Thicker three-dimensional tissue from a “symbiotic recycling system” combining mammalian cells and algae

Haraguchi, Yuji; Kagawa, Y.; Sakaguchi, Katsuhisa; Matsuura, Kiyotaka; Shimizu, Tatsuya; Okano, Teruo

doi:10.1038/srep41594

Cited by 52 publications

(49 citation statements)

References 43 publications

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“…S. subsalsa and A. platensis are freshwater‐derived and salt lake‐derived Cyanophyta, respectively. All microalgae were cultured in a free‐floating culture flask (AGC Techno Glass Co., Ltd., Shizuoka, Japan) under continuous light (approximately 500–700 lx) at room temperature (25°C) . Phase‐contrast images of microalgal cultures were obtained using a microscope (ELIPSE TS2, Nikon, Tokyo, Japan) with a software (NIS‐Elements BR, Nikon).…”

Section: Methodsmentioning

confidence: 99%

Mammalian cell cultivation using nutrients extracted from microalgae

Okamoto

Haraguchi

Sawamura

et al. 2019

Biotechnology Progress

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Mammalian cells have been used in various research fields. More recently, cultured cells have been used as the cell source of “cultured meat.” Cell cultivation requires media containing nutrients, of which glucose and amino acids are the essential ones. These nutrients are generally derived from grains or heterotrophic microorganisms, which also require various nutrients derived from grains. Grain culture, in turn, requires many chemical fertilizers and agrochemicals, which can cause greenhouse gas emission and environmental contamination. Furthermore, grain production is greatly influenced by environmental changes. In contrast, microalgae efficiently synthesize various nutrients using solar energy, water, and inorganic substances, which are widely used in the energy sector. In this study, we aimed to apply nutrients extracted from microalgae in the culture media for mammalian cell cultivation. Glucose was efficiently extracted from Chlorococcum littorale or Arthrospira platensis using sulfuric acid, whereas 18 of the 20 proteinogenic amino acids were efficiently extracted from Chlorella vulgaris using hydrochloric acid. We further investigated whether nutrients present in the algal extracts could be used in mammalian cell cultivation. Although almost all C2C12 mouse myoblasts died during cultivation in a glucose‐ and amino acid‐free medium, the cell death was rescued by adding algal extract(s) into the nutrient‐deficient media. This indicates that nutrients present in algal extracts can be used for mammalian cell cultivation. This study is the first step toward the establishment of a new cell culture system that can reduce environmental loads and remain unaffected by the impact of environmental changes.

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Section: Methodsmentioning

confidence: 99%

Mammalian cell cultivation using nutrients extracted from microalgae

Okamoto

Haraguchi

Sawamura

et al. 2019

Biotechnology Progress

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“…The cell sheets maintain cell‐to‐cell junctions and extracellular matrix (ECM) and can be layered on temperature‐responsive culture dishes or normal polystyrene culture dishes for fabricating thicker tissues. Cell sheet technology has been applied to regenerating various injured tissues . Cell sheet‐tissues have been used in various clinical trials for damaged tissue regeneration …”

Section: Introductionmentioning

confidence: 99%

Rapid fabrication of detachable three‐dimensional tissues by layering of cell sheets with heating centrifuge

Haraguchi

Kagawa

Hasegawa

et al. 2018

Biotechnology Progress

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Confluent cultured cells on a temperature-responsive culture dish can be harvested as an intact cell sheet by decreasing temperature below 32°C. A three-dimensional (3-D) tissue can be fabricated by the layering of cell sheets. A resulting 3-D multilayered cell sheet-tissue on a temperature-responsive culture dish can be also harvested without any damage by only temperature decreasing. For shortening the fabrication time of the 3-D multilayered constructs, we attempted to layer cell sheets on a temperature-responsive culture dish with centrifugation. However, when a cell sheet was attached to the culture surface with a conventional centrifuge at 22-23°C, the cell sheet hardly adhere to the surface due to its noncell adhesiveness. Therefore, in this study, we have developed a heating centrifuge. In centrifugation (55g) at 36-37°C, the cell sheet adhered tightly within 5 min to the dish without significant cell damage. Additionally, centrifugation accelerated the cell sheet-layering process. The heating centrifugation shortened the fabrication time by one-fifth compared to a multilayer tissue fabrication without centrifugation. Furthermore, the multilayered constructs were finally detached from the dishes by decreasing temperature. This rapid tissue-fabrication method will be used as a valuable tool in the field of tissue engineering and regenerative therapy. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:692-701, 2018.

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“…However, the hypoxic microenvironment created when multiple cell sheets are stacked—in the absence of a vascular network—limits the thickness of viable layered 3D tissue to 40–80 μm ( Shimizu et al, 2006 ), which presents a major obstacle to their development and deployment. To overcome this drawback, in 2017 Haraguchi et al (2017) explored the idea of using C. littorale , a unicellular green spheroidal alga with a high capacity for CO 2 fixation and a significant photosynthetic potential ( Satoh et al, 2002 ). By co-culturing multilayer cell sheets composed of rat cardiomyocytes together with C. littorale , they were able to bioengineer significantly thicker (160 μm) viable tissue consisting of up five cell sheets.…”

Section: Photosynthetic Biomaterials For Tissue Engineering and Regenmentioning

confidence: 99%

Photosymbiosis for Biomedical Applications

Chávez

Moellhoff

Schenck

et al. 2020

Front. Bioeng. Biotechnol.

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Without the sustained provision of adequate levels of oxygen by the cardiovascular system, the tissues of higher animals are incapable of maintaining normal metabolic activity, and hence cannot survive. The consequence of this evolutionarily suboptimal design is that humans are dependent on cardiovascular perfusion, and therefore highly susceptible to alterations in its normal function. However, hope may be at hand. “Photosynthetic strategies,” based on the recognition that photosynthesis is the source of all oxygen, offer a revolutionary and promising solution to pathologies related to tissue hypoxia. These approaches, which have been under development over the past 20 years, seek to harness photosynthetic microorganisms as a local and controllable source of oxygen to circumvent the need for blood perfusion to sustain tissue survival. To date, their applications extend from the in vitro creation of artificial human tissues to the photosynthetic maintenance of oxygen-deprived organs both in vivo and ex vivo , while their potential use in other medical approaches has just begun to be explored. This review provides an overview of the state of the art of photosynthetic technologies and its innovative applications, as well as an expert assessment of the major challenges and how they can be addressed.

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Thicker three-dimensional tissue from a “symbiotic recycling system” combining mammalian cells and algae

Cited by 52 publications

References 43 publications

Mammalian cell cultivation using nutrients extracted from microalgae

Mammalian cell cultivation using nutrients extracted from microalgae

Rapid fabrication of detachable three‐dimensional tissues by layering of cell sheets with heating centrifuge

Photosymbiosis for Biomedical Applications

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