2015
DOI: 10.1016/j.anireprosci.2015.08.003
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Thiazovivin, a Rho kinase inhibitor, improves stemness maintenance of embryo-derived stem-like cells under chemically defined culture conditions in cattle

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Cited by 23 publications
(13 citation statements)
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“…ROCK1 regulates actin-myosin contraction (McBeath et al, 2004), facilitates expansion of PSCs (Ohgushi et al, 2015;Park et al, 2015), and acute inhibition by small molecules leads to a "relaxed" cell phenotype with decreased stiffness (Kinney et al, 2014;Lee et al, 2006). However, we found that prolonged silencing of ROCK1 in hiPSCs (6 days) resulted in cells that were actually two-fold stiffer than either the CDH1 KD cells or the control CRISPRi cells.…”
Section: Discussionmentioning
confidence: 66%
See 1 more Smart Citation
“…ROCK1 regulates actin-myosin contraction (McBeath et al, 2004), facilitates expansion of PSCs (Ohgushi et al, 2015;Park et al, 2015), and acute inhibition by small molecules leads to a "relaxed" cell phenotype with decreased stiffness (Kinney et al, 2014;Lee et al, 2006). However, we found that prolonged silencing of ROCK1 in hiPSCs (6 days) resulted in cells that were actually two-fold stiffer than either the CDH1 KD cells or the control CRISPRi cells.…”
Section: Discussionmentioning
confidence: 66%
“…Accutase (STEMCELL Technologies) was used to dissociate hiPSCs to single cells during passaging. Cells were passaged at a seeding density of 12,000 cells per cm 2 and the small molecule Rho-associated coiled-coil kinase (ROCK) inhibitor, Y-276932 (10µM; Selleckchem) was added to the media upon passaging to promote survival (Park et al, 2015;Watanabe et al, 2007).…”
Section: Human Ipsc Line Generation and Culturementioning
confidence: 99%
“…IVP of bovine fertilized embryos was conducted as previously described [15]. The thawed HanWoo semen (purchased from HanWoo improvement center, Seosan, Korea) was deposited on the top of a discontinuous Percoll gradient prepared by depositing 2 ml of 90% Percoll under 2 ml of 45% Percoll in a 15 ml centrifuge tube, and the sample was then centrifuged for 20 min at 252 x g .…”
Section: Methodsmentioning
confidence: 99%
“…There have been several attempts to generate authentic bESCs using small molecules. Recently, embryo-derived stem-like cells (eSLCs) from bovine embryos were successfully generated using three inhibitors (3i): PD18435 (MEK inhibitor), CHIR99021, and SU5402, a fibroblast growth factor receptor (FGFR) inhibitor [14, 15]. The eSLCs in 3i were able to proliferate and be maintained in culture for over 50 passages with the normal karyotype.…”
Section: Introductionmentioning
confidence: 99%
“…Recent studies have reported that ROCK inhibitors, specifically Thiazovivin and Y-27632, can effectively maintain the pluripotency and increase the viability of stem cells after freezing and thawing 9 , 10 . In cows, several studies have shown that these small molecules are also able to establish embryo-derived stem cells 11 13 . However, little is known about the relationship between small molecules and the resistance to heat stress during short-term embryo holding at a wide temperature range.…”
Section: Introductionmentioning
confidence: 99%