Thickness-controllable electrospun fibers promote tubular structure formation by&amp;nbsp;endothelial progenitor cells

Hong, Jong Kyu; Bang, Ju Yup; Xu, Guan; Lee, Jun Hee; Kim, Yeon Ju; Lee, Ho Jun; Kim, Han Seong; Kwon, Sang Mo

doi:10.2147/ijn.s73096

Cited by 11 publications

(6 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the microvascular epithelial cells showed sparse extracellular matrix on the L-PLA scaffolds with pore size from 38 to 150 lm, while multilayered lining was formed on the scaffolds with pore size \38 lm (Zeltinger et al 2001). It was also shown that the depth of invasion (160 lm after 4 h) of endothelial progenitor cells into electrospun fibrous scaffold and their further colonization was increased with increasing pore size ([45 lm) (Hong et al 2015). Another study showed only 100 lm infiltration of the endothelial progenitors into electrospun fibrous scaffolds with pore size \20 lm after 7 days of culturing (Blakeney et al 2011).…”

Section: Pore Sizes For the Regeneration Of Other Connective Tissuesmentioning

confidence: 98%

Scaffolds and cells for tissue regeneration: different scaffold pore sizes—different cell effects

et al. 2015

View full text Add to dashboard Cite

During the last decade biomaterial sciences and tissue engineering have become new scientific fields supplying rising demand of regenerative therapy. Tissue engineering requires consolidation of a broad knowledge of cell biology and modern biotechnology investigating biocompatibility of materials and their application for the reconstruction of damaged organs and tissues. Stem cell-based tissue regeneration started from the direct cell transplantation into damaged tissues or blood vessels. However, it is difficult to track transplanted cells and keep them in one particular place of diseased organ. Recently, new technologies such as cultivation of stem cell on the scaffolds and subsequently their implantation into injured tissue have been extensively developed. Successful tissue regeneration requires scaffolds with particular mechanical stability or biodegradability, appropriate size, surface roughness and porosity to provide a suitable microenvironment for the sufficient cell-cell interaction, cell migration, proliferation and differentiation. Further functioning of implanted cells highly depends on the scaffold pore sizes that play an essential role in nutrient and oxygen diffusion and waste removal. In addition, pore sizes strongly influence cell adhesion, cell-cell interaction and cell transmigration across the membrane depending on the various purposes of tissue regeneration. Therefore, this review will highlight contemporary tendencies in application of non-degradable scaffolds and stem cells in regenerative medicine with a particular focus on the pore sizes significantly affecting final recover of diseased organs.

show abstract

Section: Pore Sizes For the Regeneration Of Other Connective Tissuesmentioning

confidence: 98%

Scaffolds and cells for tissue regeneration: different scaffold pore sizes—different cell effects

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Cellular tubulogenesis assay is a common tool to mimic many stages of in vivo angiogenesis, including cell migration, proliferation, vessel branching, and anastomosis processes. 49 , 50 For this assay, HUVECs are supported on Matrigel, and the effects of biomaterials or molecules are examined on the tubular networking of cells. The nanosized bioactive glasses that could release silicate ions at 2.87–8.39 µg/mL presented significantly higher number of tubules and more stable tubular structure when compared to the glass-free control group.…”

Section: Effects Of Si Ion Release On Angiogenic Events (In Vitro Andmentioning

confidence: 99%

A mini review focused on the proangiogenic role of silicate ions released from silicon-containing biomaterials

et al. 2017

View full text Add to dashboard Cite

Angiogenesis is considered an important issue in the development of biomaterials for the successful regeneration of tissues including bone. While growth factors are commonly used with biomaterials to promote angiogenesis, some ions released from biomaterials can also contribute to angiogenic events. Many silica-based biomaterials have been widely used for the repair and regeneration of tissues, mainly hard tissues such as bone and tooth structure. They have shown excellent performance in bone formation by stimulating angiogenesis. The release of silicate and others (Co and Cu ions) has therefore been implicated to play critical roles in the angiogenesis process. In this short review, we highlight the in vitro and in vivo findings of angiogenesis (and the related bone formation) stimulated by the various types of silicon-containing biomaterials where silicate ions released might play essential roles. We discuss further the possible molecular mechanisms underlying in the ion-induced angiogenic events.

show abstract

“…Figure b,c presents a cross-sectional view of 3D aligned scaffolds with 146 μm thickness, demonstrating the high porosity and excellent alignment of nanofibers. As the nanofiber porosity greatly affects the cell seeding into the inner space of 3D scaffolds, the ES time effect was investigated to control the nanofiber scaffold porosity . Three types of single-layer nanofiber scaffolds were produced (Figure d–f).…”

Section: Results and Discussionmentioning

confidence: 99%

3D Aligned Nanofiber Scaffold Fabrication with Trench-Guided Electrospinning for Cardiac Tissue Engineering

Qiu,

Wu,

Xue

et al. 2024

Langmuir

View full text Add to dashboard Cite

Constructing three-dimensional (3D) aligned nanofiber scaffolds is significant for the development of cardiac tissue engineering, which is promising in the field of drug discovery and disease mechanism study. However, the current nanofiber scaffold preparation strategy, which mainly includes manual assembly and hybrid 3D printing, faces the challenge of integrated fabrication of morphology-controllable nanofibers due to its cross-scale structural feature. In this research, a trench-guided electrospinning (ES) strategy was proposed to directly fabricate 3D aligned nanofiber scaffolds with alternative ES and a direct ink writing (DIW) process. The electric field effect of DIW poly(dimethylsiloxane) (PDMS) side walls on guiding whipping ES nanofibers was investigated to construct trench design rules. It was found that the width/height ratio of trenches greatly affected the nanofiber alignment, and the trench width/height ratio of 1.5 provided the nanofiber alignment degree over 60%. As a proof of principle, 3D nanofiber scaffolds with controllable porosity (60−80%) and alignment (30−60%) were fabricated. The effect of the scaffolds was verified by culturing human-induced pluripotent stem cellderived cardiomyocytes (hiPSC-CMs), which resulted in the uniform 3D distribution of aligned hiPSC-CMs with ∼1000 μm thickness. Therefore, this printing strategy shows great potential for the efficient engineered tissue construction.

show abstract

Thickness-controllable electrospun fibers promote tubular structure formation by endothelial progenitor cells

Cited by 11 publications

References 34 publications

Scaffolds and cells for tissue regeneration: different scaffold pore sizes—different cell effects

Scaffolds and cells for tissue regeneration: different scaffold pore sizes—different cell effects

A mini review focused on the proangiogenic role of silicate ions released from silicon-containing biomaterials

3D Aligned Nanofiber Scaffold Fabrication with Trench-Guided Electrospinning for Cardiac Tissue Engineering

Contact Info

Product

Resources

About