirase, Nobuhisa; Yanase, Toshihiko; Mu, Yi Ming; Muta, Koichiro; Umemura, Tomonari; Takayanagi, Ryoichi; Nawata, Hajime

doi:10.1016/s0145-2126(99)00200-3

Cited by 25 publications

(14 citation statements)

References 26 publications

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“…These results were reported previously by the same group in Ph-positive lymphocytic leukemia cell lines, where TZD18 promoted cell death and acted synergistically to enhance the effect of Imatinib [179]. Hirase et al tested the effects of TZDs in K562 cells, which have an erythroid nature and the potential to differentiate into megakaryocytes [180]. TZD inhibited both cell proliferation and the erythroid phenotype of K562 cells.…”

Section: Pparγ and Pparγ Ligands As Potential Therapy For Hematolosupporting

confidence: 76%

Role of Peroxisome Proliferator‐Activated Receptor Gamma and Its Ligands in the Treatment of Hematological Malignancies

et al. 2008

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Peroxisome proliferator-activated receptor gamma (PPARγ) is a multifunctional transcription factor with important regulatory roles in inflammation, cellular growth, differentiation, and apoptosis. PPARγ is expressed in a variety of immune cells as well as in numerous leukemias and lymphomas. Here, we review recent studies that provide new insights into the mechanisms by which PPARγ ligands influence hematological malignant cell growth, differentiation, and survival. Understanding the diverse properties of PPARγ ligands is crucial for the development of new therapeutic approaches for hematological malignancies.

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Section: Pparγ and Pparγ Ligands As Potential Therapy For Hematolosupporting

confidence: 76%

Role of Peroxisome Proliferator‐Activated Receptor Gamma and Its Ligands in the Treatment of Hematological Malignancies

et al. 2008

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“…The protocol essentially followed a previously described method (27). Briefly, 3 ϫ 10 5 BMCs and AMCs were incubated with either phycoerythrin (PE)-conjugated antimouse CD11b, CD34, CD44, CD45, c-kit and Sca-1 monoclonal antibodies (BD Biosciences, San Jose, CA), or an isotype-matched PE-conjugated rat IgG (BD Biosciences) for 30 min at 4 C. The cells were finally analyzed on a FACScan flow cytometer (BD Biosciences).…”

Section: Flow Cytometrymentioning

confidence: 99%

“…3A. There were 3 ϫ 10 5 AMCs incubated with either PE-conjugated antimouse CD11b, CD34, CD44, CD45, c-kit, and sca-1 monoclonal antibodies, or an isotype-matched PE-conjugated rat IgG for 30 min at 4 C and finally analyzed on a FACScan flow cytometer as previously described (27). Fluorescence-2 (FL2) is a channel that collects the emitted light between 560 and 585 nm.…”

Section: Amcs Infected With Adx-bsf-1 Were Steroidogenicmentioning

confidence: 99%

Adipose Tissue-Derived and Bone Marrow-Derived Mesenchymal Cells Develop into Different Lineage of Steroidogenic Cells by Forced Expression of Steroidogenic Factor 1

et al. 2008

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Steroidogenic factor 1 (SF-1)/adrenal 4 binding protein is an essential nuclear receptor for steroidogenesis, as well as for adrenal and gonadal gland development. We have previously clarified that adenovirus-mediated forced expression of SF-1 can transform long-term cultured mouse bone marrow mesenchymal cells (BMCs) into ACTH-responsive steroidogenic cells. In the present study, we extended this work to adipose tissue-derived mesenchymal cells (AMCs) and compared its steroidogenic capacity with those of BMCs prepared from the identical mouse. Several cell surface markers, including potential mesenchymal cell markers, were identical in both cell types, and, as expected, forced expression of SF-1 caused AMCs to be transformed into ACTH-responsive steroidogenic cells. However, more elaborate studies revealed that the steroidogenic property of AMCs was rather different from that of BMCs, especially in steroidogenic lineage. In response to increased SF-1 expression and/or treatment with retinoic acid, AMCs were much more prone to produce adrenal steroid, corticosterone rather than gonadal steroid, testosterone, whereas the contrary was evident in BMCs. Such marked differences in steroidogenic profiles between AMCs and BMCs were also evident by the changes of steroidogenic enzymes. These novel results suggest a promising utility of AMCs for autologous cell regeneration therapy for patients with steroid insufficiency and also a necessity for appropriate tissue selection in preparing mesenchymal stem cells according to the aim. The different steroidogenic potency of AMCs or BMCs might provide a good model for the clarification of the mechanism of tissue- or cell-specific adrenal and gonadal steroidogenic cell differentiation.

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“…The protocol essentially followed a previously described method (Hirase et al 2000). Briefly, 1!10 5 BMCs were incubated with either phycoerythin (PE)-conjugated anti-human c-kit, CD11b, CD31, CD34, CD44, CD45, and CD105 monoclonal antibodies (BD Biosciences, Franklin Lakes, NJ, USA) or an isotype-matched PE-conjugated mouse IgG (BD Biosciences) for 30 min at 4 8C.…”

Section: Flowcytometrymentioning

confidence: 99%

Steroidogenic factor 1/adrenal 4 binding protein transforms human bone marrow mesenchymal cells into steroidogenic cells

Tanaka¹,

Gondo²,

Okabe³

et al. 2007

Journal of Molecular Endocrinology

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Steroidogenic factor 1/adrenal 4 binding protein (SF-1/Ad4BP) is an essential nuclear receptor for steroidogenesis as well as for adrenal and gonadal gland development. Mesenchymal bone marrow cells (BMCs) contain pluripotent progenitor cells, which differentiate into multiple lineages. In a previous study, we reported that adenovirus-mediated forced expression of SF-1 could transform mouse primary long-term cultured BMCs into steroidogenic cells. For future clinical application, trials using human BMCs would be indispensable. In this study, we examined whether SF-1 could transform human BMCs into steroidogenic cells and compared the steroid profile of these cellswith that of mouse steroidogenic BMCs. Primary cultured human BMCs infected with adenovirus containing bovine SF-1 cDNA could produce progesterone, corticosterone, cortisol, dehydroepiandrosterone, testosterone, and estradiol. Such a mixed character of adrenal and gonadal steroid production in human BMCs was supported by the expressions of P450scc, 3b-hydroxysteroid dehydrogenase (3b-HSD), P450c21, P450c11, P450c17, 17b-HSD, and P450arom mRNAs. Unlike mouse steroidogenic BMCs, introduction of SF-1 into human BMCs caused dramatic inductions of both ACTH and LH receptors, thus leading to good responsiveness of the cells to ACTH and LH respectively. Importantly, among several factors that are known to be closely associated with adrenal and/or gonadal development, introduction of only SF-1 enabled the human BMCs to express P450scc and to produce cortisol and testosterone, suggesting that SF-1 is truly a master regulator for the production of steroidogenic cells from human BMCs.

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Thiazolidinedione suppresses the expression of erythroid phenotype in erythroleukemia cell line K562

Cited by 25 publications

References 26 publications

Role of Peroxisome Proliferator‐Activated Receptor Gamma and Its Ligands in the Treatment of Hematological Malignancies

Role of Peroxisome Proliferator‐Activated Receptor Gamma and Its Ligands in the Treatment of Hematological Malignancies

Adipose Tissue-Derived and Bone Marrow-Derived Mesenchymal Cells Develop into Different Lineage of Steroidogenic Cells by Forced Expression of Steroidogenic Factor 1

Steroidogenic factor 1/adrenal 4 binding protein transforms human bone marrow mesenchymal cells into steroidogenic cells

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